{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:17:44Z","timestamp":1788445064432,"version":"build-2803163510"},"reference-count":68,"publisher":"Rockefeller University Press","issue":"10","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/rupress.org\/pages\/terms102024"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science\/MEXT KAKENHI","doi-asserted-by":"publisher","award":["JP18F18093"],"award-info":[{"award-number":["JP18F18093"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science\/MEXT KAKENHI","doi-asserted-by":"publisher","award":["JP20K22790"],"award-info":[{"award-number":["JP20K22790"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science\/MEXT KAKENHI","doi-asserted-by":"publisher","award":["JP23K14505"],"award-info":[{"award-number":["JP23K14505"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science\/MEXT KAKENHI","doi-asserted-by":"publisher","award":["JP26K18568"],"award-info":[{"award-number":["JP26K18568"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science\/MEXT KAKENHI","doi-asserted-by":"publisher","award":["JP18K06923"],"award-info":[{"award-number":["JP18K06923"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science\/MEXT KAKENHI","doi-asserted-by":"publisher","award":["JP21K06826"],"award-info":[{"award-number":["JP21K06826"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science\/MEXT KAKENHI","doi-asserted-by":"publisher","award":["JP25K11792"],"award-info":[{"award-number":["JP25K11792"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science\/MEXT KAKENHI","doi-asserted-by":"publisher","award":["JP18H02627"],"award-info":[{"award-number":["JP18H02627"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science\/MEXT KAKENHI","doi-asserted-by":"publisher","award":["JP19KK0199"],"award-info":[{"award-number":["JP19KK0199"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science\/MEXT KAKENHI","doi-asserted-by":"publisher","award":["JP21H04798"],"award-info":[{"award-number":["JP21H04798"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science\/MEXT KAKENHI","doi-asserted-by":"publisher","award":["JP24H00601"],"award-info":[{"award-number":["JP24H00601"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19gm1210006"],"award-info":[{"award-number":["JP19gm1210006"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP21wm0425008"],"award-info":[{"award-number":["JP21wm0425008"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP25gm1910006"],"award-info":[{"award-number":["JP25gm1910006"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003382","name":"Core Research for Evolutional Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003382","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012359","name":"Promotion and Mutual Aid Corporation for Private Schools of Japan","doi-asserted-by":"publisher","award":["S1411007"],"award-info":[{"award-number":["S1411007"]}],"id":[{"id":"10.13039\/501100012359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007807","name":"The Japan Science Society","doi-asserted-by":"publisher","award":["JSS-2020-134"],"award-info":[{"award-number":["JSS-2020-134"]}],"id":[{"id":"10.13039\/501100007807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,10,5]]},"abstract":"<jats:p>Various pathogenic microorganisms produce toxins that create pores in cell membranes, causing cell damage and disrupting the host epithelial barrier. Recently, we reported that mice lacking the G protein\u2013coupled receptor leukotriene B4 receptor 2 (BLT2), which is expressed in vascular endothelial and alveolar epithelial cells, are highly susceptible to pneumolysin (PLY), a pneumococci-generated toxin. Although we clarified the protective roles of BLT2 in vascular endothelial cells, those in alveolar epithelial cells have not been elucidated. Here, we report that lipid mediator 12-hydroxyheptadecatrienoic acid (12-HHT), which is produced by membrane-damaged epithelial cells, prevents cell death by promoting membrane repair through BLT2. BLT2 promoted the release of PLY-bound plasma membranes as extracellular vesicles in a sphingomyelinase-dependent manner. Additionally, BLT2 activated Rac1 and subsequent actin polymerization, leading to resistance to cell death. Furthermore, inhibition of 12-HHT production by aspirin and treatment with a BLT2 antagonist abolished the protective effect of BLT2. These findings provide a new therapeutic strategy for bacterial infection.