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Cell Biol."],"abstract":"<jats:p>CLASPs are conserved microtubule plus-end\u2013tracking proteins that suppress microtubule catastrophes and independently localize to kinetochores during mitosis. Thus, CLASPs are ideally positioned to regulate kinetochore\u2013microtubule dynamics required for chromosome segregation fidelity, but the underlying mechanism remains unknown. Here, we found that human CLASP2 exists predominantly as a monomer in solution, but it can self-associate through its C-terminal kinetochore-binding domain. Kinetochore localization was independent of self-association, and driving monomeric CLASP2 to kinetochores fully rescued normal kinetochore\u2013microtubule dynamics, while partially sustaining mitosis. CLASP2 kinetochore localization, recognition of growing microtubule plus-ends through EB\u2013protein interaction, and the ability to associate with curved microtubule protofilaments through TOG2 and TOG3 domains independently sustained normal spindle length, timely spindle assembly checkpoint satisfaction, chromosome congression, and faithful segregation. Measurements of kinetochore\u2013microtubule half-life and poleward flux revealed that CLASP2 regulates kinetochore\u2013microtubule dynamics by integrating distinctive microtubule-binding properties at the kinetochore\u2013microtubule interface. We propose that kinetochore CLASP2 suppresses microtubule depolymerization and detachment by binding to curved protofilaments at microtubule plus-ends.<\/jats:p>","DOI":"10.1083\/jcb.201905080","type":"journal-article","created":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T15:15:12Z","timestamp":1574435712000},"page":"jcb.201905080","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":14,"title":["CLASP2 binding to curved microtubule tips promotes flux and stabilizes kinetochore attachments"],"prefix":"10.1083","author":[{"given":"Hugo","family":"Gir\u00e3o","sequence":"first","affiliation":[{"name":"Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (i3S), Universidade do Porto, Porto, 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