{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:57:28Z","timestamp":1790866648013,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1981,2,1]]},"abstract":"<jats:p>The transcription of three specific genes has been examined in heat-shocked drosophila cells by hybridizing pulse-labeled nuclear RNA with cloned DNA sequences. Actin gene transcription is rapidly and profoundly suppressed upon heat shock but returns to near- normal levels after cells are placed back at their normal culture temperature (23 degrees C). Conversely, the transcription of genes coding from 70,000- and 26,000-dalton heat- shock proteins increases dramatically and with extraordinary rapidity (60 s) after heat shock. The temporal patterns of 70,000- and 26,000-dalton heat-shock gene transcription are nearly superimposable, indicating that, although they are closely linked cytologically, these genes are nevertheless tightly coregulated. The abundance of heat- shock gene transcripts reaches remarkable levels, e.g., 70,000-dalton heat-shock gene transcripts account for 2-3 percent of the nuclear RNA labeled during the first 30 min of heat shock. When heat-shocked cells are returned to 25 degrees C, the rates of transcription of the heat-shock genes fall back to the low levels characteristic of untreated cells. To confirm the low level of heat-shock gene transcription in normal cells, nuclear RNA was purified from unlabeled (and otherwise unhandled) 25 degrees C cells, end-labeled in vitro with (32)P, and hybridized to cloned heat-shock DNA sequences. These and other data establish that the genes for 70,000- and 26,000-dalton heat-shock proteins in culture drosophila cells are active at 25 degrees C, and that their rate of transcription is greatly accelerated upon heat shock rather than being activated from a true \"off\" state. The rapidity, magnitude, and reversibility of the shifts in actin and heat-shock gene transcription constitute compelling advantages for the use of cultured drosophila cells in studying the transcriptional regulation of eukaryotic genes, including one related to the cytoskeleton.<\/jats:p>","DOI":"10.1083\/jcb.88.2.323","type":"journal-article","created":{"date-parts":[[2004,5,14]],"date-time":"2004-05-14T19:04:59Z","timestamp":1084561499000},"page":"323-328","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":75,"title":["Regulated transcription of the genes for actin and heat-shock proteins in culture drosophila cells"],"prefix":"10.1083","volume":"88","author":[{"given":"RC","family":"Findly","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Pederson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1981,2,1]]},"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/88\/2\/323\/1637960\/323.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/88\/2\/323\/1637960\/323.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T05:38:03Z","timestamp":1690177083000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/88\/2\/323\/20940\/Regulated-transcription-of-the-genes-for-actin-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1981,2,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.88.2.323","relation":{},"ISSN":["1540-8140","0021-9525"],"issn-type":[{"value":"1540-8140","type":"electronic"},{"value":"0021-9525","type":"print"}],"subject":[],"published":{"date-parts":[[1981,2,1]]}}}