{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:52:16Z","timestamp":1791208336582,"version":"4.1.0"},"reference-count":67,"publisher":"Rockefeller University Press","issue":"11","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/rupress.org\/pages\/terms102024"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM136291"],"award-info":[{"award-number":["R35GM136291"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32GM144636-01"],"award-info":[{"award-number":["T32GM144636-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Holden Comprehensive Cancer Center","award":["P30CA086862"],"award-info":[{"award-number":["P30CA086862"]}],"id":[{"id":"https:\/\/ror.org\/01jhe7086","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["25PRE1365873"],"award-info":[{"award-number":["25PRE1365873"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,11,2]]},"abstract":"<jats:p>All cells experience mechanical forces that shape their behavior and organization. These forces are sensed at cell\u2013cell adhesions and transduced through the actin cytoskeleton, triggering cytoskeletal reinforcement to withstand mechanical load. Such remodeling is energy-intensive and supported by increased glycolysis, yet the mechanisms linking mechanical stress to metabolic activation remain incomplete. Here, we identify phosphofructokinase-1 (PFK1) as a bridge coupling mechanotransduction and metabolism. Shear stress activates PFK1 and promotes its association with F-actin, enhancing reinforcement, glucose uptake, and intracellular ATP generation. Among the three human PFK1 isoforms, only the muscle isoform (PFKM) is essential for this response. The PFKM C-terminal tail is required for complete actin engagement and mechanosensitive activation: deletion abolishes this function, while grafting the tail onto other isoforms restores it. These findings establish PFKM as a mechanosensitive metabolic enzyme that links cytoskeletal mechanics to energy production, providing a framework for how mechanical and metabolic dysfunctions intersect in disease.<\/jats:p>","DOI":"10.1083\/jcb.202512133","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T06:21:15Z","timestamp":1787120475000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Distinct mechanical regulation of PFK1 isoforms during cytoskeletal remodeling"],"prefix":"10.1083","volume":"225","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0777-8336","authenticated-orcid":true,"given":"Logan W.","family":"Dawson","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Iowa Roy J. and Lucille A. Carver College of Medicine 1 Department of Biochemistry, , Iowa City, IA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9606-5815","authenticated-orcid":true,"given":"Nicholas M.","family":"Cronin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Iowa Roy J. and Lucille A. Carver College of Medicine 1 Department of Biochemistry, , Iowa City, IA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1575-3586","authenticated-orcid":true,"given":"Corentin C.G.","family":"Charles","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Iowa Roy J. and Lucille A. Carver College of Medicine 1 Department of Biochemistry, , Iowa City, IA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4750-5554","authenticated-orcid":true,"given":"Mallory R.","family":"Tollefson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Iowa Roy J. and Lucille A. Carver College of Medicine 1 Department of Biochemistry, , Iowa City, IA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7467-4719","authenticated-orcid":true,"given":"Megan C.","family":"Latham","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Iowa Roy J. and Lucille A. Carver College of Medicine 1 Department of Biochemistry, , Iowa City, IA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0906-1371","authenticated-orcid":true,"given":"Kris A.","family":"DeMali","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Iowa Roy J. and Lucille A. Carver College of Medicine 1 Department of Biochemistry, , Iowa City, IA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"key":"2026100508580960900_bib1","doi-asserted-by":"publisher","first-page":"3301","DOI":"10.1242\/jcs.107.12.3301","article-title":"ATP depletion: A novel method to study junctional properties in epithelial tissues. I. Rearrangement of the actin cytoskeleton","volume":"107","author":"Bacallao","year":"1994","journal-title":"J. Cell Sci."