{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:22:10Z","timestamp":1791339730517,"version":"4.3.1"},"reference-count":81,"publisher":"Rockefeller University Press","issue":"6","license":[{"start":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T00:00:00Z","timestamp":1680825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.rupress.org\/terms\/"},{"start":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T00:00:00Z","timestamp":1696550400000},"content-version":"vor","delay-in-days":182,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007914","name":"University of Texas Southwestern Medical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007914","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["I-2075-20210327"],"award-info":[{"award-number":["I-2075-20210327"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB1553334"],"award-info":[{"award-number":["MCB1553334"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention and Research Institute of Texas","doi-asserted-by":"publisher","award":["RP160157"],"award-info":[{"award-number":["RP160157"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI150715"],"award-info":[{"award-number":["AI150715"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,6,5]]},"abstract":"<jats:p>Accurate cellular replication balances the biogenesis and turnover of complex structures. In the apicomplexan parasite Toxoplasma gondii, daughter cells form within an intact mother cell, creating additional challenges to ensuring fidelity of division. The apical complex is critical to parasite infectivity and consists of apical secretory organelles and specialized cytoskeletal structures. We previously identified the kinase ERK7 as required for maturation of the apical complex in Toxoplasma. Here, we define the Toxoplasma ERK7 interactome, including a putative E3 ligase, CSAR1. Genetic disruption of CSAR1 fully suppresses loss of the apical complex upon ERK7 knockdown. Furthermore, we show that CSAR1 is normally responsible for turnover of maternal cytoskeleton during cytokinesis, and that its aberrant function is driven by mislocalization from the parasite residual body to the apical complex. These data identify a protein homeostasis pathway critical for Toxoplasma replication and fitness and suggest an unappreciated role for the parasite residual body in compartmentalizing processes that threaten the fidelity of parasite development.<\/jats:p>","DOI":"10.1083\/jcb.202209098","type":"journal-article","created":{"date-parts":[[2023,3,18]],"date-time":"2023-03-18T02:21:04Z","timestamp":1679106064000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":21,"title":["Toxoplasma ERK7 protects the apical complex from premature degradation"],"prefix":"10.1083","volume":"222","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6868-2648","authenticated-orcid":false,"given":"William J.","family":"O\u2019Shaughnessy","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05byvp690","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Texas, Southwestern Medical Center 1 Department of Pharmacology, , Dallas, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6030-0019","authenticated-orcid":true,"given":"Xiaoyu","family":"Hu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05byvp690","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Texas, Southwestern Medical Center 1 Department of Pharmacology, , Dallas, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8574-2748","authenticated-orcid":true,"given":"Sarah Ana","family":"Henriquez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05byvp690","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Texas, Southwestern Medical Center 1 Department of Pharmacology, , Dallas, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9401-9594","authenticated-orcid":true,"given":"Michael L.","family":"Reese","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05byvp690","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Texas, Southwestern Medical Center 1 Department of Pharmacology, , Dallas, TX, USA"},{"id":[{"id":"https:\/\/ror.org\/05byvp690","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Texas, Southwestern Medical Center 2 Department of Biochemistry, , Dallas, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2023,4,7]]},"reference":[{"key":"2024040700011960600_bib1","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1038\/nrm4084","article-title":"Control of microtubule organization and dynamics: Two ends in the limelight","volume":"16","author":"Akhmanova","year":"2015","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2024040700011960600_bib2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006483","article-title":"Checkpoints of apicomplexan cell division identified in Toxoplasma gondii","volume":"13","author":"Alvarez","year":"2017","journal-title":"PLoS Pathog."