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We established a cell-free system from individual Drosophila melanogaster embryos that supports successive nuclear division cycles with native characteristics. This allowed us to investigate nuclear separation in predefined volumes. Encapsulating nuclei in microchambers revealed that the early cytoplasm is programmed to separate nuclei a distinct distance. Laser microsurgery revealed an important role of microtubule aster migration through cytoplasmic space, which depended on F-actin and cooperated with anaphase spindle elongation. These activities define a characteristic separation length scale that appears to be a conserved property of developing insect embryos.<\/jats:p>","DOI":"10.1083\/jcb.201204019","type":"journal-article","created":{"date-parts":[[2012,6,19]],"date-time":"2012-06-19T02:56:07Z","timestamp":1340074567000},"page":"887-895","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":100,"title":["Aster migration determines the length scale of nuclear separation in the <i>Drosophila<\/i> syncytial embryo"],"prefix":"10.1083","volume":"197","author":[{"given":"Ivo A.","family":"Telley","sequence":"first","affiliation":[{"name":"Cell Biology and Biophysics Unit 1 and 2"},{"name":"Developmental Biology Unit, European Molecular Biology Laboratory, 69126 Heidelberg, Germany 1 and 2"},{"name":"Cell Biology and Biophysics Unit 1 and 2"},{"name":"Developmental Biology Unit, European Molecular Biology Laboratory, 69126 Heidelberg, Germany 1 and 2"}]},{"given":"Imre","family":"G\u00e1sp\u00e1r","sequence":"additional","affiliation":[{"name":"Cell Biology and Biophysics Unit 1 and 2"},{"name":"Developmental Biology Unit, European Molecular Biology Laboratory, 69126 Heidelberg, Germany 1 and 2"}]},{"given":"Anne","family":"Ephrussi","sequence":"additional","affiliation":[{"name":"Cell Biology and Biophysics Unit 1 and 2"},{"name":"Developmental Biology Unit, European Molecular Biology Laboratory, 69126 Heidelberg, Germany 1 and 2"}]},{"given":"Thomas","family":"Surrey","sequence":"additional","affiliation":[{"name":"Cell Biology and Biophysics Unit 1 and 2"},{"name":"Developmental Biology Unit, European Molecular Biology Laboratory, 69126 Heidelberg, Germany 1 and 2"},{"name":"Cancer Research UK London Research Institute, Lincoln\u2019s Inn Fields Laboratories, London WC2A 3LY, England, UK 3"}]}],"member":"291","published-online":{"date-parts":[[2012,6,18]]},"reference":[{"key":"2023072306173803700_bib1","first-page":"248","article-title":"The Embryology of Dacus tryoni (Frogg.) 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