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Functional analysis using fluorescence resonance energy transfer revealed that hDcp1a and hDcp2 interact in vivo in these structures that were shown to differ from the previously described stress granules. Our data indicate that these new structures are dynamic, as they disappear when mRNA breakdown is abolished by treatment with inhibitors. Accumulation of poly(A)+ RNA in these structures, after RNAi-mediated inactivation of the Xrn1 exonuclease, demonstrates that they represent active mRNA decay sites. The occurrence of 5\u2032\u20133\u2032 mRNA decay in specific subcellular locations in human cells suggests that the cytoplasm of eukaryotic cells may be more organized than previously anticipated.<\/jats:p>","DOI":"10.1083\/jcb.200309008","type":"journal-article","created":{"date-parts":[[2004,4,6]],"date-time":"2004-04-06T00:54:11Z","timestamp":1081212851000},"page":"31-40","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":513,"title":["Cytoplasmic foci are sites of mRNA decay in human cells"],"prefix":"10.1083","volume":"165","author":[{"given":"Nicolas","family":"Cougot","sequence":"first","affiliation":[{"name":"E\u0301quipe labellise\u0301e La Ligue, Centre de Ge\u0301ne\u0301tique Mole\u0301culaire, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Babajko","sequence":"additional","affiliation":[{"name":"E\u0301quipe labellise\u0301e La Ligue, Centre de Ge\u0301ne\u0301tique Mole\u0301culaire, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Se\u0301raphin","sequence":"additional","affiliation":[{"name":"E\u0301quipe labellise\u0301e La Ligue, Centre de Ge\u0301ne\u0301tique Mole\u0301culaire, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2004,4,5]]},"reference":[{"key":"2023072905254271400_BIB1","first-page":"3444","volume":"55","year":"1995","journal-title":"Cancer Res."},{"key":"2023072905254271400_BIB2","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1046\/j.1365-2443.2003.00665.x","volume":"8","year":"2003","journal-title":"Genes Cells."},{"key":"2023072905254271400_BIB3","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1093\/nar\/28.3.809","volume":"28","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"2023072905254271400_BIB4","doi-asserted-by":"crossref","first-page":"2148","DOI":"10.1101\/gad.13.16.2148","volume":"13","year":"1999","journal-title":"Genes Dev."},{"key":"2023072905254271400_BIB5","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1083\/jcb.136.4.761","volume":"136","year":"1997","journal-title":"J. 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