},{"key":"2024040700011960600_bib3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/B978-0-12-394309-5.00001-8","article-title":"Cytoskeleton assembly in Toxoplasma gondii cell division","volume":"298","author":"Anderson-White","year":"2012","journal-title":"Int. Rev. Cell Mol. Biol."},{"key":"2024040700011960600_bib4","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1111\/j.1462-5822.2010.01514.x","article-title":"A family of intermediate filament-like proteins is sequentially assembled into the cytoskeleton of Toxoplasma gondii","volume":"13","author":"Anderson-White","year":"2011","journal-title":"Cell. Microbiol."},{"key":"2024040700011960600_bib5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.exppara.2018.11.004","article-title":"Development and fate of the residual body of Toxoplasma gondii","volume":"196","author":"Attias","year":"2019","journal-title":"Exp. Parasitol."},{"key":"2024040700011960600_bib6","doi-asserted-by":"publisher","first-page":"12164","DOI":"10.1073\/pnas.1921245117","article-title":"Ancient MAPK ERK7 is regulated by an unusual inhibitory scaffold required for Toxoplasma apical complex biogenesis","volume":"117","author":"Back","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024040700011960600_bib7","doi-asserted-by":"publisher","first-page":"028644-21","DOI":"10.1128\/mbio.02864-21","article-title":"Multivalent interactions drive the Toxoplasma AC9:AC10:ERK7 complex to concentrate ERK7 in the apical cap","volume":"0286421","author":"Back","year":"2022","journal-title":"MBio"},{"key":"2024040700011960600_bib8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007946","article-title":"Toxoplasma F-box protein 1 is required for daughter cell scaffold function during parasite replication","volume":"15","author":"Baptista","year":"2019","journal-title":"PLoS Pathog."},{"key":"2024040700011960600_bib80","first-page":"920","article-title":"Elimination of false positives that arise in using the two-hybrid system","volume":"14","author":"Bartel","year":"1993","journal-title":"Biotechniques"},{"key":"2024040700011960600_bib9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001094","article-title":"A novel family of Toxoplasma IMC proteins displays a hierarchical organization and functions in coordinating parasite division","volume":"6","author":"Beck","year":"2010","journal-title":"PLoS Pathog."},{"key":"2024040700011960600_bib10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012354","article-title":"Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii","volume":"5","author":"Behnke","year":"2010","journal-title":"PLoS One"},{"key":"2024040700011960600_bib11","doi-asserted-by":"publisher","first-page":"9288","DOI":"10.1523\/JNEUROSCI.1582-17.2017","article-title":"The atypical MAP kinase SWIP-13\/ERK8 regulates dopamine transporters through a rho-dependent mechanism","volume":"37","author":"Bermingham","year":"2017","journal-title":"J. Neurosci."},{"key":"2024040700011960600_bib12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001020","article-title":"Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid","volume":"19","author":"Bertiaux","year":"2021","journal-title":"PLoS Biol."},{"key":"2024040700011960600_bib13","doi-asserted-by":"publisher","first-page":"4614","DOI":"10.1242\/jcs.122952","article-title":"Phosphorylation of chemoattractant receptors regulates chemotaxis, actin reorganization and signal relay","volume":"126","author":"Brzostowski","year":"2013","journal-title":"J. Cell Sci."},{"key":"2024040700011960600_bib14","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1046\/j.1365-2958.1999.01174.x","article-title":"Mobilization of intracellular calcium stimulates microneme discharge in Toxoplasma gondii","volume":"31","author":"Carruthers","year":"1999","journal-title":"Mol. Microbiol."},{"key":"2024040700011960600_bib15","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12678","article-title":"Novel insights into the composition and function of the Toxoplasma IMC sutures","volume":"19","author":"Chen","year":"2017","journal-title":"Cell. Microbiol."},{"key":"2024040700011960600_bib16","doi-asserted-by":"publisher","first-page":"3344","DOI":"10.1242\/jcs.123364","article-title":"The Toxoplasma gondii centrosome is the platform for internal daughter budding as revealed by a Nek1 kinase mutant","volume":"126","author":"Chen","year":"2013","journal-title":"J. Cell Sci."