{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T08:56:09Z","timestamp":1762160169547},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1975,2,1]]},"abstract":"<jats:p>Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin-antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.<\/jats:p>","DOI":"10.1083\/jcb.64.2.331","type":"journal-article","created":{"date-parts":[[2004,5,14]],"date-time":"2004-05-14T23:04:59Z","timestamp":1084575899000},"page":"331-339","update-policy":"http:\/\/dx.doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":101,"title":["Two improved methods for preparing ferritin-protein conjugates for electron microscopy."],"prefix":"10.1083","volume":"64","author":[{"given":"Y","family":"Kishida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B R","family":"Olsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R A","family":"Berg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D J","family":"Prockop","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1975,2,1]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/64\/2\/331\/1629826\/331.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/64\/2\/331\/1629826\/331.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T06:32:46Z","timestamp":1690180366000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/64\/2\/331\/18516\/Two-improved-methods-for-preparing-ferritin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1975,2,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.64.2.331","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,2,1]]}}}