},{"key":"2026100508580960900_bib2","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1089\/wound.2016.0709","article-title":"Mechanical forces in cutaneous wound healing: Emerging therapies to minimize scar formation","volume":"7","author":"Barnes","year":"2018","journal-title":"Adv. Wound Care (New Rochelle)"},{"key":"2026100508580960900_bib3","doi-asserted-by":"publisher","first-page":"724","DOI":"10.1038\/ncb3537","article-title":"Linking E-cadherin mechanotransduction to cell metabolism through force-mediated activation of AMPK","volume":"19","author":"Bays","year":"2017","journal-title":"Nat. Cell Biol."},{"key":"2026100508580960900_bib4","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1083\/jcb.201309092","article-title":"Vinculin phosphorylation differentially regulates mechanotransduction at cell-cell and cell-matrix adhesions","volume":"205","author":"Bays","year":"2014","journal-title":"J. Cell Biol."},{"key":"2026100508580960900_bib5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1523\/jneurosci.23-01-00002.2003","article-title":"Actin-ATP hydrolysis is a major energy drain for neurons","volume":"23","author":"Bernstein","year":"2003","journal-title":"J. Neurosci."},{"key":"2026100508580960900_bib6","doi-asserted-by":"publisher","first-page":"16","DOI":"10.3390\/cancers16010016","article-title":"Hitting the sweet spot: How glucose metabolism is orchestrated in space and time by Phosphofructokinase-1","volume":"16","author":"Campos","year":"2023","journal-title":"Cancers (Basel)"},{"key":"2026100508580960900_bib7","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1042\/BCJ20253024","article-title":"The more we learn, the more diverse it gets: Structures, functions and evolution in the phosphofructokinase superfamily","volume":"482","author":"Compton","year":"2025","journal-title":"Biochem. J."},{"key":"2026100508580960900_bib8","doi-asserted-by":"publisher","DOI":"10.1101\/2024.05.09.593171","article-title":"Mechanical activation of VE-cadherin stimulates AMPK to increase endothelial cell metabolism and vasodilation","author":"Cronin","year":"2024","journal-title":"bioRxiv"},{"key":"2026100508580960900_bib9","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1111\/j.1432-1033.1986.tb09631.x","article-title":"Nucleotide exchange between cytosolic ATP and F-actin-bound ADP may be a major energy-utilizing process in unstimulated platelets","volume":"156","author":"Daniel","year":"1986","journal-title":"Eur. J. Biochem."},{"key":"2026100508580960900_bib10","doi-asserted-by":"publisher","first-page":"102219","DOI":"10.1016\/j.ceb.2023.102219","article-title":"Mechanotransduction: Forcing a change in metabolism","volume":"84","author":"Dawson","year":"2023","journal-title":"Curr. Opin. Cell Biol."},{"key":"2026100508580960900_bib11","doi-asserted-by":"publisher","first-page":"jcs248385","DOI":"10.1242\/jcs.248385","article-title":"Fueling the cytoskeleton - Links between cell metabolism and actin remodeling","volume":"134","author":"Dewane","year":"2021","journal-title":"J. Cell Sci."},{"key":"2026100508580960900_bib12","doi-asserted-by":"publisher","first-page":"G757","DOI":"10.1152\/ajpgi.2000.279.4.G757","article-title":"Rotavirus alters paracellular permeability and energy metabolism in Caco-2 cells","volume":"279","author":"Dickman","year":"2000","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"2026100508580960900_bib13","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1016\/j.tibs.2004.09.004","article-title":"Actin-binding proteins--a unifying hypothesis","volume":"29","author":"Dominguez","year":"2004","journal-title":"Trends Biochem. Sci."},{"key":"2026100508580960900_bib14","doi-asserted-by":"publisher","first-page":"jcs260985","DOI":"10.1242\/jcs.260985","article-title":"Response of cells and tissues to shear stress","volume":"136","author":"Espina","year":"2023","journal-title":"J. Cell Sci."