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We developed a dynamic live-imaging platform with analytical capabilities to track subcellular structures throughout the parasite\u2019s 48-h intraerythrocytic life cycle. Our analysis revealed four distinct morphological stages in apicoplast development that correlate with nuclear replication. We identified a critical \u201cCrown\u201d morphology stage required for nucleus\u2013apicoplast attachment, where the apicoplast stretches across multiple nuclei, in close association with centriolar plaques. We measured DNA ploidy and replication dynamics of the nuclear and apicoplast genomes. Inhibition of nuclear DNA replication blocked apicoplast biogenesis at early stages, demonstrating dependence on S-phase initiation. Conversely, inhibiting apicoplast genome replication minimally affected organelle development but disrupted the Crown stage, preventing proper organelle segregation into daughter cells. 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