},{"key":"2024040700011960600_bib17","doi-asserted-by":"publisher","first-page":"1160","DOI":"10.1091\/mbc.E18-10-0608","article-title":"TgCep250 is dynamically processed through the division cycle and is essential for structural integrity of the Toxoplasma centrosome","volume":"30","author":"Chen","year":"2019","journal-title":"Mol. Biol. Cell"},{"key":"2024040700011960600_bib18","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.01828","article-title":"ERK8 is a negative regulator of O-GalNAc glycosylation and cell migration","volume":"3","author":"Chia","year":"2014","journal-title":"Elife"},{"key":"2024040700011960600_bib19","doi-asserted-by":"publisher","first-page":"1724","DOI":"10.4161\/auto.21857","article-title":"MAPK15\/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins","volume":"8","author":"Colecchia","year":"2012","journal-title":"Autophagy"},{"key":"2024040700011960600_bib20","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1111\/j.1462-5822.2009.01304.x","article-title":"Induction and regulation of conoid extrusion in Toxoplasma gondii","volume":"11","author":"Del Carmen","year":"2009","journal-title":"Cell. Microbiol."},{"key":"2024040700011960600_bib21","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1146\/annurev.biochem.78.101807.093809","article-title":"RING domain E3 ubiquitin ligases","volume":"78","author":"Deshaies","year":"2009","journal-title":"Annu. Rev. Biochem."},{"key":"2024040700011960600_bib22","doi-asserted-by":"publisher","first-page":"e00409","DOI":"10.1128\/mSphere.00409-17","article-title":"Differential roles for inner membrane complex proteins across Toxoplasma gondii and Sarcocystis neurona development","volume":"2","author":"Dubey","year":"2017","journal-title":"MSphere"},{"key":"2024040700011960600_bib23","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1038\/340245a0","article-title":"A novel genetic system to detect protein-protein interactions","volume":"340","author":"Fields","year":"1989","journal-title":"Nature"},{"key":"2024040700011960600_bib24","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/s13630-016-0025-5","article-title":"Basal body structure and composition in the apicomplexans Toxoplasma and Plasmodium","volume":"5","author":"Francia","year":"2016","journal-title":"Cilia"},{"key":"2024040700011960600_bib25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001444","article-title":"Cell division in Apicomplexan parasites is organized by a homolog of the striated rootlet fiber of algal flagella","volume":"10","author":"Francia","year":"2012","journal-title":"PLoS Biol."},{"key":"2024040700011960600_bib26","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1038\/nrmicro3184","article-title":"Cell division in apicomplexan parasites","volume":"12","author":"Francia","year":"2014","journal-title":"Nat. Rev. Microbiol."},{"key":"2024040700011960600_bib79","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1038\/ng0797-277","article-title":"Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens","volume":"16","author":"Fromont-Racine","year":"1997","journal-title":"Nat. Genet"},{"key":"2024040700011960600_bib27","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.protis.2016.12.001","article-title":"Budding of the alveolate alga vitrella brassicaformis resembles sexual and asexual processes in apicomplexan parasites","volume":"168","author":"F\u00fcssy","year":"2017","journal-title":"Protist"},{"key":"2024040700011960600_bib28","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1111\/j.1550-7408.1962.tb02610.x","article-title":"Electron microscope studies of reproducing and interkinetic Toxoplasma","volume":"9","author":"Gavin","year":"1962","journal-title":"J. Protozool."},{"key":"2024040700011960600_bib29","doi-asserted-by":"publisher","first-page":"161","DOI":"10.2307\/3274692","article-title":"Reproduction of Toxoplasma gondii by internal budding","volume":"44","author":"Goldman","year":"1958","journal-title":"J. Parasitol."},{"key":"2024040700011960600_bib30","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.1093\/molbev\/msq321","article-title":"Ciliate pellicular proteome identifies novel protein families with characteristic repeat motifs that are common to alveolates","volume":"28","author":"Gould","year":"2011","journal-title":"Mol. Biol. Evol."},{"key":"2024040700011960600_bib31","doi-asserted-by":"publisher","first-page":"1219","DOI":"10.1093\/molbev\/msn070","article-title":"Alveolins, a new family of cortical proteins that define the protist infrakingdom Alveolata","volume":"25","author":"Gould","year":"2008","journal-title":"Mol. Biol. Evol."},{"key":"2024040700011960600_bib32","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005388","article-title":"The conoid associated motor MyoH is indispensable for Toxoplasma gondii entry and exit from host cells","volume":"12","author":"Graindorge","year":"2016","journal-title":"PLoS Pathog."