},{"key":"2026100508580960900_bib15","doi-asserted-by":"publisher","first-page":"1377","DOI":"10.1007\/s11095-007-9527-6","article-title":"Myosin light chain kinase inhibition: Correction of increased intestinal epithelial permeability in vitro","volume":"25","author":"Feighery","year":"2008","journal-title":"Pharm. Res."},{"key":"2026100508580960900_bib16","doi-asserted-by":"publisher","first-page":"4425","DOI":"10.1042\/BCJ20200656","article-title":"Biochemical and transcript level differences between the three human phosphofructokinases show optimisation of each isoform for specific metabolic niches","volume":"477","author":"Fernandes","year":"2020","journal-title":"Biochem. J."},{"key":"2026100508580960900_bib17","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.yjmcc.2021.08.013","article-title":"In vivo deep network tracing reveals phosphofructokinase-mediated coordination of biosynthetic pathway activity in the myocardium","volume":"162","author":"Fulghum","year":"2022","journal-title":"J. Mol. Cell Cardiol."},{"key":"2026100508580960900_bib18","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1016\/s1096-7192(03)00037-4","article-title":"Epinephrine modulates cellular distribution of muscle phosphofructokinase","volume":"78","author":"Gomes Alves","year":"2003","journal-title":"Mol. Genet. Metab."},{"key":"2026100508580960900_bib19","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1042\/bj2690685","article-title":"Control of glycolysis in cultured chick embryo hepatocytes. Fructose 2,6-bisphosphate content and phosphofructokinase-1 activity are stimulated by insulin and epidermal growth factor","volume":"269","author":"Hamer","year":"1990","journal-title":"Biochem. J."},{"key":"2026100508580960900_bib20","doi-asserted-by":"publisher","first-page":"6754","DOI":"10.1038\/s41467-022-34095-x","article-title":"Altered glycolysis triggers impaired mitochondrial metabolism and mTORC1 activation in diabetic \u03b2-cells","volume":"13","author":"Haythorne","year":"2022","journal-title":"Nat. Commun."},{"key":"2026100508580960900_bib21","doi-asserted-by":"publisher","first-page":"55613","DOI":"10.3791\/55613","article-title":"Measuring protein binding to F-actin by Co-sedimentation","volume":"18","author":"Heier","year":"2017","journal-title":"J. Vis. Exp."},{"key":"2026100508580960900_bib22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000658","article-title":"A unique role for nonmuscle myosin heavy chain IIA in regulation of epithelial apical junctions","volume":"2","author":"Ivanov","year":"2007","journal-title":"PLoS One"},{"key":"2026100508580960900_bib23","doi-asserted-by":"publisher","first-page":"512","DOI":"10.2353\/ajpath.2010.100168","article-title":"Cytoskeletal regulation of epithelial barrier function during inflammation","volume":"177","author":"Ivanov","year":"2010","journal-title":"Am. J. Pathol."},{"key":"2026100508580960900_bib24","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1038\/nrm2597","article-title":"Mechanotransduction gone awry","volume":"10","author":"Jaalouk","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2026100508580960900_bib25","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2016.81","article-title":"Regulation and function of AMPK in physiology and diseases","volume":"48","author":"Jeon","year":"2016","journal-title":"Exp. Mol. Med."},{"key":"2026100508580960900_bib26","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"2026100508580960900_bib27","doi-asserted-by":"publisher","first-page":"106090","DOI":"10.1016\/j.isci.2023.106090","article-title":"Actin crosslinking by \u03b1-actinin averts viscous dissipation of myosin force transmission in stress fibers","volume":"26","author":"Katsuta","year":"2023","journal-title":"iScience"},{"key":"2026100508580960900_bib28","doi-asserted-by":"publisher","first-page":"2743","DOI":"10.1083\/jcb.201811127","article-title":"Cross-linker-mediated regulation of actin network organization controls tissue morphogenesis","volume":"218","author":"Krueger","year":"2019","journal-title":"J. Cell Biol."