{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T07:40:49Z","timestamp":1698824449126},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1968,11,1]]},"abstract":"<jats:p>Tritiated thymidine routinely labels onion root cytoplasm during most of the cell cycle. One-third of this label could be cytochemically identified as DNA. The balance of the label was not RNA or a lipid, or attributable to labeled impurities in thymidine-3H. In electron microscope radioautographs one-third of the cytoplasmic silver grains was over organelles, presumably mitochondria and plastids. The other two-thirds of the silver grains in electron micrographs was distributed widely, 41% over ground cytoplasm and 10% over cell walls-cell membranes. Snake venom phosphodiesterase (SVDase) extracted a cytoplasmic fraction not degraded by DNase, and did not appear to extract nuclear DNA. The SVDase-extractable fraction may be DNA or a thymidine 5'-phosphoryl group in an ester linkage with another hydroxylic compound. The nature of the nonextractable fraction is considered. Possibilities discussed are: (1) technical problems such as the binding of an acid-labile nuclear DNA in the cytoplasm; (2) non-DNA, such as breakdown products, and thymine compounds other than DNA; (3) DNA, not extractable because of the nature of its binding to other compounds or because it is a \"core\" resistant to DNase. Until the chemical nature of this nonextractable fraction is known, cytoplasmic label following thymidine-3H treatment cannot necessarily be considered DNA, nor the assumption made that thymidine-3H exclusively labels DNA.<\/jats:p>","DOI":"10.1083\/jcb.39.2.264","type":"journal-article","created":{"date-parts":[[2004,5,14]],"date-time":"2004-05-14T22:01:30Z","timestamp":1084572090000},"page":"264-285","update-policy":"http:\/\/dx.doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":7,"title":["CYTOPLASMIC LABEL FOLLOWING TRITIATED THYMIDINE TREATMENT OF <i>ALLIUM CEPA<\/i> L. ROOTS"],"prefix":"10.1083","volume":"39","author":[{"given":"Catharine P.","family":"Fussell","sequence":"first","affiliation":[{"name":"From the Department of Botany, Columbia University, New York."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1968,11,1]]},"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/39\/2\/264\/1621088\/264.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/39\/2\/264\/1621088\/264.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T01:42:08Z","timestamp":1690162928000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/39\/2\/264\/1543\/CYTOPLASMIC-LABEL-FOLLOWING-TRITIATED-THYMIDINE"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,11,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1968,11,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.39.2.264","relation":{},"ISSN":["1540-8140","0021-9525"],"issn-type":[{"value":"1540-8140","type":"electronic"},{"value":"0021-9525","type":"print"}],"subject":[],"published":{"date-parts":[[1968,11,1]]}}}