<\/jats:p>","DOI":"10.1083\/jcb.202510004","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T06:21:03Z","timestamp":1785910863000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Lipid-mediated activation of BLT2 promotes membrane repair to prevent cell death"],"prefix":"10.1083","volume":"225","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2392-8578","authenticated-orcid":true,"given":"Yuan","family":"Chi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01692sz90","id-type":"ROR","asserted-by":"publisher"}],"name":"Juntendo University Graduate School of Medicine 1 Department of Biochemistry, , Tokyo,","place":["Japan"]},{"id":[{"id":"https:\/\/ror.org\/01692sz90","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Genomic and Regenerative Medicine, Juntendo University Graduate School of Medicine 2 , Tokyo,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1205-8261","authenticated-orcid":true,"given":"Kazuko","family":"Saeki","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01692sz90","id-type":"ROR","asserted-by":"publisher"}],"name":"Juntendo University Graduate School of Medicine 1 Department of Biochemistry, , Tokyo,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0425-342X","authenticated-orcid":true,"given":"Ken","family":"Yasukawa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01692sz90","id-type":"ROR","asserted-by":"publisher"}],"name":"Juntendo University Graduate School of Medicine 1 Department of Biochemistry, , Tokyo,","place":["Japan"]},{"name":"Sato Pharmaceutical Co., Ltd. 3 Drug Discovery Research Department, , Tokyo,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9434-4944","authenticated-orcid":true,"given":"Soichiro","family":"Kakuta","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01692sz90","id-type":"ROR","asserted-by":"publisher"}],"name":"Biomedical Research Core Facilities, Juntendo University Graduate School of Medicine 4 Laboratory of Morphology and Image Analysis, , Tokyo,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9584-2611","authenticated-orcid":true,"given":"Tomoaki","family":"Koga","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01692sz90","id-type":"ROR","asserted-by":"publisher"}],"name":"Juntendo University Graduate School of Medicine 1 Department of Biochemistry, , Tokyo,","place":["Japan"]},{"id":[{"id":"https:\/\/ror.org\/02cgss904","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Molecular Embryology and Genetics, Kumamoto University 5 Department of Medical Cell Biology, , Kumamoto,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5219-1553","authenticated-orcid":true,"given":"Takehiko","family":"Yokomizo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01692sz90","id-type":"ROR","asserted-by":"publisher"}],"name":"Juntendo University Graduate School of Medicine 1 Department of Biochemistry, , Tokyo,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"2026090309524966000_bib1","doi-asserted-by":"publisher","first-page":"2469","DOI":"10.1007\/s00018-012-0928-2","article-title":"Cytoskeleton responses in wound repair","volume":"69","author":"Abreu-Blanco","year":"2012","journal-title":"Cell. Mol. Life Sci."},{"key":"2026090309524966000_bib2","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1016\/j.bbamem.2014.11.023","article-title":"Aspirin inhibits formation of cholesterol rafts in fluid lipid membranes","volume":"1848","author":"Alsop","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"2026090309524966000_bib3","doi-asserted-by":"publisher","first-page":"R392","DOI":"10.1016\/j.cub.2017.12.034","article-title":"Plasma membrane repair","volume":"28","author":"Andrews","year":"2018","journal-title":"Curr. Biol."},{"key":"2026090309524966000_bib4","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1038\/nrm1155","article-title":"Calcium signalling: Dynamics, homeostasis and remodelling","volume":"4","author":"Berridge","year":"2003","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2026090309524966000_bib5","doi-asserted-by":"publisher","first-page":"1140","DOI":"10.3390\/toxins5061140","article-title":"Staphylococcus aureus \u03b1-toxin: Nearly a century of intrigue","volume":"5","author":"Berube","year":"2013","journal-title":"Toxins (Basel)"},{"key":"2026090309524966000_bib6","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1038\/emboj.2009.45","article-title":"Acid sphingomyelinase activity triggers microparticle release from glial cells","volume":"28","author":"Bianco","year":"2009","journal-title":"EMBO J."},{"key":"2026090309524966000_bib7","doi-asserted-by":"publisher","first-page":"2649","DOI":"10.1016\/j.bbamcr.2015.07.012","article-title":"Plasma membrane and cytoskeleton dynamics during single-cell wound healing","volume":"1853","author":"Boucher","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"2026090309524966000_bib8","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1038\/ncb1812","article-title":"MG53 nucleates assembly of cell membrane repair machinery","volume":"11","author":"Cai","year":"2009","journal-title":"Nat. Cell Biol."