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While astrocytes exhibit distinct Ca2+ signaling patterns, decoding their physiological roles remains challenging because conventional approaches generate nonphysiological, spatially imprecise Ca2+ surges that obscure endogenous signals. Here, we developed a tunable light-based tool to modulate astrocytic Ca2+ activity, mimicking localized spikes and global waves elicited by graded glutamate through endogenous mGluR-Gq-IP3R signaling. IP3R triple knockout abolished light-evoked Ca2+ elevations, indicating elevated Ca2+ requires ER-IP3Rs. Using this modulation platform, we found that local Ca2+ spikes enhance organelle entry into astrocytic processes, whereas global Ca2+ waves arrest organelle movement. FKBP-FRB motor recruitment assays showed that global Ca2+ elevations acutely suppress motor-driven transport when cargo adaptors are bypassed. Combining our method with astrocyte process outgrowth analysis showed that Ca2+-dependent distal organelle accumulation promotes process elongation. Together, this light-based strategy provides a versatile platform for eliciting endogenous-like astrocytic Ca2+ patterns and reveals how distinct Ca2+ patterns differentially control organelle dynamics and astrocytic structural remodeling.<\/jats:p>","DOI":"10.1083\/jcb.202506032","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T06:21:21Z","timestamp":1777530081000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Light-based modulation of astrocytic calcium for regulation of organelle dynamics and morphogenesis"],"prefix":"10.1083","volume":"225","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5649-6044","authenticated-orcid":true,"given":"Lan","family":"Yang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04jth1r26","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Cellular Biology and Signaling, Zhejiang University-University of Edinburgh Institute, Zhejiang University, Haining, China 1"},{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"the Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University 2 Department of Neurology, , Hangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2176-681X","authenticated-orcid":true,"given":"Mikael","family":"Bj\u00f6rklund","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01nrxwf90","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Edinburgh Medical School, Biomedical Sciences, College of Medicine & Veterinary Medicine, University of Edinburgh 3 , Edinburgh,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6853-6563","authenticated-orcid":true,"given":"Cong","family":"Yi","sequence":"additional","affiliation":[{"name":"Zhejiang University School of Medicine 5 Department of Biochemistry and Department of Hepatobiliary and Pancreatic Surgery of the First Affiliated Hospital, , Hangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0061-8041","authenticated-orcid":true,"given":"Shue","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/004eeze55","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Reproductive Health Diseases Research and Translation of Ministry of Education, International Center for Aging and Cancer, Hainan Medical University 4 , Haikou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8977-9486","authenticated-orcid":true,"given":"Zhi","family":"Hong","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04jth1r26","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Cellular Biology and Signaling, Zhejiang University-University of Edinburgh Institute, Zhejiang University, Haining, China 1"},{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"the Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University 2 Department of Neurology, , Hangzhou,","place":["China"]},{"id":[{"id":"https:\/\/ror.org\/01nrxwf90","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Edinburgh Medical School, Biomedical Sciences, College of Medicine & Veterinary Medicine, University of Edinburgh 3 , Edinburgh,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"key":"2026051910362667400_bib1","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1038\/nrn1824","article-title":"Astrocyte-endothelial interactions at the blood-brain barrier","volume":"7","author":"Abbott","year":"2006","journal-title":"Nat. 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