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We show that a truncated CTD containing 31 repeats (heptads 1\u201323, 36\u201338, and 48\u201352) is sufficient to support transcription, splicing, cleavage, and polyadenylation. Yet, the resulting mRNAs are mostly retained in the vicinity of the gene after transcriptional shutoff. The retained mRNAs maintain the ability to recruit components of the exon junction complex and the nuclear exosome subunit Rrp6p, suggesting that binding of these proteins is not sufficient for RNA release. We propose that the missing heptads in the truncated CTD mutant are required for binding of proteins implicated in a final co-transcriptional maturation of spliced and 3\u2032 end cleaved and polyadenylated mRNAs into export-competent ribonucleoprotein particles.<\/jats:p>","DOI":"10.1083\/jcb.200612109","type":"journal-article","created":{"date-parts":[[2007,10,16]],"date-time":"2007-10-16T00:44:22Z","timestamp":1192495462000},"page":"199-207","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":29,"title":["Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site"],"prefix":"10.1083","volume":"179","author":[{"given":"Noe\u0301lia","family":"Custo\u0301dio","sequence":"first","affiliation":[{"name":"1Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Maria","family":"Vivo","sequence":"additional","affiliation":[{"name":"1Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Michael","family":"Antoniou","sequence":"additional","affiliation":[{"name":"2Nuclear Biology Group, Department of Medical and Molecular Genetics, King's College London School of Medicine, Guy's Campus, Guy's Hospital, London SE1 9RT, England, UK"}]},{"given":"Maria","family":"Carmo-Fonseca","sequence":"additional","affiliation":[{"name":"1Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]}],"member":"291","published-online":{"date-parts":[[2007,10,15]]},"reference":[{"key":"2023072909141704300_BIB1","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.ceb.2005.03.001","volume":"17","year":"2005","journal-title":"Curr. 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