{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T18:58:18Z","timestamp":1785869898454,"version":"3.56.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,11,1]]},"abstract":"<jats:p>Programmed cell death (PCD) plays a key role in developmental biology and in maintenance of the steady state in continuously renewing tissues. Currently, its existence is inferred mainly from gel electrophoresis of a pooled DNA extract as PCD was shown to be associated with DNA fragmentation. Based on this observation, we describe here the development of a method for the in situ visualization of PCD at the single-cell level, while preserving tissue architecture. Conventional histological sections, pretreated with protease, were nick end labeled with biotinylated poly dU, introduced by terminal deoxy-transferase, and then stained using avidin-conjugated peroxidase. The reaction is specific, only nuclei located at positions where PCD is expected are stained. The initial screening includes: small and large intestine, epidermis, lymphoid tissues, ovary, and other organs. A detailed analysis revealed that the process is initiated at the nuclear periphery, it is relatively short (1-3 h from initiation to cell elimination) and that PCD appears in tissues in clusters. The extent of tissue-PCD revealed by this method is considerably greater than apoptosis detected by nuclear morphology, and thus opens the way for a variety of studies.<\/jats:p>","DOI":"10.1083\/jcb.119.3.493","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T00:22:22Z","timestamp":1084580542000},"page":"493-501","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":7176,"title":["Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation."],"prefix":"10.1083","volume":"119","author":[{"given":"Y","family":"Gavrieli","sequence":"first","affiliation":[{"name":"Department of Experimental Medicine, Hebrew University-Hadassah Medical School, Jerusalem, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Sherman","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, Hebrew University-Hadassah Medical School, Jerusalem, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S A","family":"Ben-Sasson","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, Hebrew University-Hadassah Medical School, Jerusalem, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1992,11,1]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/119\/3\/493\/1473332\/493.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/119\/3\/493\/1473332\/493.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T02:50:26Z","timestamp":1689994226000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/119\/3\/493\/14516\/Identification-of-programmed-cell-death-in-situ"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1992,11,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.119.3.493","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,11,1]]}}}