},{"key":"2026100508580960900_bib29","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1111\/j.1432-1033.1972.tb01785.x","article-title":"The self-association of rabbit-muscle phosphofructokinase","volume":"26","author":"Leonard","year":"1972","journal-title":"Eur. J. Biochem."},{"key":"2026100508580960900_bib30","doi-asserted-by":"publisher","first-page":"18890","DOI":"10.1074\/jbc.M115.647941","article-title":"Structural determinants of the mechanical stability of \u03b1-Catenin","volume":"290","author":"Li","year":"2015","journal-title":"J. Biol. Chem."},{"key":"2026100508580960900_bib31","doi-asserted-by":"publisher","first-page":"6736","DOI":"10.1038\/s41467-025-62111-3","article-title":"PFKM phosphorylates histone H3 and promotes mitotic progression by sensing the levels of citrate","volume":"16","author":"Lin","year":"2025","journal-title":"Nat. Commun."},{"key":"2026100508580960900_bib32","doi-asserted-by":"publisher","first-page":"282","DOI":"10.20892\/j.issn.2095-3941.2019.0437","article-title":"Role of the mechanical microenvironment in cancer development and progression","volume":"17","author":"Liu","year":"2020","journal-title":"Cancer Biol. Med."},{"key":"2026100508580960900_bib33","doi-asserted-by":"publisher","first-page":"7323","DOI":"10.1038\/s41467-024-51808-6","article-title":"Structural basis for allosteric regulation of human phosphofructokinase-1","volume":"15","author":"Lynch","year":"2024","journal-title":"Nat. Commun."},{"key":"2026100508580960900_bib34","doi-asserted-by":"publisher","first-page":"G875","DOI":"10.1152\/ajpgi.2000.279.5.g875","article-title":"Cytochalasin B modulation of Caco-2 tight junction barrier: Role of myosin light chain kinase","volume":"279","author":"Ma","year":"2000","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"2026100508580960900_bib35","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1002\/1097-0029(20001015)51:2&lt;156::AID-JEMT7&gt;3.0.CO;2-J","article-title":"Mechanism of extracellular calcium regulation of intestinal epithelial tight junction permeability: Role of cytoskeletal involvement","volume":"51","author":"Ma","year":"2000","journal-title":"Microsc. Res. Tech."},{"key":"2026100508580960900_bib36","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.ymeth.2015.09.025","article-title":"Using magnets and magnetic beads to dissect signaling pathways activated by mechanical tension applied to cells","volume":"94","author":"Marjoram","year":"2016","journal-title":"Methods"},{"key":"2026100508580960900_bib37","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1016\/s0960-9822(00)00742-9","article-title":"Phosphorylation and activation of heart PFK-2 by AMPK has a role in the stimulation of glycolysis during ischaemia","volume":"10","author":"Marsin","year":"2000","journal-title":"Curr. Biol."},{"key":"2026100508580960900_bib38","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202011162","article-title":"Synaptopodin stress fiber and contractomere at the epithelial junction","volume":"221","author":"Morris","year":"2022","journal-title":"J. Cell Biol."},{"key":"2026100508580960900_bib39","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1007\/s10911-009-9151-7","article-title":"Classic studies of mammary development and milk secretion: 1945 - 1980","volume":"14","author":"Neville","year":"2009","journal-title":"J. Mammary Gland Biol. Neoplasia"},{"key":"2026100508580960900_bib40","doi-asserted-by":"publisher","first-page":"5171","DOI":"10.3390\/ijms20205171","article-title":"AMPK activation promotes tight junction assembly in intestinal epithelial Caco-2 cells","volume":"20","author":"Olivier","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"2026100508580960900_bib41","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1038\/s41586-020-1998-1","article-title":"Mechanical regulation of glycolysis via cytoskeleton architecture","volume":"578","author":"Park","year":"2020","journal-title":"Nature"},{"key":"2026100508580960900_bib42","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1161\/circresaha.108.175331","article-title":"Mechanical control of tissue morphogenesis","volume":"103","author":"Patwari","year":"2008","journal-title":"Circ. Res."