},{"key":"2026090309524966000_bib9","doi-asserted-by":"publisher","first-page":"1703244","DOI":"10.1080\/20013078.2019.1703244","article-title":"Inhibiting extracellular vesicles formation and release: A review of EV inhibitors","volume":"9","author":"Catalano","year":"2020","journal-title":"J. Extracell Vesicles"},{"key":"2026090309524966000_bib10","doi-asserted-by":"publisher","first-page":"1487","DOI":"10.1242\/jcs.139089","article-title":"Purinergic control of AMPK activation by ATP released through connexin 43 hemichannels - Pivotal roles in hemichannel-mediated cell injury","volume":"127","author":"Chi","year":"2014","journal-title":"J. Cell Sci."},{"key":"2026090309524966000_bib11","doi-asserted-by":"publisher","first-page":"5225","DOI":"10.1073\/pnas.97.10.5225","article-title":"Role of Rac in controlling the actin cytoskeleton and chemotaxis in motile cells","volume":"97","author":"Chung","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026090309524966000_bib12","doi-asserted-by":"publisher","first-page":"1205","DOI":"10.1152\/physrev.00037.2014","article-title":"Membrane repair: Mechanisms and pathophysiology","volume":"95","author":"Cooper","year":"2015","journal-title":"Physiol. Rev."},{"key":"2026090309524966000_bib13","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1038\/nrmicro.2015.3","article-title":"Pore-forming toxins: Ancient, but never really out of fashion","volume":"14","author":"Dal Peraro","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"2026090309524966000_bib14","doi-asserted-by":"publisher","first-page":"5756","DOI":"10.1073\/pnas.1722320115","article-title":"The glycolipid GM1 reshapes asymmetric biomembranes and giant vesicles by curvature generation","volume":"115","author":"Dasgupta","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026090309524966000_bib15","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/bs.ctm.2015.10.006","article-title":"Plasma membrane repair in health and disease","volume":"77","author":"Demonbreun","year":"2016","journal-title":"Curr. Top. Membr."},{"key":"2026090309524966000_bib16","doi-asserted-by":"publisher","first-page":"23036","DOI":"10.1074\/jbc.M211397200","article-title":"Membrane ruffling requires coordination between type Ialpha phosphatidylinositol phosphate kinase and Rac signaling","volume":"278","author":"Doughman","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2026090309524966000_bib17","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1007\/978-3-7091-1511-4_17","article-title":"Ceramide in plasma membrane repair","volume":"216","author":"Draeger","year":"2013","journal-title":"Handb. Exp. Pharmacol."},{"key":"2026090309524966000_bib18","doi-asserted-by":"publisher","first-page":"10886","DOI":"10.3390\/ijms221910886","article-title":"Actin cytoskeletal dynamics in single-cell wound repair","volume":"22","author":"Ebstrup","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"2026090309524966000_bib19","doi-asserted-by":"publisher","first-page":"234","DOI":"10.3390\/toxins10060234","article-title":"Membrane repair mechanisms against permeabilization by pore-forming toxins","volume":"10","author":"Etxaniz","year":"2018","journal-title":"Toxins (Basel)"},{"key":"2026090309524966000_bib20","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1007\/978-1-4419-6327-7_5","article-title":"Cholesterol-dependent cytolysins","volume":"677","author":"Gilbert","year":"2010","journal-title":"Adv. Exp. Med. Biol."},{"key":"2026090309524966000_bib21","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1007\/s00018-007-7434-y","article-title":"Bacterial pore-forming toxins: The (w)hole story?","volume":"65","author":"Gonzalez","year":"2008","journal-title":"Cell. Mol. Life Sci."},{"key":"2026090309524966000_bib22","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1007\/s00281-018-0701-1","article-title":"The first line of defence: Insights into mechanisms and relevance of phagocytosis in epithelial cells","volume":"40","author":"G\u00fcnther","year":"2018","journal-title":"Semin. Immunopathol."},{"key":"2026090309524966000_bib23","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1126\/science.279.5350.509","article-title":"Rho GTPases and the actin cytoskeleton","volume":"279","author":"Hall","year":"1998","journal-title":"Science"},{"key":"2026090309524966000_bib24","doi-asserted-by":"publisher","first-page":"3751","DOI":"10.1007\/s00018-018-2888-7","article-title":"Cellular mechanisms and signals that coordinate plasma membrane repair","volume":"75","author":"Horn","year":"2018","journal-title":"Cell. Mol. Life Sci."