{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:04:54Z","timestamp":1773288294565,"version":"3.50.1"},"reference-count":38,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,9,13]]},"abstract":"<jats:p>We investigated in different human cell types nuclear positioning and transcriptional regulation of the functionally unrelated genes GASZ, CFTR, and CORTBP2, mapping to adjacent loci on human chromosome 7q31. When inactive, GASZ, CFTR, and CORTBP2 preferentially associated with the nuclear periphery and with perinuclear heterochromatin, whereas in their actively transcribed states the gene loci preferentially associated with euchromatin in the nuclear interior. Adjacent genes associated simultaneously with these distinct chromatin fractions localizing at different nuclear regions, in accordance with their individual transcriptional regulation. Although the nuclear localization of CFTR changed after altering its transcription levels, the transcriptional status of CFTR was not changed by driving this gene into a different nuclear environment. This implied that the transcriptional activity affected the nuclear positioning, and not vice versa. Together, the results show that small chromosomal subregions can display highly flexible nuclear organizations that are regulated at the level of individual genes in a transcription-dependent manner.<\/jats:p>","DOI":"10.1083\/jcb.200404107","type":"journal-article","created":{"date-parts":[[2004,9,13]],"date-time":"2004-09-13T19:12:58Z","timestamp":1095102778000},"page":"815-825","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":229,"title":["Transcription-dependent spatial arrangements of CFTR and adjacent genes in human cell nuclei"],"prefix":"10.1083","volume":"166","author":[{"given":"Daniele","family":"Zink","sequence":"first","affiliation":[{"name":"1Ludwig Maximilians University Munich, Department of Biology II, 80336 Munich, Germany"}]},{"given":"Margarida D.","family":"Amaral","sequence":"additional","affiliation":[{"name":"2Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisboa, 1749-016 Lisboa, Portugal"},{"name":"3Center of Human Genetics, National Institute of Health, 1649-016 Lisboa, Portugal"}]},{"given":"Andreas","family":"Englmann","sequence":"additional","affiliation":[{"name":"1Ludwig Maximilians University Munich, Department of Biology II, 80336 Munich, Germany"}]},{"given":"Susanne","family":"Lang","sequence":"additional","affiliation":[{"name":"1Ludwig Maximilians University Munich, Department of Biology II, 80336 Munich, Germany"}]},{"given":"Luka A.","family":"Clarke","sequence":"additional","affiliation":[{"name":"2Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisboa, 1749-016 Lisboa, Portugal"}]},{"given":"Carsten","family":"Rudolph","sequence":"additional","affiliation":[{"name":"4Ludwig Maximilians University Munich, Division of Molecular Pulmonology, Department of Pediatrics, 80337 Munich, Germany"}]},{"given":"Felix","family":"Alt","sequence":"additional","affiliation":[{"name":"1Ludwig Maximilians University Munich, Department of Biology II, 80336 Munich, Germany"}]},{"given":"Kathrin","family":"Luther","sequence":"additional","affiliation":[{"name":"1Ludwig Maximilians University Munich, Department of Biology II, 80336 Munich, Germany"}]},{"given":"Carla","family":"Braz","sequence":"additional","affiliation":[{"name":"2Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisboa, 1749-016 Lisboa, Portugal"}]},{"given":"Nicolas","family":"Sadoni","sequence":"additional","affiliation":[{"name":"1Ludwig Maximilians University Munich, Department of Biology II, 80336 Munich, Germany"}]},{"given":"Joseph","family":"Rosenecker","sequence":"additional","affiliation":[{"name":"4Ludwig Maximilians University Munich, Division of Molecular Pulmonology, Department of Pediatrics, 80337 Munich, Germany"}]},{"given":"Dirk","family":"Schindelhauer","sequence":"additional","affiliation":[{"name":"5Technical University of Munich, Institute of Human Genetics, 81675 Munich, Germany"},{"name":"6Life Science Center Weihenstephan, 85354 Freising, Germany"}]}],"member":"291","published-online":{"date-parts":[[2004,9,13]]},"reference":[{"key":"2023072906401366700_BIB1","first-page":"17","volume":"3 Suppl 2","year":"2004","journal-title":"J. Cystic Fibrosis."},{"key":"2023072906401366700_BIB2","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1101\/gad.1102803","volume":"17","year":"2003","journal-title":"Genes Dev."},{"key":"2023072906401366700_BIB3","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/BF00291049","volume":"98","year":"1989","journal-title":"Chromosoma."},{"key":"2023072906401366700_BIB4","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1093\/hmg\/10.3.211","volume":"10","year":"2001","journal-title":"Hum. Mol. Genet."},{"key":"2023072906401366700_BIB5","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/S0092-8674(00)80472-9","volume":"91","year":"1997","journal-title":"Cell."},{"key":"2023072906401366700_BIB6","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S1097-2765(00)80311-1","volume":"3","year":"1999","journal-title":"Mol. Cell."},{"key":"2023072906401366700_BIB7","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1038\/35078577","volume":"3","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"2023072906401366700_BIB8","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1006\/geno.2001.6651","volume":"78","year":"2001","journal-title":"Genomics."},{"key":"2023072906401366700_BIB9","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1091\/mbc.10.1.211","volume":"10","year":"1999","journal-title":"Mol. Biol. Cell."