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While molecular players modulating CME are well characterized, factors regulating ADBE and mechanisms coordinating CME and ADBE activations remain poorly understood. Here we report that Minibrain\/DYRK1A (Mnb), a kinase mutated in autism and up-regulated in Down\u2019s syndrome, plays a novel role in suppressing ADBE. We demonstrate that Mnb, together with calcineurin, delicately coordinates CME and ADBE by controlling the phosphoinositol phosphatase activity of synaptojanin (Synj) during varying synaptic demands. Functional domain analyses reveal that Synj\u2019s 5\u2032-phosphoinositol phosphatase activity suppresses ADBE, while SAC1 activity is required for efficient ADBE. Consequently, Parkinson\u2019s disease mutation in Synj\u2019s SAC1 domain impairs ADBE. These data identify Mnb and Synj as novel regulators of ADBE and further indicate that CME and ADBE are differentially governed by Synj\u2019s dual phosphatase domains.<\/jats:p>","DOI":"10.1083\/jcb.202011028","type":"journal-article","created":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T17:17:09Z","timestamp":1633108629000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":12,"title":["Minibrain kinase and calcineurin coordinate activity-dependent bulk endocytosis through synaptojanin"],"prefix":"10.1083","volume":"220","author":[{"given":"Yi-Jheng","family":"Peng","sequence":"first","affiliation":[{"name":"Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 1"},{"name":"Neuroscience Graduate Program, University of Southern California, Los Angeles, CA 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5817-8870","authenticated-orcid":true,"given":"Junhua","family":"Geng","sequence":"additional","affiliation":[{"name":"Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wu","sequence":"additional","affiliation":[{"name":"Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0826-5308","authenticated-orcid":true,"given":"Cristian","family":"Pinales","sequence":"additional","affiliation":[{"name":"Oak Crest Institute of Science, Monrovia, CA 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Langen","sequence":"additional","affiliation":[{"name":"Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen-Ching","family":"Chang","sequence":"additional","affiliation":[{"name":"Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4379-3878","authenticated-orcid":true,"given":"Christopher","family":"Buser","sequence":"additional","affiliation":[{"name":"Oak Crest Institute of Science, Monrovia, CA 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7619-8170","authenticated-orcid":true,"given":"Karen T.","family":"Chang","sequence":"additional","affiliation":[{"name":"Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 1"},{"name":"Neuroscience Graduate Program, University of Southern California, Los Angeles, CA 2"},{"name":"Department of Physiology & Neuroscience, Keck School of Medicine, University of Southern California, Los Angeles, CA 4"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2021,10,1]]},"reference":[{"key":"2023072801185332800_bib1","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1038\/nature20414","article-title":"Defects in trafficking bridge Parkinson\u2019s disease pathology and genetics","volume":"539","author":"Abeliovich","year":"2016","journal-title":"Nature."},{"key":"2023072801185332800_bib2","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/j.mcn.2008.10.005","article-title":"Enhancement of the endosomal endocytic pathway increases quantal size","volume":"40","author":"Akbergenova","year":"2009","journal-title":"Mol. 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