},{"key":"2026090309524966000_bib25","doi-asserted-by":"publisher","first-page":"1028","DOI":"10.1016\/j.bbamem.2011.07.036","article-title":"Membrane assembly of the cholesterol-dependent cytolysin pore complex","volume":"1818","author":"Hotze","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"2026090309524966000_bib26","doi-asserted-by":"publisher","first-page":"905","DOI":"10.1083\/jcb.200708010","article-title":"Repair of injured plasma membrane by rapid Ca2+-dependent endocytosis","volume":"180","author":"Idone","year":"2008","journal-title":"J. Cell Biol."},{"key":"2026090309524966000_bib27","doi-asserted-by":"publisher","first-page":"4678","DOI":"10.1096\/fj.10-165050","article-title":"Protective role of the leukotriene B4 receptor BLT2 in murine inflammatory colitis","volume":"24","author":"Iizuka","year":"2010","journal-title":"FASEB J."},{"key":"2026090309524966000_bib28","doi-asserted-by":"publisher","first-page":"24816","DOI":"10.1074\/jbc.M413257200","article-title":"Characterization of a mouse second leukotriene B4 receptor, mBLT2: BLT2-dependent ERK activation and cell migration of primary mouse keratinocytes","volume":"280","author":"Iizuka","year":"2005","journal-title":"J. Biol. Chem."},{"key":"2026090309524966000_bib29","doi-asserted-by":"publisher","first-page":"933","DOI":"10.1096\/fj.15-279653","article-title":"Leukotriene B4 receptor type 2 (BLT2) enhances skin barrier function by regulating tight junction proteins","volume":"30","author":"Ishii","year":"2016","journal-title":"FASEB J."},{"key":"2026090309524966000_bib30","first-page":"713","article-title":"12-HHT accelerates corneal wound healing through leukotriene B4 receptor 2 (BLT2)","volume":"56","author":"Iwamoto","year":"2015","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026090309524966000_bib31","doi-asserted-by":"publisher","first-page":"5085","DOI":"10.1523\/jneurosci.3560-12.2013","article-title":"Calpains, cleaved Mini-DysferlinC72, and L-Type channels underpin calcium-dependent muscle membrane repair","volume":"33","author":"Lek","year":"2013","journal-title":"J. Neurosci."},{"key":"2026090309524966000_bib32","doi-asserted-by":"publisher","first-page":"1215","DOI":"10.1172\/jci76693","article-title":"Annexin A1-containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair","volume":"125","author":"Leoni","year":"2015","journal-title":"J. Clin. Invest."},{"key":"2026090309524966000_bib33","doi-asserted-by":"publisher","first-page":"799","DOI":"10.1038\/nprot.2008.50","article-title":"Isolation and short-term culture of primary keratinocytes, hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for in vitro analysis and for grafting to immunodeficient mice","volume":"3","author":"Lichti","year":"2008","journal-title":"Nat. Protoc."},{"key":"2026090309524966000_bib34","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/j.immuni.2007.07.011","article-title":"Tumor suppressor CYLD regulates acute lung injury in lethal Streptococcus pneumoniae infections","volume":"27","author":"Lim","year":"2007","journal-title":"Immunity"},{"key":"2026090309524966000_bib35","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1084\/jem.20132063","article-title":"12-Hydroxyheptadecatrienoic acid promotes epidermal wound healing by accelerating keratinocyte migration via the BLT2 receptor","volume":"211","author":"Liu","year":"2014","journal-title":"J. Exp. Med."},{"key":"2026090309524966000_bib36","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1128\/MMBR.00052-12","article-title":"Role of pore-forming toxins in bacterial infectious diseases","volume":"77","author":"Los","year":"2013","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"2026090309524966000_bib37","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1038\/21658","article-title":"Characterization of the human cysteinyl leukotriene CysLT1 receptor","volume":"399","author":"Lynch","year":"1999","journal-title":"Nature"},{"key":"2026090309524966000_bib38","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1007\/978-1-4939-1752-5_8","article-title":"Cholesterol depletion using methyl-\u03b2-cyclodextrin","volume":"1232","author":"Mahammad","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"2026090309524966000_bib39","doi-asserted-by":"publisher","first-page":"35202","DOI":"10.1074\/jbc.M606406200","article-title":"Requirement for annexin A1 in plasma membrane repair","volume":"281","author":"McNeil","year":"2006","journal-title":"J. Biol. Chem."