{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T01:35:46Z","timestamp":1789954546792,"version":"4.0.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1974,2,1]]},"abstract":"<jats:p>The phorbol ester 12-0-tetradecanoyl-phorbol-13-acetate, a potent tumor-promoting agent, caused irreversible platelet aggregation when more than 0.02 \u00b5M was stirred with human citrated or heparinized platelet-rich plasma (PRP). With washed platelets, 1 nM was effective. The alcohol phorbol, which has little tumor-promoting activity, failed to cause platelet aggregation. With all but low concentrations of phorbol ester, aggregation was succeeded by a rapid phase. The latter was prevented or reduced by enzymes which destroy ADP and by aspirin, was associated with a change in platelet shape, and was presumably due to released ADP. At higher concentrations, only a rapid phase was seen, and these inhibitors were not effective. Low concentrations did not aggregate platelets in PRP containing sufficient EDTA or EGTA to chelate ionized calcium or in PRP from thrombasthenic patients; higher concentrations caused slight aggregation. Both the primary, non-ADP-dependent aggregation and the rapid ADP-dependent aggregation were markedly inhibited by substances which increase cyclic AMP, metabolic inhibitors, and the sulfhydryl inhibitor N-ethylmaleimide. Phorbol ester reduced platelet cyclic AMP only when it had been previously elevated by prostaglandin E1. 1 \u00b5M did not release \u00df-glucuronidase, lactic dehydrogenase, or inflammatory material from platelets in 4\u20135 min despite marked aggregation, but liberated all three in 30 min. The possibility is discussed that low phorbol ester concentrations cause primary aggregation by a direct action on platelet actomyosin.<\/jats:p>","DOI":"10.1083\/jcb.60.2.325","type":"journal-article","created":{"date-parts":[[2004,5,14]],"date-time":"2004-05-14T22:01:30Z","timestamp":1084572090000},"page":"325-336","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":80,"title":["THE TUMOR-PROMOTER PHORBOL ESTER (12-0-TETRADECANOYL-PHORBOL-13-ACETATE), A POTENT AGGREGATING AGENT FOR BLOOD PLATELETS"],"prefix":"10.1083","volume":"60","author":[{"given":"Marjorie B.","family":"Zucker","sequence":"first","affiliation":[{"name":"From the Departments of Pathology and Environmental Medicine, New York University School of Medicine, New York 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Troll","sequence":"additional","affiliation":[{"name":"From the Departments of Pathology and Environmental Medicine, New York University School of Medicine, New York 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sidney","family":"Belman","sequence":"additional","affiliation":[{"name":"From the Departments of Pathology and Environmental Medicine, New York University School of Medicine, New York 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1974,2,1]]},"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/60\/2\/325\/1628596\/325.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/60\/2\/325\/1628596\/325.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T04:27:05Z","timestamp":1690172825000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/60\/2\/325\/18295\/THE-TUMOR-PROMOTER-PHORBOL-ESTER-12-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1974,2,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.60.2.325","relation":{},"ISSN":["1540-8140","0021-9525"],"issn-type":[{"value":"1540-8140","type":"electronic"},{"value":"0021-9525","type":"print"}],"subject":[],"published":{"date-parts":[[1974,2,1]]}}}