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Golgiphagy, the selective autophagic degradation of the Golgi, has emerged as a key quality control pathway, but its physiological regulation in vivo remains largely unknown. Here, we generated two reporter mouse lines for constitutive or Cre-dependent visualization of Golgi architecture and Golgiphagy, enabling quantitative single-cell analysis across tissues. These models revealed striking cell type\u2013 and tissue-specific heterogeneity in basal Golgiphagy, including higher activity in renal proximal than distal tubules and in cerebellar than cortical neurons. Starvation and lipopolysaccharide-induced inflammation also promoted Golgi remodeling and Golgiphagy in multiple organs, particularly the liver, spleen, and kidney. Together, these reporter mice provide a powerful genetic toolkit for studying Golgi dynamics in vivo and establish that Golgiphagy is a spatially heterogeneous and stress-responsive process under physiological conditions.<\/jats:p>","DOI":"10.1083\/jcb.202508151","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T06:21:09Z","timestamp":1786083669000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Visualizing the Golgi apparatus and Golgiphagy in vivo"],"prefix":"10.1083","volume":"225","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4027-2547","authenticated-orcid":true,"given":"Pengwei","family":"Zhao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Metabolism Research, The Fourth Affiliated Hospital of Zhejiang University School of Medicine and International School of Medicine, 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