},{"key":"2026090309524966000_bib40","doi-asserted-by":"publisher","first-page":"13228","DOI":"10.1074\/jbc.m111.334524","article-title":"Characterization of EHop-016, novel small molecule inhibitor of Rac GTPase","volume":"287","author":"Montalvo-Ortiz","year":"2012","journal-title":"J. Biol. Chem."},{"key":"2026090309524966000_bib41","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1093\/oxfordjournals.jbchem.a134941","article-title":"Interaction of digitonin and its analogs with membrane cholesterol","volume":"96","author":"Nishikawa","year":"1984","journal-title":"J. Biochem."},{"key":"2026090309524966000_bib42","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/0378-1119(91)90434-d","article-title":"Efficient selection for high-expression transfectants with a novel eukaryotic vector","volume":"108","author":"Niwa","year":"1991","journal-title":"Gene"},{"key":"2026090309524966000_bib43","doi-asserted-by":"publisher","first-page":"1235","DOI":"10.1083\/jcb.144.6.1235","article-title":"Rho GTPases control polarity, protrusion, and adhesion during cell movement","volume":"144","author":"Nobes","year":"1999","journal-title":"J. Cell Biol."},{"key":"2026090309524966000_bib44","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1016\/j.bbrc.2018.06.026","article-title":"Profiling of bioactive lipids in different dendritic cell subsets using an improved multiplex quantitative LC-MS\/MS method","volume":"504","author":"Ohba","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026090309524966000_bib45","doi-asserted-by":"publisher","first-page":"41113","DOI":"10.1074\/jbc.m110.175497","article-title":"Leukotriene B4 augments and restores Fc gammaRs-dependent phagocytosis in macrophages","volume":"285","author":"Okamoto","year":"2010","journal-title":"J. Biol. Chem."},{"key":"2026090309524966000_bib46","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1084\/jem.20072329","article-title":"12(S)-Hydroxyheptadeca-5Z, 8E, 10E-trienoic acid is a natural ligand for leukotriene B4 receptor 2","volume":"205","author":"Okuno","year":"2008","journal-title":"J. Exp. Med."},{"key":"2026090309524966000_bib47","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1042\/BST20170435","article-title":"ESCRTs in membrane sealing","volume":"46","author":"Radulovic","year":"2018","journal-title":"Biochem. Soc. Trans."},{"key":"2026090309524966000_bib48","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abl6367","article-title":"Membrane repair triggered by cholesterol-dependent cytolysins is activated by mixed lineage kinases and MEK","volume":"8","author":"Ray","year":"2022","journal-title":"Sci. Adv."},{"key":"2026090309524966000_bib49","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/s0092-8674(01)00421-4","article-title":"Plasma membrane repair is mediated by Ca(2+)-regulated exocytosis of lysosomes","volume":"106","author":"Reddy","year":"2001","journal-title":"Cell"},{"key":"2026090309524966000_bib50","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1038\/cdd.2017.11","article-title":"Intrinsic repair protects cells from pore-forming toxins by microvesicle shedding","volume":"24","author":"Romero","year":"2017","journal-title":"Cell Death Differ."},{"key":"2026090309524966000_bib51","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.smim.2017.07.010","article-title":"Identification, signaling, and functions of LTB4 receptors","volume":"33","author":"Saeki","year":"2017","journal-title":"Semin. Immunol."},{"key":"2026090309524966000_bib52","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/j.ab.2012.03.014","article-title":"A high-affinity monoclonal antibody against the FLAG tag useful for G-protein-coupled receptor study","volume":"425","author":"Sasaki","year":"2012","journal-title":"Anal. Biochem."},{"key":"2026090309524966000_bib53","doi-asserted-by":"publisher","first-page":"34560","DOI":"10.1038\/srep34560","article-title":"Leukotriene B4 receptor type 2 protects against pneumolysin-dependent acute lung injury","volume":"6","author":"Shigematsu","year":"2016","journal-title":"Sci. Rep."