},{"key":"2024040700011960600_bib33","doi-asserted-by":"publisher","first-page":"670049","DOI":"10.3389\/fcimb.2021.670049","article-title":"The modular circuitry of apicomplexan cell division plasticity","volume":"11","author":"Gubbels","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"2024040700011960600_bib34","doi-asserted-by":"publisher","first-page":"269","DOI":"10.3389\/fcimb.2020.00269","article-title":"Fussing about fission: Defining variety among mainstream and exotic apicomplexan cell division modes","volume":"10","author":"Gubbels","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"2024040700011960600_bib35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020013","article-title":"Cytoskeletal components of an invasion machine--the apical complex of Toxoplasma gondii","volume":"2","author":"Hu","year":"2006","journal-title":"PLoS Pathog."},{"key":"2024040700011960600_bib36","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1091\/mbc.01-06-0309","article-title":"Daughter cell assembly in the protozoan parasite Toxoplasma gondii","volume":"13","author":"Hu","year":"2002","journal-title":"Mol. Biol. Cell"},{"key":"2024040700011960600_bib37","doi-asserted-by":"publisher","first-page":"1039","DOI":"10.1083\/jcb.200112086","article-title":"A novel polymer of tubulin forms the conoid of Toxoplasma gondii","volume":"156","author":"Hu","year":"2002","journal-title":"J. Cell Biol."},{"key":"2024040700011960600_bib38","doi-asserted-by":"publisher","first-page":"5697","DOI":"10.1242\/jcs.01494","article-title":"Variability and heritability of cell division pathways in Toxoplasma gondii","volume":"117","author":"Hu","year":"2004","journal-title":"J. Cell Sci."},{"key":"2024040700011960600_bib39","doi-asserted-by":"publisher","first-page":"e02517","DOI":"10.1128\/mBio.02517-20","article-title":"Loss of the conserved alveolate kinase MAPK2 decouples Toxoplasma cell growth from cell division","volume":"11","author":"Hu","year":"2020","journal-title":"MBio"},{"key":"2024040700011960600_bib40","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.50598","article-title":"Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii","volume":"8","author":"Hunt","year":"2019","journal-title":"Elife"},{"key":"2024040700011960600_bib41","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1128\/EC.00358-08","article-title":"Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80","volume":"8","author":"Huynh","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"2024040700011960600_bib42","doi-asserted-by":"publisher","first-page":"1423","DOI":"10.1534\/genetics.117.300383","article-title":"Primary cilium formation and ciliary protein trafficking is regulated by the atypical MAP kinase MAPK15 in caenorhabditis elegans and human cells","volume":"207","author":"Kazatskaya","year":"2017","journal-title":"Genetics"},{"key":"2024040700011960600_bib43","doi-asserted-by":"publisher","first-page":"E2453","DOI":"10.1073\/pnas.1406459111","article-title":"Probing nuclear pore complex architecture with proximity-dependent biotinylation","volume":"111","author":"Kim","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024040700011960600_bib44","doi-asserted-by":"publisher","first-page":"1188","DOI":"10.1091\/mbc.E15-12-0844","article-title":"An improved smaller biotin ligase for BioID proximity labeling","volume":"27","author":"Kim","year":"2016","journal-title":"Mol. Biol. Cell"},{"key":"2024040700011960600_bib45","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001081","article-title":"Molecular characterization of the conoid complex in Toxoplasma reveals its conservation in all apicomplexans, including Plasmodium species","volume":"19","author":"Koreny","year":"2021","journal-title":"PLoS Biol."},{"key":"2024040700011960600_bib46","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1111\/tra.12673","article-title":"The roles of Centrin 2 and Dynein Light Chain 8a in apical secretory organelles discharge of Toxoplasma gondii","volume":"20","author":"Lentini","year":"2019","journal-title":"Traffic"},{"key":"2024040700011960600_bib47","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1128\/EC.00096-13","article-title":"A SAS-6-like protein suggests that the Toxoplasma conoid complex evolved from flagellar components","volume":"12","author":"de Leon","year":"2013","journal-title":"Eukaryot. Cell"},{"key":"2024040700011960600_bib48","doi-asserted-by":"publisher","first-page":"27809","DOI":"10.1038\/srep27809","article-title":"An evolutionarily conserved SSNA1\/DIP13 homologue is a component of both basal and apical complexes of Toxoplasma gondii","volume":"6","author":"L\u00e9v\u00eaque","year":"2016","journal-title":"Sci. Rep."},{"key":"2024040700011960600_bib49","doi-asserted-by":"publisher","first-page":"2236","DOI":"10.1038\/s41467-017-02341-2","article-title":"A conserved ankyrin repeat-containing protein regulates conoid stability, motility and cell invasion in Toxoplasma gondii","volume":"8","author":"Long","year":"2017","journal-title":"Nat. Commun."