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This mechanism relies on a membranous system surrounding the mitotic spindle that defines an organelle-exclusion zone that is conserved in human cells. Supported by mathematical modeling, we demonstrate that organelle exclusion by a membrane system causes spatio-temporal differences in molecular crowding states that are sufficient to drive accumulation of mitotic regulators, such as Mad2 and Megator\/Tpr, as well as soluble tubulin, in the spindle region. This membranous \u201cspindle envelope\u201d confined spindle assembly, and its mechanical disruption compromised faithful chromosome segregation. Thus, cytoplasmic compartmentalization persists during early mitosis to promote spindle assembly and function.<\/jats:p>","DOI":"10.1083\/jcb.201506107","type":"journal-article","created":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T21:10:42Z","timestamp":1440450642000},"page":"695-704","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":65,"title":["An organelle-exclusion envelope assists mitosis and underlies distinct molecular crowding in the spindle region"],"prefix":"10.1083","volume":"210","author":[{"given":"Nina","family":"Schweizer","sequence":"first","affiliation":[{"name":"Chromosome Instability & Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal 1"},{"name":"Cell Division Unit, Department of Experimental Biology, Faculdade de Medicina, Universidade do Porto, Alameda Prof. Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal 2"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (i3S), Universidade do Porto, Porto, Portugal 3"}]},{"given":"Nisha","family":"Pawar","sequence":"additional","affiliation":[{"name":"Experimental Physics I, University of Bayreuth, D-95440 Bayreuth, Germany 4"}]},{"given":"Matthias","family":"Weiss","sequence":"additional","affiliation":[{"name":"Experimental Physics I, University of Bayreuth, D-95440 Bayreuth, Germany 4"}]},{"given":"Helder","family":"Maiato","sequence":"additional","affiliation":[{"name":"Chromosome Instability & Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal 1"},{"name":"Cell Division Unit, Department of Experimental Biology, Faculdade de Medicina, Universidade do Porto, Alameda Prof. Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal 2"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (i3S), Universidade do Porto, Porto, Portugal 3"}]}],"member":"291","published-online":{"date-parts":[[2015,8,24]]},"reference":[{"key":"2023072309394861500_bib1","doi-asserted-by":"publisher","first-page":"17343","DOI":"10.1073\/pnas.1103720108","article-title":"Rab5 GTPase controls chromosome alignment through Lamin disassembly and relocation of the NuMA-like protein Mud to the poles during mitosis","volume":"108","author":"Capalbo","year":"2011","journal-title":"Proc. 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