},{"key":"2026100508580960900_bib43","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.devcel.2018.10.021","article-title":"Mechanical force-driven adherens junction remodeling and epithelial dynamics","volume":"47","author":"Pinheiro","year":"2018","journal-title":"Dev. Cell"},{"key":"2026100508580960900_bib44","doi-asserted-by":"publisher","first-page":"a018226","DOI":"10.1101\/cshperspect.a018226","article-title":"Actin and actin-binding proteins","volume":"8","author":"Pollard","year":"2016","journal-title":"Cold Spring Harbor Perspect. Biol."},{"key":"2026100508580960900_bib45","doi-asserted-by":"publisher","first-page":"3437","DOI":"10.1021\/bi00386a028","article-title":"Binding of phosphofructokinase to filamentous actin","volume":"26","author":"Roberts","year":"1987","journal-title":"Biochemistry"},{"key":"2026100508580960900_bib46","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1016\/0003-9861(89)90110-0","article-title":"Properties of the interaction between phosphofructokinase and actin","volume":"269","author":"Roberts","year":"1989","journal-title":"Arch. Biochem. Biophys."},{"key":"2026100508580960900_bib47","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1002\/cm.20547","article-title":"Regulation of epithelial permeability by the actin cytoskeleton","volume":"68","author":"Rodgers","year":"2011","journal-title":"Cytoskeleton (Hoboken)"},{"key":"2026100508580960900_bib48","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1038\/s41556-021-00677-y","article-title":"Ankyrin G organizes membrane components to promote coupling of cell mechanics and glucose uptake","volume":"23","author":"Salvi","year":"2021","journal-title":"Nat. Cell Biol."},{"key":"2026100508580960900_bib49","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.ceb.2018.05.004","article-title":"Mechanisms linking mechanotransduction and cell metabolism","volume":"54","author":"Salvi","year":"2018","journal-title":"Curr. Opin. Cell Biol."},{"key":"2026100508580960900_bib50","doi-asserted-by":"publisher","first-page":"738","DOI":"10.1111\/jcmm.13417","article-title":"Physical forces modulate cell differentiation and proliferation processes","volume":"22","author":"Schwartz","year":"2018","journal-title":"J. Cell Mol. Med."},{"key":"2026100508580960900_bib51","doi-asserted-by":"publisher","first-page":"1379","DOI":"10.1007\/s10439-011-0252-2","article-title":"Biomechanical forces shape the tumor microenvironment","volume":"39","author":"Shieh","year":"2011","journal-title":"Ann. Biomed. Eng."},{"key":"2026100508580960900_bib52","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1177\/2211068214561025","article-title":"TEER measurement techniques for in vitro barrier model systems","volume":"20","author":"Srinivasan","year":"2015","journal-title":"J. Lab Autom"},{"key":"2026100508580960900_bib53","doi-asserted-by":"publisher","DOI":"10.1126\/science.aal3321","article-title":"A subcellular map of the human proteome","volume":"356","author":"Thul","year":"2017","journal-title":"Science"},{"key":"2026100508580960900_bib54","first-page":"105","article-title":"Tarui disease and distal glycogenoses: Clinical and genetic update","volume":"26","author":"Toscano","year":"2007","journal-title":"Acta Myol."},{"key":"2026100508580960900_bib55","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.1038\/sj.emboj.7600605","article-title":"E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctions","volume":"24","author":"Tunggal","year":"2005","journal-title":"EMBO J."