{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T18:21:30Z","timestamp":1773339690645,"version":"3.50.1"},"reference-count":57,"publisher":"Rockefeller University Press","issue":"10","license":[{"start":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T00:00:00Z","timestamp":1574208000000},"content-version":"vor","delay-in-days":496,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Lisboa Regional Operational Program","award":["LISBOA-01-0145-FEDER-022170"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER-022170"]}]},{"name":"Lisboa Regional Operational Program","award":["PPBI-POCI-01-0145-FEDER-022122"],"award-info":[{"award-number":["PPBI-POCI-01-0145-FEDER-022122"]}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["LISBOA-01-0145-FEDER-022170"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER-022170"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["PPBI-POCI-01-0145-FEDER-022122"],"award-info":[{"award-number":["PPBI-POCI-01-0145-FEDER-022122"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["LISBOA-01-0145-FEDER-022170"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER-022170"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PPBI-POCI-01-0145-FEDER-022122"],"award-info":[{"award-number":["PPBI-POCI-01-0145-FEDER-022122"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["IF\/00851\/2012\/CP0185\/CT0004"],"award-info":[{"award-number":["IF\/00851\/2012\/CP0185\/CT0004"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004410","name":"European Molecular Biology Organization","doi-asserted-by":"publisher","award":["IG2778"],"award-info":[{"award-number":["IG2778"]}],"id":[{"id":"10.13039\/100004410","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"European Research Council","doi-asserted-by":"publisher","award":["ERC-2014-STG-638917"],"award-info":[{"award-number":["ERC-2014-STG-638917"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,10,1]]},"abstract":"<jats:p>Sister chromatid cohesion mediated by cohesin is essential for mitotic fidelity. It counteracts spindle forces to prevent premature chromatid individualization and random genome segregation. However, it is unclear what effects a partial decline of cohesin may have on chromosome organization. In this study, we provide a quantitative analysis of cohesin decay by inducing acute removal of defined amounts of cohesin from metaphase-arrested chromosomes. We demonstrate that sister chromatid cohesion is very resistant to cohesin loss as chromatid disjunction is only observed when chromosomes lose &amp;gt;80% of bound cohesin. Removal close to this threshold leads to chromosomes that are still cohered but display compromised chromosome alignment and unstable spindle attachments. Partial cohesin decay leads to increased duration of mitosis and susceptibility to errors in chromosome segregation. We propose that high cohesin density ensures centromeric chromatin rigidity necessary to maintain a force balance with the mitotic spindle. Partial cohesin loss may lead to chromosome segregation errors even when sister chromatid cohesion is fulfilled.<\/jats:p>","DOI":"10.1083\/jcb.201801111","type":"journal-article","created":{"date-parts":[[2018,7,12]],"date-time":"2018-07-12T17:25:37Z","timestamp":1531416337000},"page":"3343-3353","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":22,"title":["A quantitative analysis of cohesin decay in mitotic fidelity"],"prefix":"10.1083","volume":"217","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6004-3286","authenticated-orcid":true,"given":"Sara","family":"Carvalhal","sequence":"first","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5332-3386","authenticated-orcid":true,"given":"Alexandra","family":"Tavares","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6350-544X","authenticated-orcid":true,"given":"Mariana B.","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0802-7200","authenticated-orcid":true,"given":"Mihailo","family":"Mirkovic","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8293-8603","authenticated-orcid":true,"given":"Raquel A.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Oeiras, Portugal"}]}],"member":"291","published-online":{"date-parts":[[2018,7,12]]},"reference":[{"key":"2023072314300083400_bib1","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1038\/nmeth.1896","article-title":"OMERO: flexible, model-driven data management for experimental biology","volume":"9","author":"Allan","year":"2012","journal-title":"Nat. Methods."},{"key":"2023072314300083400_bib2","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1534\/genetics.115.178467","article-title":"Under Tension: Kinetochores and Basic Research","volume":"200","author":"Biggins","year":"2015","journal-title":"Genetics."},{"key":"2023072314300083400_bib3","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1016\/j.cub.2007.03.033","article-title":"Pericentric chromatin is an elastic component of the mitotic spindle","volume":"17","author":"Bouck","year":"2007","journal-title":"Curr. Biol."},{"key":"2023072314300083400_bib4","doi-asserted-by":"publisher","first-page":"856","DOI":"10.1016\/j.cub.2005.03.052","article-title":"Recruitment of Mad2 to the kinetochore requires the Rod\/Zw10 complex","volume":"15","author":"Buffin","year":"2005","journal-title":"Curr. Biol."