{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T02:33:33Z","timestamp":1787711613823,"version":"build-2784847793"},"reference-count":45,"publisher":"Rockefeller University Press","issue":"9","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Government of Ireland Postgraduate Scholarship","award":["GOIPG\/2023\/3987"],"award-info":[{"award-number":["GOIPG\/2023\/3987"]}]},{"name":"Research Ireland Frontiers for the Future Award","award":["20\/FFP-A\/8519"],"award-info":[{"award-number":["20\/FFP-A\/8519"]}]},{"DOI":"10.13039\/100018998","name":"Irish Research eLibrary","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018998","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,9,7]]},"abstract":"<jats:p>The centromere is the chromosomal site of kinetochore assembly, defined by the histone H3 variant CENP-A. In each cell cycle, the assembly and maintenance of CENP-A is functionally critical for chromosome segregation. In Drosophila male meiosis, CID (fly CENP-A) is assembled in two phases: prophase of meiosis I and postmeiosis II. Here, we investigate the dynamics of the assembly components CAL1 and CENP-C in prophase I and determine the requirements for the second assembly phase. In early prophase I, CENP-C functions with CAL1 to maintain the centromere. In late prophase I, CAL1 is undetectable at centromeres and CENP-C is not critical for centromere maintenance. Instead, CENP-C is crucial for meiotic kinetochore recruitment and function. This CENP-C pool also functions in CID assembly postmeiosis II, which is independent of CAL1. In addition to different functional pools of CENP-C, distinct pools of the CID protein persist in the male germline, and the synthesis of each pool is uncoupled from its cell cycle deposition timing.<\/jats:p>","DOI":"10.1083\/jcb.202512053","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T06:21:17Z","timestamp":1781158877000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":1,"title":["Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis"],"prefix":"10.1083","volume":"225","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9134-003X","authenticated-orcid":true,"given":"Rachel S.","family":"Keegan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03bea9k73","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Chromosome Biology, University of Galway 1 , Galway,","place":["Ireland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7308-8846","authenticated-orcid":true,"given":"Dina","family":"Malkeyeva","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03bea9k73","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Chromosome Biology, University of Galway 1 , Galway,","place":["Ireland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7895-4950","authenticated-orcid":true,"given":"Meg B.","family":"Weever","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03bea9k73","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Chromosome Biology, University of Galway 1 , Galway,","place":["Ireland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6885-0421","authenticated-orcid":true,"given":"Elaine M.","family":"Dunleavy","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03bea9k73","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Chromosome Biology, University of Galway 1 , Galway,","place":["Ireland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"2026063009105327700_bib1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008997","article-title":"How noncrossover homologs are conjoined and segregated in Drosophila male meiosis I: Stable but reversible homolog linkers require a novel separase target protein","volume":"16","author":"Adams","year":"2020","journal-title":"PLoS Genet."},{"key":"2026063009105327700_bib2","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1038\/NRG2466","article-title":"Epigenetic regulation of centromeric chromatin: Old dogs, new tricks?","volume":"9","author":"Allshire","year":"2008","journal-title":"Nat. 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