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Therefore, it is critical to understand macrophage infection, especially in tissue macrophages, which are widely infected in vivo, but poorly permissive to cell-free infection. Although cell-to-cell transmission of HIV-1 is a determinant mode of macrophage infection in vivo, how HIV-1 transfers toward macrophages remains elusive. Here, we demonstrate that fusion of infected CD4+ T lymphocytes with human macrophages leads to their efficient and productive infection. Importantly, several tissue macrophage populations undergo this heterotypic cell fusion, including synovial, placental, lung alveolar, and tonsil macrophages. We also find that this mode of infection is modulated by the macrophage polarization state. This fusion process engages a specific short-lived adhesion structure and is controlled by the CD81 tetraspanin, which activates RhoA\/ROCK-dependent actomyosin contractility in macrophages. Our study provides important insights into the mechanisms underlying infection of tissue-resident macrophages, and establishment of persistent cellular reservoirs in patients.<\/jats:p>","DOI":"10.1083\/jcb.202205103","type":"journal-article","created":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T02:21:25Z","timestamp":1675390885000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":37,"title":["Productive HIV-1 infection of tissue macrophages by fusion with infected CD4+ T cells"],"prefix":"10.1083","volume":"222","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7009-2786","authenticated-orcid":true,"given":"R\u00e9mi","family":"Mascarau","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/016zvc994","id-type":"ROR","asserted-by":"publisher"}],"name":"Institut de Pharmacologie et Biologie Structurale (IPBS), Universit\u00e9 de Toulouse, Centre National de la Recherche Scientifique, Universit\u00e9 Toulouse III - 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