},{"key":"2023072314300083400_bib5","doi-asserted-by":"publisher","first-page":"1018","DOI":"10.1016\/j.cub.2011.05.032","article-title":"Cohesion fatigue induces chromatid separation in cells delayed at metaphase","volume":"21","author":"Daum","year":"2011","journal-title":"Curr. Biol."},{"key":"2023072314300083400_bib6","doi-asserted-by":"publisher","first-page":"a026476","DOI":"10.1101\/cshperspect.a026476","article-title":"Cohesin Mutations in Cancer","volume":"6","author":"De Koninck","year":"2016","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"2023072314300083400_bib7","doi-asserted-by":"publisher","first-page":"8399","DOI":"10.1038\/ncomms9399","article-title":"Defective sister chromatid cohesion is synthetically lethal with impaired APC\/C function","volume":"6","author":"de Lange","year":"2015","journal-title":"Nat. Commun."},{"key":"2023072314300083400_bib8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00412-006-0072-6","article-title":"Roles of the sister chromatid cohesion apparatus in gene expression, development, and human syndromes","volume":"116","author":"Dorsett","year":"2007","journal-title":"Chromosoma."},{"key":"2023072314300083400_bib9","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/j.cub.2005.12.040","article-title":"Condensin I stabilizes chromosomes mechanically through a dynamic interaction in live cells","volume":"16","author":"Gerlich","year":"2006","journal-title":"Curr. Biol."},{"key":"2023072314300083400_bib10","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/S0092-8674(01)80008-8","article-title":"A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae","volume":"91","author":"Guacci","year":"1997","journal-title":"Cell."},{"key":"2023072314300083400_bib11","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1038\/nature07098","article-title":"The cohesin ring concatenates sister DNA molecules","volume":"454","author":"Haering","year":"2008","journal-title":"Nature."},{"key":"2023072314300083400_bib12","doi-asserted-by":"publisher","first-page":"957","DOI":"10.1016\/j.cub.2010.04.018","article-title":"Systematic reduction of cohesin differentially affects chromosome segregation, condensation, and DNA repair","volume":"20","author":"Heidinger-Pauli","year":"2010","journal-title":"Curr. Biol."},{"key":"2023072314300083400_bib13","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1007\/s00412-004-0305-5","article-title":"The Drosophila meiotic kleisin C(2)M functions before the meiotic divisions","volume":"113","author":"Heidmann","year":"2004","journal-title":"Chromosoma."},{"key":"2023072314300083400_bib14","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1007\/BF00344159","article-title":"Studies of mitotic and centromeric abnormalities in Roberts syndrome: implications for a defect in the mitotic mechanism","volume":"100","author":"Jabs","year":"1991","journal-title":"Chromosoma."},{"key":"2023072314300083400_bib15","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1083\/jcb.201502046","article-title":"DNA loops generate intracentromere tension in mitosis","volume":"210","author":"Lawrimore","year":"2015","journal-title":"J. Cell Biol."},{"key":"2023072314300083400_bib16","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1146\/annurev.genom.9.081307.164211","article-title":"Cohesin and human disease","volume":"9","author":"Liu","year":"2008","journal-title":"Annu. Rev. Genomics Hum. Genet."},{"key":"2023072314300083400_bib17","doi-asserted-by":"publisher","first-page":"1757","DOI":"10.1242\/jcs.01033","article-title":"Different spindle checkpoint proteins monitor microtubule attachment and tension at kinetochores in Drosophila cells","volume":"117","author":"Logarinho","year":"2004","journal-title":"J. Cell Sci."},{"key":"2023072314300083400_bib18","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1038\/nrc3743","article-title":"Cohesin in cancer: chromosome segregation and beyond","volume":"14","author":"Losada","year":"2014","journal-title":"Nat. Rev. Cancer."},{"key":"2023072314300083400_bib19","doi-asserted-by":"publisher","first-page":"1986","DOI":"10.1101\/gad.12.13.1986","article-title":"Identification of Xenopus SMC protein complexes required for sister chromatid cohesion","volume":"12","author":"Losada","year":"1998","journal-title":"Genes Dev."},{"key":"2023072314300083400_bib20","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1083\/jcb.200808130","article-title":"Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity","volume":"184","author":"Maresca","year":"2009","journal-title":"J. Cell Biol."},{"key":"2023072314300083400_bib21","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1242\/jcs.064790","article-title":"Welcome to a new kind of tension: translating kinetochore mechanics into a wait-anaphase signal","volume":"123","author":"Maresca","year":"2010","journal-title":"J. Cell Sci."},{"key":"2023072314300083400_bib22","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1083\/jcb.200904153","article-title":"Synchronizing chromosome segregation by flux-dependent force equalization at kinetochores","volume":"186","author":"Matos","year":"2009","journal-title":"J. Cell Biol."