},{"key":"2026100508580960900_bib56","doi-asserted-by":"publisher","first-page":"1260419","DOI":"10.1126\/science.1260419","article-title":"Proteomics. Tissue-based map of the human proteome","volume":"347","author":"Uhl\u00e9n","year":"2015","journal-title":"Science"},{"key":"2026100508580960900_bib57","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/s0167-4889(98)00152-9","article-title":"Structural modules in actin-binding proteins: Towards a new classification","volume":"1448","author":"Van Troys","year":"1999","journal-title":"Biochim. Biophys. Acta"},{"key":"2026100508580960900_bib58","doi-asserted-by":"publisher","first-page":"393","DOI":"10.3389\/fphys.2013.00393","article-title":"First description of phosphofructokinase deficiency in Spain: Identification of a novel homozygous missense mutation in the PFKM gene","volume":"4","author":"Vives-Corrons","year":"2013","journal-title":"Front. Physiol."},{"key":"2026100508580960900_bib59","doi-asserted-by":"publisher","first-page":"2305","DOI":"10.1083\/jcb.201701084","article-title":"The glycolytic enzyme phosphofructokinase-1 assembles into filaments","volume":"216","author":"Webb","year":"2017","journal-title":"J. Cell Biol."},{"key":"2026100508580960900_bib60","doi-asserted-by":"publisher","first-page":"120494","DOI":"10.1016\/j.biomaterials.2020.120494","article-title":"Energy expenditure during cell spreading influences the cellular response to matrix stiffness","volume":"267","author":"Xie","year":"2021","journal-title":"Biomaterials"},{"key":"2026100508580960900_bib61","doi-asserted-by":"publisher","first-page":"H1477","DOI":"10.1152\/ajpheart.00204.2018","article-title":"Shear stress augments mitochondrial ATP generation that triggers ATP release and Ca2+ signaling in vascular endothelial cells","volume":"315","author":"Yamamoto","year":"2018","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"2026100508580960900_bib62","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1038\/s41421-024-00744-2","article-title":"TPM4 condensates glycolytic enzymes and facilitates actin reorganization under hyperosmotic stress","volume":"10","author":"Yang","year":"2024","journal-title":"Cell Discov."},{"key":"2026100508580960900_bib63","doi-asserted-by":"publisher","first-page":"873","DOI":"10.1186\/s12967-025-06897-2","article-title":"Phosphofructokinase-1 redefined: A metabolic hub orchestrating cancer hallmarks through multi-dimensional control networks","volume":"23","author":"Yuan","year":"2025","journal-title":"J. Transl. Med."},{"key":"2026100508580960900_bib64","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1002\/iub.58","article-title":"ATP and fructose-2,6-bisphosphate regulate skeletal muscle 6-phosphofructo-1-kinase by altering its quaternary structure","volume":"60","author":"Zancan","year":"2008","journal-title":"IUBMB Life"},{"key":"2026100508580960900_bib65","doi-asserted-by":"publisher","first-page":"4185","DOI":"10.1038\/s41467-019-12155-z","article-title":"Energetic costs regulated by cell mechanics and confinement are predictive of migration path during decision-making","volume":"10","author":"Zanotelli","year":"2019","journal-title":"Nat. Commun."},{"key":"2026100508580960900_bib66","doi-asserted-by":"publisher","first-page":"5563","DOI":"10.1038\/s41467-025-60596-6","article-title":"Self-organizing glycolytic waves tune cellular metabolic states and fuel cancer progression","volume":"16","author":"Zhan","year":"2025","journal-title":"Nat. Commun."},{"key":"2026100508580960900_bib67","doi-asserted-by":"publisher","first-page":"1801","DOI":"10.1242\/jcs.001586","article-title":"Force activates smooth muscle alpha-actin promoter activity through the rho signaling pathway","volume":"120","author":"Zhao","year":"2007","journal-title":"J. Cell Sci."}],"container-title":["Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/doi\/10.1083\/jcb.202512133\/2041405\/jcb_202512133.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/doi\/10.1083\/jcb.202512133\/2041405\/jcb_202512133.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:58:30Z","timestamp":1791205110000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/225\/11\/e202512133\/283045\/Distinct-mechanical-regulation-of-PFK1-isoforms"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,5]]},"references-count":67,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11,2]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.202512133","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,5]]},"article-number":"e202512133"}}