},{"key":"2024040700011960600_bib50","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.1002\/cm.21077","article-title":"Targeted proteomic dissection of Toxoplasma cytoskeleton sub-compartments using MORN1","volume":"69","author":"Lorestani","year":"2012","journal-title":"Cytoskeleton"},{"key":"2024040700011960600_bib51","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/s0166-6851(01)00289-4","article-title":"Characterization of the subpellicular network, a filamentous membrane skeletal component in the parasite Toxoplasma gondii","volume":"115","author":"Mann","year":"2001","journal-title":"Mol. Biochem. Parasitol."},{"key":"2024040700011960600_bib52","doi-asserted-by":"publisher","first-page":"6666","DOI":"10.1038\/ncomms7666","article-title":"ERK7 regulates ciliogenesis by phosphorylating the actin regulator CapZIP in cooperation with Dishevelled","volume":"6","author":"Miyatake","year":"2015","journal-title":"Nat. Commun."},{"key":"2024040700011960600_bib53","doi-asserted-by":"publisher","first-page":"1017","DOI":"10.1242\/jcs.115.5.1017","article-title":"Disruption of microtubules uncouples budding and nuclear division in Toxoplasma gondii","volume":"115","author":"Morrissette","year":"2002","journal-title":"J. Cell Sci."},{"key":"2024040700011960600_bib54","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1091\/mbc.E16-08-0587","article-title":"Loss of a doublecortin (DCX)-domain protein causes structural defects in a tubulin-based organelle of Toxoplasma gondii and impairs host-cell invasion","volume":"28","author":"Nagayasu","year":"2017","journal-title":"Mol. Biol. Cell"},{"key":"2024040700011960600_bib55","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1111\/j.1550-7408.1987.tb03162.x","article-title":"Cytoskeleton of Toxoplasma gondii","volume":"34","author":"Nichols","year":"1987","journal-title":"J. Protozool."},{"key":"2024040700011960600_bib56","doi-asserted-by":"publisher","first-page":"1559","DOI":"10.1242\/jcs.021089","article-title":"Organellar dynamics during the cell cycle of Toxoplasma gondii","volume":"121","author":"Nishi","year":"2008","journal-title":"J. Cell Sci."},{"key":"2024040700011960600_bib57","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1038\/nmeth.1401","article-title":"An auxin-based degron system for the rapid depletion of proteins in nonplant cells","volume":"6","author":"Nishimura","year":"2009","journal-title":"Nat. Methods"},{"key":"2024040700011960600_bib58","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084653","article-title":"The 3D structure of the apical complex and association with the flagellar apparatus revealed by serial TEM tomography in Psammosa pacifica, a distant relative of the Apicomplexa","volume":"9","author":"Okamoto","year":"2014","journal-title":"PLoS One"},{"key":"2024040700011960600_bib59","doi-asserted-by":"publisher","first-page":"881","DOI":"10.1091\/mbc.E19-11-0607","article-title":"Loss of a conserved MAPK causes catastrophic failure in assembly of a specialized cilium-like structure in Toxoplasma gondii","volume":"31","author":"O\u2019Shaughnessy","year":"2020","journal-title":"Mol. Biol. Cell"},{"key":"2024040700011960600_bib81","doi-asserted-by":"crossref","unstructured":"O\u2019Shaughnessy, W.J., X.Hu, S.A.Henriquez, and M.L.Reese. 2023. Toxoplasma ERK7 defends the apical complex from premature degradation.bioRxiv. doi:10.1101\/2021.12.09.471932 (Preprint posted February 6, 2023).","DOI":"10.1083\/jcb.202209098"},{"key":"2024040700011960600_bib60","doi-asserted-by":"publisher","first-page":"3320","DOI":"10.1242\/jcs.147736","article-title":"Dynamics of the Toxoplasma gondii inner membrane complex","volume":"127","author":"Ouologuem","year":"2014","journal-title":"J. Cell Sci."},{"key":"2024040700011960600_bib61","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1038\/nature00946","article-title":"Golgi biogenesis in Toxoplasma gondii","volume":"418","author":"Pelletier","year":"2002","journal-title":"Nature"},{"key":"2024040700011960600_bib62","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.24119","article-title":"Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation","volume":"6","author":"Periz","year":"2017","journal-title":"Elife"},{"key":"2024040700011960600_bib63","doi-asserted-by":"publisher","first-page":"1415","DOI":"10.1038\/ncomms2449","article-title":"Spatial association with PTEX complexes defines regions for effector export into Plasmodium falciparum-infected erythrocytes","volume":"4","author":"Riglar","year":"2013","journal-title":"Nat. Commun."},{"key":"2024040700011960600_bib64","doi-asserted-by":"publisher","first-page":"23.1","DOI":"10.1002\/0471140864.ps1923s74","article-title":"BioID: A screen for protein-protein interactions","volume":"74","author":"Roux","year":"2013","journal-title":"Curr. Protoc. Protein Sci."