},{"key":"2023072314300083400_bib23","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/S0092-8674(01)80007-6","article-title":"Cohesins: chromosomal proteins that prevent premature separation of sister chromatids","volume":"91","author":"Michaelis","year":"1997","journal-title":"Cell."},{"key":"2023072314300083400_bib24","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1007\/978-3-319-58592-5_20","article-title":"Centromeric Cohesin: Molecular Glue and Much More","volume":"56","author":"Mirkovic","year":"2017","journal-title":"Prog. Mol. Subcell. Biol."},{"key":"2023072314300083400_bib25","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/j.celrep.2015.09.020","article-title":"Premature Sister Chromatid Separation Is Poorly Detected by the Spindle Assembly Checkpoint as a Result of System-Level Feedback","volume":"13","author":"Mirkovic","year":"2015","journal-title":"Cell Reports."},{"key":"2023072314300083400_bib26","doi-asserted-by":"publisher","first-page":"e1004492","DOI":"10.1371\/journal.pgen.1004492","article-title":"Tethering sister centromeres to each other suggests the spindle checkpoint detects stretch within the kinetochore","volume":"10","author":"Nannas","year":"2014","journal-title":"PLoS Genet."},{"key":"2023072314300083400_bib27","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1083\/jcb.97.2.542","article-title":"Measurements of the force produced by the mitotic spindle in anaphase","volume":"97","author":"Nicklas","year":"1983","journal-title":"J. Cell Biol."},{"key":"2023072314300083400_bib28","doi-asserted-by":"publisher","first-page":"1639","DOI":"10.1042\/BST0381639","article-title":"Getting through anaphase: splitting the sisters and beyond","volume":"38","author":"Oliveira","year":"2010","journal-title":"Biochem. Soc. Trans."},{"key":"2023072314300083400_bib29","doi-asserted-by":"publisher","first-page":"8971","DOI":"10.1128\/MCB.25.20.8971-8984.2005","article-title":"The condensin I subunit Barren\/CAP-H is essential for the structural integrity of centromeric heterochromatin during mitosis","volume":"25","author":"Oliveira","year":"2005","journal-title":"Mol. Cell. Biol."},{"key":"2023072314300083400_bib30","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1038\/ncb2018","article-title":"Cohesin cleavage and Cdk inhibition trigger formation of daughter nuclei","volume":"12","author":"Oliveira","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"2023072314300083400_bib31","doi-asserted-by":"publisher","first-page":"e1001962","DOI":"10.1371\/journal.pbio.1001962","article-title":"Centromere-independent accumulation of cohesin at ectopic heterochromatin sites induces chromosome stretching during anaphase","volume":"12","author":"Oliveira","year":"2014","journal-title":"PLoS Biol."},{"key":"2023072314300083400_bib32","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1242\/bio.016394","article-title":"Unique geometry of sister kinetochores in human oocytes during meiosis I may explain maternal age-associated increases in chromosomal abnormalities","volume":"5","author":"Patel","year":"2015","journal-title":"Biol. Open."},{"key":"2023072314300083400_bib33","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/j.devcel.2007.12.009","article-title":"Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons","volume":"14","author":"Pauli","year":"2008","journal-title":"Dev. Cell."},{"key":"2023072314300083400_bib34","doi-asserted-by":"publisher","first-page":"e26120","DOI":"10.7554\/eLife.26120","article-title":"Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation","volume":"6","author":"Piskadlo","year":"2017","journal-title":"eLife."},{"key":"2023072314300083400_bib35","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.1459","article-title":"Protein Extraction from Drosophila Embryos and Ovaries","volume":"5","author":"Prud\u00eancio","year":"2015","journal-title":"Bio Protoc."},{"key":"2023072314300083400_bib36","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.cell.2005.10.032","article-title":"The processivity of multiubiquitination by the APC determines the order of substrate degradation","volume":"124","author":"Rape","year":"2006","journal-title":"Cell."},{"key":"2023072314300083400_bib37","doi-asserted-by":"publisher","first-page":"3715","DOI":"10.1242\/dev.090605","article-title":"Cohesin in development and disease","volume":"140","author":"Remeseiro","year":"2013","journal-title":"Development."},{"key":"2023072314300083400_bib38","doi-asserted-by":"publisher","first-page":"2371","DOI":"10.1091\/mbc.e08-11-1127","article-title":"Condensin regulates the stiffness of vertebrate centromeres","volume":"20","author":"Ribeiro","year":"2009","journal-title":"Mol. Biol. Cell."},{"key":"2023072314300083400_bib39","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods."},{"key":"2023072314300083400_bib40","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1007\/s00412-007-0103-y","article-title":"Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes","volume":"116","author":"Schittenhelm","year":"2007","journal-title":"Chromosoma."},{"key":"2023072314300083400_bib41","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.cub.2006.11.051","article-title":"Incorporation of Drosophila CID\/CENP-A and CENP-C into centromeres during early embryonic anaphase","volume":"17","author":"Schuh","year":"2007","journal-title":"Curr. Biol."