{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:08:30Z","timestamp":1760044110457},"reference-count":0,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1967,1,1]]},"abstract":"<jats:p>A self-contained mechanical system for circulating nutrient fluid through 12 tissue culture chambers is described in detail. This system utilizes nonperforated cellophane membranes in the chambers which separate the circulating nutrient from the tissue culture environments. The nutrient, therefore, is dialyzed through the cellophane of each chamber; some cell products are retained in the microenvironment between the closely apposed cellophane and cover slip, whereas the other cell products move from chamber to chamber in the circulating nutrient. The resultant environmental conditions directed by the circumfusion systems are highly favorable for maintaining the differentiation of chick embryo tissues over protracted periods; a number of micrographs are shown.<\/jats:p>","DOI":"10.1083\/jcb.32.1.89","type":"journal-article","created":{"date-parts":[[2004,5,14]],"date-time":"2004-05-14T22:01:30Z","timestamp":1084572090000},"page":"89-112","update-policy":"http:\/\/dx.doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":26,"title":["THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS"],"prefix":"10.1083","volume":"32","author":[{"given":"George G.","family":"Rose","sequence":"first","affiliation":[{"name":"From the Department of Biology, The University of Texas M. D. Anderson Hospital and Tumor Institute; the Department of Medicine (Periodontics), The University of Texas Dental Branch; and the Tissue Culture Laboratory, Hermann Hospital, Houston"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1967,1,1]]},"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/32\/1\/89\/1619425\/89.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/32\/1\/89\/1619425\/89.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T01:04:01Z","timestamp":1690160641000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/32\/1\/89\/16897\/THE-CIRCUMFUSION-SYSTEM-FOR-MULTIPURPOSE-CULTURE"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1967,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1967,1,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.32.1.89","relation":{},"ISSN":["1540-8140","0021-9525"],"issn-type":[{"value":"1540-8140","type":"electronic"},{"value":"0021-9525","type":"print"}],"subject":[],"published":{"date-parts":[[1967,1,1]]}}}