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However, the mechanisms underlying its formation remain elusive. In Drosophila, Myosin1D (Myo1D) and Myosin1C (Myo1C), respectively, dictate right-handed or left-handed chirality of the cell shape and body. Here, we report that Myo1D and Myo1C, respectively, directed clockwise and counterclockwise circumferential F-actin flows in Drosophila macrophages collaborating with Myosin2 (Myo2). Furthermore, Myo1C induced a random F-actin flow in an in vitro motility assay, whereas Myo1D triggered the self-organization of the F-actin ring rotating clockwise in the same conditions, implying that Myo1D assembles F-actin into a circular arrangement with barbed-end-to-pointed-end polarity aligned in a specific direction. We propose a model in which Myo1D induces the formation of F-actin structures aligned in such a polarized fashion within macrophages, and Myo2 rotates them clockwise. This model provides a molecular basis for the formation of cell and organ chirality.<\/jats:p>","DOI":"10.1083\/jcb.202509125","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T06:21:04Z","timestamp":1782973264000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Myosin1D directs circumferential F-actin flow to define cell chirality"],"prefix":"10.1083","volume":"225","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5078-0961","authenticated-orcid":true,"given":"Asuka","family":"Yamaguchi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}],"name":"Graduate School of Science, The University of Osaka 1 Department of Biological Sciences, , Toyonaka,","place":["Japan"]},{"id":[{"id":"https:\/\/ror.org\/02kpeqv85","id-type":"ROR","asserted-by":"publisher"}],"name":"Graduate School of Biostudies, Kyoto 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