},{"key":"2023072314300083400_bib42","doi-asserted-by":"publisher","first-page":"1167","DOI":"10.1083\/jcb.201103138","article-title":"Cohesin, condensin, and the intramolecular centromere loop together generate the mitotic chromatin spring","volume":"193","author":"Stephens","year":"2011","journal-title":"J. Cell Biol."},{"key":"2023072314300083400_bib43","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1083\/jcb.201208163","article-title":"Pericentric chromatin loops function as a nonlinear spring in mitotic force balance","volume":"200","author":"Stephens","year":"2013","journal-title":"J. Cell Biol."},{"key":"2023072314300083400_bib44","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1038\/35019529","article-title":"Cohesin ensures bipolar attachment of microtubules to sister centromeres and resists their precocious separation","volume":"2","author":"Tanaka","year":"2000","journal-title":"Nat. Cell Biol."},{"key":"2023072314300083400_bib45","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1002\/ajmg.1320040104","article-title":"Cytogenetic findings in Roberts-SC phocomelia syndrome(s)","volume":"4","author":"Tomkins","year":"1979","journal-title":"Am. J. Med. Genet."},{"key":"2023072314300083400_bib46","doi-asserted-by":"publisher","first-page":"e96710","DOI":"10.1371\/journal.pone.0096710","article-title":"Age-related decrease of meiotic cohesins in human oocytes","volume":"9","author":"Tsutsumi","year":"2014","journal-title":"PLoS One."},{"key":"2023072314300083400_bib47","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1083\/jcb.200811028","article-title":"Kinetochore stretching inactivates the spindle assembly checkpoint","volume":"184","author":"Uchida","year":"2009","journal-title":"J. Cell Biol."},{"key":"2023072314300083400_bib48","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1038\/21831","article-title":"Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1","volume":"400","author":"Uhlmann","year":"1999","journal-title":"Nature."},{"key":"2023072314300083400_bib49","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/S0092-8674(00)00130-6","article-title":"Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast","volume":"103","author":"Uhlmann","year":"2000","journal-title":"Cell."},{"key":"2023072314300083400_bib50","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1016\/j.ajhg.2010.01.008","article-title":"Warsaw breakage syndrome, a cohesinopathy associated with mutations in the XPD helicase family member DDX11\/ChlR1","volume":"86","author":"van der Lelij","year":"2010","journal-title":"Am. J. Hum. Genet."},{"key":"2023072314300083400_bib51","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/S0092-8674(00)00132-X","article-title":"Two distinct pathways remove mammalian cohesin from chromosome arms in prophase and from centromeres in anaphase","volume":"103","author":"Waizenegger","year":"2000","journal-title":"Cell."},{"key":"2023072314300083400_bib52","doi-asserted-by":"publisher","first-page":"1463","DOI":"10.1016\/S0960-9822(00)00806-X","article-title":"The Drosophila RAD21 cohesin persists at the centromere region in mitosis","volume":"10","author":"Warren","year":"2000","journal-title":"Curr. Biol."},{"key":"2023072314300083400_bib53","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.tcb.2016.09.002","article-title":"Mechanisms of Aneuploidy in Human Eggs","volume":"27","author":"Webster","year":"2016","journal-title":"Trends Cell Biol."},{"key":"2023072314300083400_bib54","doi-asserted-by":"publisher","first-page":"13221","DOI":"10.1038\/ncomms13221","article-title":"Chromosome biorientation produces hundreds of piconewtons at a metazoan kinetochore","volume":"7","author":"Ye","year":"2016","journal-title":"Nat. Commun."},{"key":"2023072314300083400_bib55","doi-asserted-by":"publisher","first-page":"R144","DOI":"10.1016\/j.cub.2014.01.023","article-title":"Illuminating DNA replication during Drosophila development using TALE-lights","volume":"24","author":"Yuan","year":"2014","journal-title":"Curr. Biol."},{"key":"2023072314300083400_bib56","doi-asserted-by":"publisher","first-page":"1019","DOI":"10.1083\/jcb.200801157","article-title":"A handcuff model for the cohesin complex","volume":"183","author":"Zhang","year":"2008","journal-title":"J. Cell Biol."},{"key":"2023072314300083400_bib57","doi-asserted-by":"publisher","first-page":"e11389","DOI":"10.7554\/eLife.11389","article-title":"Sister kinetochore splitting and precocious disintegration of bivalents could explain the maternal age effect","volume":"4","author":"Zielinska","year":"2015","journal-title":"eLife."}],"container-title":["Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/217\/10\/3343\/1605523\/jcb_201801111.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/217\/10\/3343\/1605523\/jcb_201801111.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,23]],"date-time":"2023-07-23T14:30:47Z","timestamp":1690122647000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/217\/10\/3343\/120267\/A-quantitative-analysis-of-cohesin-decay-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,12]]},"references-count":57,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2018,10,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.201801111","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,12]]}}}