{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:44:13Z","timestamp":1767962653297,"version":"3.49.0"},"reference-count":32,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,4,29]]},"abstract":"<jats:p>Most solid tumors contain aneuploid cells, indicating that the mitotic checkpoint is permissive to the proliferation of chromosomally aberrant cells. However, mutated or altered expression of mitotic checkpoint genes accounts for a minor proportion of human tumors. We describe a Drosophila melanogaster tumorigenesis model derived from knocking down spindle assembly checkpoint (SAC) genes and preventing apoptosis in wing imaginal discs. Bub3-deficient tumors that were also deficient in apoptosis displayed neoplastic growth, chromosomal aneuploidy, and high proliferative potential after transplantation into adult flies. Inducing aneuploidy by knocking down CENP-E and preventing apoptosis does not induce tumorigenesis, indicating that aneuploidy is not sufficient for hyperplasia. In this system, the aneuploidy caused by a deficient SAC is not driving tumorigenesis because preventing Bub3 from binding to the kinetochore does not cause hyperproliferation. Our data suggest that Bub3 has a nonkinetochore-dependent function that is consistent with its role as a tumor suppressor.<\/jats:p>","DOI":"10.1083\/jcb.201210018","type":"journal-article","created":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T06:18:25Z","timestamp":1366697905000},"page":"385-393","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":58,"title":["A tumor suppressor role of the Bub3 spindle checkpoint protein after apoptosis inhibition"],"prefix":"10.1083","volume":"201","author":[{"given":"Sara","family":"Morais da Silva","sequence":"first","affiliation":[{"name":"Instituto de Biologia Molecular e Celular 1 and 2"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar, Universidade do Porto, 4099 Porto, Portugal 1 and 2"}]},{"given":"Tatiana","family":"Moutinho-Santos","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular 1 and 2"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar, Universidade do Porto, 4099 Porto, Portugal 1 and 2"}]},{"given":"Claudio E.","family":"Sunkel","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular 1 and 2"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar, Universidade do Porto, 4099 Porto, Portugal 1 and 2"},{"name":"Instituto de Biologia Molecular e Celular 1 and 2"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar, Universidade do Porto, 4099 Porto, Portugal 1 and 2"}]}],"member":"291","published-online":{"date-parts":[[2013,4,22]]},"reference":[{"key":"2023072307140213900_bib1","first-page":"243","article-title":"Transplantation and in vivo culture","author":"Ashburner","year":"1989"},{"key":"2023072307140213900_bib2","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1083\/jcb.200211048","article-title":"Rae1 is an essential mitotic checkpoint regulator that cooperates with Bub3 to prevent chromosome missegregation","volume":"160","author":"Babu","year":"2003","journal-title":"J. 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