},{"key":"2023072906401366700_BIB10","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1038\/35066075","volume":"2","year":"2001","journal-title":"Nat. Rev. Genet."},{"key":"2023072906401366700_BIB11","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1101\/SQB.1993.058.01.085","volume":"58","year":"1993","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"2023072906401366700_BIB12"},{"key":"2023072906401366700_BIB13","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1083\/jcb.145.6.1119","volume":"145","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072906401366700_BIB14","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1083\/jcb.135.6.1427","volume":"135","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072906401366700_BIB15","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1172\/JCI117028","volume":"93","year":"1994","journal-title":"J. Clin. Invest"},{"key":"2023072906401366700_BIB16","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/0962-8924(94)90180-5","volume":"4","year":"1994","journal-title":"Trends Cell Biol."},{"key":"2023072906401366700_BIB17","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1083\/jcb.139.7.1597","volume":"139","year":"1997","journal-title":"J. Cell Biol."},{"key":"2023072906401366700_BIB18","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1002\/j.1460-2075.1995.tb07151.x","volume":"14","year":"1995","journal-title":"EMBO J."},{"key":"2023072906401366700_BIB19","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/S0092-8674(04)00171-0","volume":"116","year":"2004","journal-title":"Cell."},{"key":"2023072906401366700_BIB20","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1126\/science.1068768","volume":"296","year":"2002","journal-title":"Science."},{"key":"2023072906401366700_BIB21","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/331358a0","volume":"331","year":"1988","journal-title":"Nature."},{"key":"2023072906401366700_BIB22","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1083\/jcb.200207115","volume":"159","year":"2002","journal-title":"J. Cell Biol."},{"key":"2023072906401366700_BIB23","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1083\/jcb.200111071","volume":"157","year":"2002","journal-title":"J. Cell Biol."},{"key":"2023072906401366700_BIB24","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/ng843","volume":"30","year":"2002","journal-title":"Nat. Genet."},{"key":"2023072906401366700_BIB25","doi-asserted-by":"crossref","first-page":"3297","DOI":"10.1242\/jcs.114.18.3297","volume":"114","year":"2001","journal-title":"J. Cell Sci."},{"key":"2023072906401366700_BIB26","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1083\/jcb.135.6.1685","volume":"135","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072906401366700_BIB27","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1083\/jcb.146.6.1211","volume":"146","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072906401366700_BIB28","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1023\/A:1026799818566","volume":"9","year":"2001","journal-title":"Chromosome Res."},{"key":"2023072906401366700_BIB29","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1101\/gad.14.8.940","volume":"14","year":"2000","journal-title":"Genes Dev."},{"key":"2023072906401366700_BIB30","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1038\/ni0901-848","volume":"2","year":"2001","journal-title":"Nat. Immunol."},{"key":"2023072906401366700_BIB31","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1006\/excr.2002.5513","volume":"276","year":"2002","journal-title":"Exp. Cell Res."},{"key":"2023072906401366700_BIB32","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1038\/35055033","volume":"3","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"2023072906401366700_BIB33","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1083\/jcb.147.1.13","volume":"147","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072906401366700_BIB34","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1242\/jcs.113.9.1565","volume":"113","year":"2000","journal-title":"J. Cell Sci."},{"key":"2023072906401366700_BIB35","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1006\/excr.2001.5400","volume":"272","year":"2002","journal-title":"Exp. Cell Res."},{"key":"2023072906401366700_BIB36","first-page":"1168","volume":"16","year":"2002","journal-title":"Mol. Endocrinol."},{"key":"2023072906401366700_BIB37","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s004390050686","volume":"102","year":"1998","journal-title":"Hum. Genet."},{"key":"2023072906401366700_BIB38","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/S1046-2023(02)00289-X","volume":"29","year":"2003","journal-title":"Methods."}],"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/166\/6\/815\/1870841\/jcb1666815.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/166\/6\/815\/1870841\/jcb1666815.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T06:41:16Z","timestamp":1690612876000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/166\/6\/815\/51282\/Transcription-dependent-spatial-arrangements-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,13]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2004,9,13]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.200404107","relation":{},"ISSN":["1540-8140","0021-9525"],"issn-type":[{"value":"1540-8140","type":"electronic"},{"value":"0021-9525","type":"print"}],"subject":[],"published":{"date-parts":[[2004,9,13]]}}}