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In contrast, the well-studied yeast exocyst relies on multiple Rab GTPases to regulate function, but yeast do not encode Ral. Using Caenorhabditis elegans, we demonstrate that endogenous RAL-1 directly engages the exocyst through conserved binding sites in its subunits. Loss of RAL-1 disrupts dendritic arborization of PVD sensory neurons, impairs vesicle trafficking, and causes broad developmental defects, acting both cell autonomously in neurons and non-autonomously through supporting epithelial cells. Structure-guided genome editing of RAL-1\u2013exocyst interfaces produced synthetic phenotypes, underscoring the physiological importance of these contacts. Taken together, our findings establish RAL-1 as a bona fide regulator of the metazoan exocyst in vivo and suggest that Ral\u2013exocyst interactions operate in parallel with other secretory pathways. More broadly, this work positions C. elegans as a powerful system to dissect Ral\u2013exocyst mechanisms across molecular, cellular, and developmental scales.<\/jats:p>","DOI":"10.1083\/jcb.202508181","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T06:21:14Z","timestamp":1787293274000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["The Ral small GTPase is an essential regulator of exocyst complex function in secretion"],"prefix":"10.1083","volume":"225","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5535-1091","authenticated-orcid":true,"given":"You","family":"Wu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Vashisht College of Medicine, Texas A&M Health Science Center, Texas A&M University 1 , Houston, TX,","place":["USA"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University 2 , Houston, TX,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0344-7161","authenticated-orcid":true,"given":"David J.","family":"Reiner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Vashisht College of Medicine, Texas A&M Health Science Center, Texas A&M University 1 , Houston, TX,","place":["USA"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University 2 , Houston, TX,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"2026100609183537100_bib1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.65169","article-title":"A polarity pathway for exocyst-dependent intracellular tube extension","volume":"10","author":"Abrams","year":"2021","journal-title":"Elife"},{"key":"2026100609183537100_bib2","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1242\/dev.060939","article-title":"C. elegans bicd-1, homolog of the Drosophila dynein accessory factor Bicaudal D, regulates the branching of PVD sensory neuron dendrites","volume":"138","author":"Aguirre-Chen","year":"2011","journal-title":"Development"},{"key":"2026100609183537100_bib3","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/bs.ircmb.2020.10.005","article-title":"The RAL signaling network: Cancer and beyond","volume":"361","author":"Apken","year":"2021","journal-title":"Int. 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