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However, responses to replication stress evoked by tight DNA\u2013protein complexes have not been fully elucidated. Here, we used bacterial LacI protein binding to lacO arrays to make site-specific replication fork barriers on the human chromosome. These barriers induced the accumulation of single-stranded DNA (ssDNA) and various DDR proteins at the lacO site. SLX4\u2013XPF functioned as an upstream factor for the accumulation of DDR proteins, and consequently, ATR and FANCD2 were interdependently recruited. Moreover, LacI binding in S phase caused underreplication and abnormal mitotic segregation of the lacO arrays. Finally, we show that the SLX4\u2013ATR axis represses the anaphase abnormality induced by LacI binding. Our results outline a long-term process by which human cells manage nucleoprotein obstacles ahead of the replication fork to prevent chromosomal instability.<\/jats:p>","DOI":"10.1083\/jcb.202003148","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T16:36:55Z","timestamp":1608568615000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":17,"title":["SLX4\u2013XPF mediates DNA damage responses to replication stress induced by DNA\u2013protein interactions"],"prefix":"10.1083","volume":"220","author":[{"given":"Riko","family":"Ishimoto","sequence":"first","affiliation":[{"name":"Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yota","family":"Tsuzuki","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoki","family":"Matsumura","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiichiro","family":"Kurashige","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8360-0102","authenticated-orcid":true,"given":"Kouki","family":"Enokitani","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koki","family":"Narimatsu","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsunori","family":"Higa","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nozomi","family":"Sugimoto","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0181-9857","authenticated-orcid":true,"given":"Kazumasa","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6617-2452","authenticated-orcid":true,"given":"Masatoshi","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2020,12,21]]},"reference":[{"key":"2023072401461442100_bib1","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.dnarep.2018.08.017","article-title":"Preserving replication fork integrity and competence via the homologous recombination pathway","volume":"71","author":"Ait Saada","year":"2018","journal-title":"DNA Repair (Amst.)."},{"key":"2023072401461442100_bib2","doi-asserted-by":"publisher","first-page":"35910","DOI":"10.1074\/jbc.M704645200","article-title":"Sequential and synergistic modification of human RPA stimulates chromosomal DNA repair","volume":"282","author":"Anantha","year":"2007","journal-title":"J. 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