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In this paper, we show that tension uniformity at metaphase kinetochores and subsequent anaphase synchrony in Drosophila S2 cells are promoted by spindle microtubule flux. These results can be explained by a mechanical model of the spindle where microtubule poleward translocation events associated with flux reflect relaxation of the kinetochore\u2013microtubule interface, which accounts for the redistribution and convergence of kinetochore tensions in a timescale comparable to typical metaphase duration. As predicted by the model, experimental acceleration of mitosis precludes tension equalization and anaphase synchrony. We propose that flux-dependent equalization of kinetochore tensions ensures a timely and uniform maturation of kinetochore\u2013microtubule interfaces necessary for error-free and coordinated segregation of chromosomes in anaphase.<\/jats:p>","DOI":"10.1083\/jcb.200904153","type":"journal-article","created":{"date-parts":[[2009,7,7]],"date-time":"2009-07-07T01:28:51Z","timestamp":1246930131000},"page":"11-26","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":86,"title":["Synchronizing chromosome segregation by flux-dependent force equalization at kinetochores"],"prefix":"10.1083","volume":"186","author":[{"given":"Irina","family":"Matos","sequence":"first","affiliation":[{"name":"Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal 1"}]},{"given":"Ant\u00f3nio J.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal 1"}]},{"given":"Mariana","family":"Lince-Faria","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal 1"}]},{"given":"Lisa A.","family":"Cameron","sequence":"additional","affiliation":[{"name":"Department of Biology, University of North Carolina, Chapel Hill, NC 27599 2"}]},{"given":"Edward D.","family":"Salmon","sequence":"additional","affiliation":[{"name":"Department of Biology, University of North Carolina, Chapel Hill, NC 27599 2"}]},{"given":"Helder","family":"Maiato","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal 1"},{"name":"Laboratory of Cell and Molecular Biology, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal 3"}]}],"member":"291","published-online":{"date-parts":[[2009,7,6]]},"reference":[{"key":"2023072918253770600_bib1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/ncb1809","article-title":"Genome stability is ensured by temporal control of kinetochore-microtubule dynamics","volume":"11","author":"Bakhoum","year":"2009","journal-title":"Nat. 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