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We show that this actomyosin flow was regulated by Diaphanous and ROCK and that it elicited a wave of apical cell constriction that culminated in the formation of the leading edge actomyosin cable, a structure that is essential for wound closure. Calcium signaling played an important role in this process, as its intracellular concentration increased dramatically immediately after wounding, and down-regulation of transient receptor potential channel M, a stress-activated calcium channel, also impaired the actomyosin flow. Lowering the activity of Gelsolin, a known calcium-activated actin filament\u2013severing protein, also impaired the wound response, indicating that cleaving the existing actin filament network is an important part of the cytoskeleton remodeling process.<\/jats:p>","DOI":"10.1083\/jcb.201211039","type":"journal-article","created":{"date-parts":[[2013,7,22]],"date-time":"2013-07-22T15:56:35Z","timestamp":1374508595000},"page":"365-379","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":145,"title":["Coordinated waves of actomyosin flow and apical cell constriction immediately after wounding"],"prefix":"10.1083","volume":"202","author":[{"given":"Marco","family":"Antunes","sequence":"first","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal 1"},{"name":"Centro de Estudos de Doen\u00e7as Cr\u00f3nicas (CEDOC), Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Nova de Lisboa, 1169-056 Lisboa, Portugal 2"}]},{"given":"Telmo","family":"Pereira","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal 1"},{"name":"Centro de Estudos de Doen\u00e7as Cr\u00f3nicas (CEDOC), Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Nova de Lisboa, 1169-056 Lisboa, Portugal 2"}]},{"given":"Jo\u00e3o V.","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal 1"}]},{"given":"Luis","family":"Almeida","sequence":"additional","affiliation":[{"name":"Laboratoire Jacques-Louis Lions, Universit\u00e9 Pierre et Marie Curie and Centre National de la Recherche Scientifique, F75252 Paris, France 3"}]},{"given":"Antonio","family":"Jacinto","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal 1"},{"name":"Centro de Estudos de Doen\u00e7as Cr\u00f3nicas (CEDOC), Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Nova de Lisboa, 1169-056 Lisboa, Portugal 2"},{"name":"Instituto Gulbenkian de Ci\u00eancia, 2780-156 Oeiras, Portugal 4"}]}],"member":"291","published-online":{"date-parts":[[2013,7,22]]},"reference":[{"key":"2023072306002287500_bib1","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1002\/cm.20472","article-title":"Rho-kinase\/ROCK: A key regulator of the cytoskeleton and cell polarity","volume":"67","author":"Amano","year":"2010","journal-title":"Cytoskeleton (Hoboken)."},{"key":"2023072306002287500_bib2","first-page":"57","article-title":"Staging the metamorphosis of Drosophila melanogaster","volume":"66","author":"Bainbridge","year":"1981","journal-title":"J. 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