},{"key":"2024040700011960600_bib65","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1083\/jcb.201112098","article-title":"A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells","volume":"196","author":"Roux","year":"2012","journal-title":"J. Cell Biol."},{"key":"2024040700011960600_bib66","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.38805","article-title":"Ancestral reconstruction reveals mechanisms of ERK regulatory evolution","volume":"8","author":"Sang","year":"2019","journal-title":"Elife"},{"key":"2024040700011960600_bib67","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"2024040700011960600_bib68","doi-asserted-by":"publisher","first-page":"1241","DOI":"10.1242\/jcs.113.7.1241","article-title":"Microtubules, but not actin filaments, drive daughter cell budding and cell division in Toxoplasma gondii","volume":"113","author":"Shaw","year":"2000","journal-title":"J. Cell Sci."},{"key":"2024040700011960600_bib69","doi-asserted-by":"publisher","first-page":"868","DOI":"10.1038\/s41564-022-01104-0","article-title":"Screening the Toxoplasma kinome with high-throughput tagging identifies a regulator of invasion and egress","volume":"7","author":"Smith","year":"2022","journal-title":"Nat. Microbiol."},{"key":"2024040700011960600_bib70","doi-asserted-by":"publisher","first-page":"1423","DOI":"10.1083\/jcb.151.7.1423","article-title":"The plastid of Toxoplasma gondii is divided by association with the centrosomes","volume":"151","author":"Striepen","year":"2000","journal-title":"J. Cell Biol."},{"key":"2024040700011960600_bib71","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1002093","article-title":"A novel bipartite centrosome coordinates the apicomplexan cell cycle","volume":"13","author":"Suvorova","year":"2015","journal-title":"PLoS Biol."},{"key":"2024040700011960600_bib72","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.56635","article-title":"Essential function of the alveolin network in the subpellicular microtubules and conoid assembly in Toxoplasma gondii","volume":"9","author":"Tosetti","year":"2020","journal-title":"Elife"},{"key":"2024040700011960600_bib73","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.42669","article-title":"Three F-actin assembly centers regulate organelle inheritance, cell-cell communication and motility in Toxoplasma gondii","volume":"8","author":"Tosetti","year":"2019","journal-title":"Elife"},{"key":"2024040700011960600_bib74","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1002\/cm.20469","article-title":"RNG1 is a late marker of the apical polar ring in Toxoplasma gondii","volume":"67","author":"Tran","year":"2010","journal-title":"Cytoskeleton"},{"key":"2024040700011960600_bib78","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1016\/s0076-6879(95)55036-4","article-title":"Ras-Raf interaction: two-hybrid analysis","volume":"255","author":"Vojtek","year":"1995","journal-title":"Methods Enzymol"},{"key":"2024040700011960600_bib75","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1128\/EC.00258-13","article-title":"Distinct roles of a mitogen-activated protein kinase in cytokinesis between different life cycle forms of Trypanosoma brucei","volume":"13","author":"Wei","year":"2014","journal-title":"Eukaryot. Cell"},{"key":"2024040700011960600_bib76","doi-asserted-by":"publisher","first-page":"4353","DOI":"10.1128\/IAI.70.8.4353-4361.2002","article-title":"Clostridium septicum alpha-toxin is active against the parasitic protozoan Toxoplasma gondii and targets members of the SAG family of glycosylphosphatidylinositol-anchored surface proteins","volume":"70","author":"Wichroski","year":"2002","journal-title":"Infect. Immun."},{"key":"2024040700011960600_bib77","doi-asserted-by":"publisher","first-page":"3684","DOI":"10.1038\/emboj.2011.253","article-title":"ERK7 is a negative regulator of protein secretion in response to amino-acid starvation by modulating Sec16 membrane association","volume":"30","author":"Zacharogianni","year":"2011","journal-title":"EMBO J."}],"container-title":["Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/doi\/10.1083\/jcb.202209098\/1450561\/jcb_202209098.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/doi\/10.1083\/jcb.202209098\/1450561\/jcb_202209098.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,6]],"date-time":"2024-04-06T20:01:35Z","timestamp":1712433695000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/222\/6\/e202209098\/214008\/Toxoplasma-ERK7-protects-the-apical-complex-from"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,7]]},"references-count":81,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6,5]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.202209098","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,7]]},"article-number":"e202209098"}}