},{"key":"2026090309524966000_bib54","doi-asserted-by":"publisher","first-page":"1859","DOI":"10.1126\/science.274.5294.1859","article-title":"Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore","volume":"274","author":"Song","year":"1996","journal-title":"Science"},{"key":"2026090309524966000_bib55","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1126\/science.7904084","article-title":"Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release","volume":"263","author":"Steinhardt","year":"1994","journal-title":"Science"},{"key":"2026090309524966000_bib56","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1038\/s43587-024-00575-6","article-title":"Plasma membrane damage limits replicative lifespan in yeast and induces premature senescence in human fibroblasts","volume":"4","author":"Suda","year":"2024","journal-title":"Nat. Aging"},{"key":"2026090309524966000_bib57","doi-asserted-by":"publisher","first-page":"20146","DOI":"10.3390\/molecules201119682","article-title":"Membrane disintegration caused by the Steroid Saponin Digitonin is related to the presence of cholesterol","volume":"20","author":"Sudji","year":"2015","journal-title":"Molecules"},{"key":"2026090309524966000_bib58","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1083\/jcb.201003053","article-title":"Exocytosis of acid sphingomyelinase by wounded cells promotes endocytosis and plasma membrane repair","volume":"189","author":"Tam","year":"2010","journal-title":"J. Cell Biol."},{"key":"2026090309524966000_bib59","doi-asserted-by":"publisher","first-page":"6199","DOI":"10.1128\/IAI.73.10.6199-6209.2005","article-title":"Cholesterol-dependent cytolysins, a family of versatile pore-forming toxins","volume":"73","author":"Tweten","year":"2005","journal-title":"Infect Immun"},{"key":"2026090309524966000_bib60","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1099\/mic.0.001154","article-title":"Bacterial pore-forming toxins","volume":"168","author":"Ulhuq","year":"2022","journal-title":"Microbiology (Reading)"},{"key":"2026090309524966000_bib61","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.23644","article-title":"CryoEM structures of membrane pore and prepore complex reveal cytolytic mechanism of pneumolysin","volume":"6","author":"van Pee","year":"2017","journal-title":"Elife"},{"key":"2026090309524966000_bib62","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.1194\/jlr.R083915","article-title":"Role of sphingolipids in the biogenesis and biological activity of extracellular vesicles","volume":"59","author":"Verderio","year":"2018","journal-title":"J. Lipid Res."},{"key":"2026090309524966000_bib63","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1093\/jb\/mvac105","article-title":"Identification and characterization of bioactive metabolites of 12-hydroxyheptadecatrienoic acid, a ligand for leukotriene B4 receptor 2","volume":"173","author":"Yasukawa","year":"2023","journal-title":"J. Biochem."},{"key":"2026090309524966000_bib64","doi-asserted-by":"publisher","first-page":"620","DOI":"10.1038\/42506","article-title":"A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis","volume":"387","author":"Yokomizo","year":"1997","journal-title":"Nature"},{"key":"2026090309524966000_bib65","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1084\/jem.192.3.421","article-title":"A second leukotriene B(4) receptor, BLT2. A new therapeutic target in inflammation and immunological disorders","volume":"192","author":"Yokomizo","year":"2000","journal-title":"J. Exp. Med."},{"key":"2026090309524966000_bib66","doi-asserted-by":"publisher","first-page":"2691","DOI":"10.1172\/JCI97946","article-title":"Leukotriene receptors as potential therapeutic targets","volume":"128","author":"Yokomizo","year":"2018","journal-title":"J. Clin Invest."},{"key":"2026090309524966000_bib67","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1111\/imr.13196","article-title":"The leukotriene B4 receptors BLT1 and BLT2 as potential therapeutic targets","volume":"317","author":"Yokomizo","year":"2023","journal-title":"Immunol. Rev."},{"key":"2026090309524966000_bib68","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020106922","article-title":"Sealing holes in cellular membranes","volume":"40","author":"Zhen","year":"2021","journal-title":"EMBO J."}],"container-title":["Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/doi\/10.1083\/jcb.202510004\/2040548\/jcb_202510004.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/doi\/10.1083\/jcb.202510004\/2040548\/jcb_202510004.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T13:53:08Z","timestamp":1788443588000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/225\/10\/e202510004\/282996\/Lipid-mediated-activation-of-BLT2-promotes"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,3]]},"references-count":68,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,5]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.202510004","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,3]]},"article-number":"e202510004"}}