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Whereas EB1 was involved in spindle orientation before anaphase, EB3 was required for stabilization of focal adhesions and coordinated daughter cell spreading during mitotic exit. Additionally, EB3 promoted midbody microtubule stability and, consequently, midbody stabilization necessary for efficient cytokinesis. Importantly, daughter cell adhesion and cytokinesis completion were spatially regulated by distinct states of EB3 phosphorylation on serine 176 by Aurora B. This EB3 phosphorylation was enriched at the midbody and shown to control cortical microtubule growth. These findings uncover differential roles of EB proteins and explain the importance of an Aurora B phosphorylation gradient for the spatiotemporal regulation of microtubule function during mitotic exit and cytokinesis.<\/jats:p>","DOI":"10.1083\/jcb.201301131","type":"journal-article","created":{"date-parts":[[2013,5,27]],"date-time":"2013-05-27T11:42:54Z","timestamp":1369654974000},"page":"709-724","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":55,"title":["Aurora B spatially regulates EB3 phosphorylation to coordinate daughter cell adhesion with cytokinesis"],"prefix":"10.1083","volume":"201","author":[{"given":"Jorge G.","family":"Ferreira","sequence":"first","affiliation":[{"name":"Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular (IBMC) 1 , and 2"},{"name":"Cell Division Unit, Department of Experimental Biology, Faculty of Medicine, University of Porto, 4150-180 Porto, Portugal 1 , and 2"},{"name":"Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular (IBMC) 1 , and 2"},{"name":"Cell Division Unit, Department of Experimental Biology, Faculty of Medicine, University of Porto, 4150-180 Porto, Portugal 1 , and 2"}]},{"given":"Ant\u00f3nio J.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular (IBMC) 1 , and 2"},{"name":"Cell Division Unit, Department of Experimental Biology, Faculty of Medicine, University of Porto, 4150-180 Porto, Portugal 1 , and 2"}]},{"given":"Anna","family":"Akhmanova","sequence":"additional","affiliation":[{"name":"Cell Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, Netherlands 3"}]},{"given":"Helder","family":"Maiato","sequence":"additional","affiliation":[{"name":"Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular (IBMC) 1 , and 2"},{"name":"Cell Division Unit, Department of Experimental Biology, Faculty of Medicine, University of Porto, 4150-180 Porto, Portugal 1 , and 2"},{"name":"Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular (IBMC) 1 , and 2"},{"name":"Cell Division Unit, Department of Experimental Biology, Faculty of Medicine, University of Porto, 4150-180 Porto, Portugal 1 , and 2"}]}],"member":"291","published-online":{"date-parts":[[2013,5,27]]},"reference":[{"key":"2023072307115327000_bib1","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1038\/nrm2369","article-title":"Tracking the ends: a dynamic protein network controls the fate of microtubule tips","volume":"9","author":"Akhmanova","year":"2008","journal-title":"Nat. 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