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A key component of the AJC is a circumferential ring of F-actin, but how actomyosin contractility drives AJC structure and epithelial barrier function is incompletely understood. Here, we show that a central mechanosensitive component of zAJ, \u03b1-catenin (\u03b1-cat), undergoes force-dependent phosphorylation in an unstructured linker region. This modification in turn primes the \u03b1-cat middle region for effector binding. We credential afadin, a TJ\/AJ scaffold protein, as a mechanochemical binding partner of \u03b1-cat, identifying residues in \u03b1-cat required for binding. \u03b1-Cat phosphorylation and afadin binding support their co-enrichment at zAJ and barrier function. A mouse model that prevents \u03b1-cat phosphorylation is particularly detrimental to postnatal brain development, leading to hydrocephalus with junctional features similar to an afadin-binding mutant form of \u03b1-cat. These data support a model where \u03b1-cat integrates mechanical and chemical signals to progressively promote zAJ enrichment, effector recruitment, and barrier function.<\/jats:p>","DOI":"10.1083\/jcb.202508188","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T14:48:04Z","timestamp":1792507684000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["\u03b1-Catenin phosphorylation is tension-sensitive and supports epithelial barrier functions via afadin"],"prefix":"10.1083","volume":"225","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7830-4659","authenticated-orcid":true,"given":"Jeanne M.","family":"Quinn","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04fzwnh64","id-type":"ROR","asserted-by":"publisher"}],"name":"Northwestern University Feinberg School of Medicine 1 Department of Pulmonary Medicine, , Chicago, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0822-6668","authenticated-orcid":true,"given":"Phuong M.","family":"Le","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04fzwnh64","id-type":"ROR","asserted-by":"publisher"}],"name":"Northwestern University Feinberg School of Medicine 1 Department of Pulmonary Medicine, , Chicago, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3909-8861","authenticated-orcid":true,"given":"Anthea","family":"Weng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04fzwnh64","id-type":"ROR","asserted-by":"publisher"}],"name":"Northwestern University Feinberg School of Medicine 1 Department of Pulmonary Medicine, , Chicago, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0856-2670","authenticated-orcid":true,"given":"Annette S.","family":"Flozak","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04fzwnh64","id-type":"ROR","asserted-by":"publisher"}],"name":"Northwestern University Feinberg School of Medicine 1 Department of Pulmonary Medicine, , Chicago, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9778-9177","authenticated-orcid":true,"given":"S. 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