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Mechanistic insight for nuclear positioning beyond cytoskeletal requirements is missing. Contemporary hypotheses propose actomyosin-driven cytoplasmic movement transporting nuclei or repulsion of neighbor nuclei driven by microtubule motors. Here, we show that microtubule cross-linking by Feo and Klp3A is essential for nuclear distribution and internuclear distance maintenance in Drosophila. Germline knockdown causes irregular, less-dense nuclear delivery to the cell cortex and smaller distribution in ex vivo embryo explants. A minimal internuclear distance is maintained in explants from control embryos but not from Feo-inhibited embryos, following micromanipulation-assisted repositioning. A dimerization-deficient Feo abolishes nuclear separation in embryo explants, while the full-length protein rescues the genetic knockdown. We conclude that Feo and Klp3A cross-linking of antiparallel microtubule overlap generates a length-regulated mechanical link between neighboring microtubule asters. Enabled by a novel experimental approach, our study illuminates an essential process of embryonic multicellularity.<\/jats:p>","DOI":"10.1083\/jcb.202007209","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T02:22:22Z","timestamp":1634782942000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":24,"title":["Astral microtubule cross-linking safeguards uniform nuclear distribution in the\n                    <i>Drosophila<\/i>\n                    syncytium"],"prefix":"10.1083","volume":"221","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8589-7790","authenticated-orcid":true,"given":"Ojas","family":"Deshpande","sequence":"first","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Funda\u00e7\u00e3o Calouste Gulbenkian, Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7429-4193","authenticated-orcid":true,"given":"Jorge","family":"de-Carvalho","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Funda\u00e7\u00e3o Calouste Gulbenkian, Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7388-6904","authenticated-orcid":true,"given":"Diana V.","family":"Vieira","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Funda\u00e7\u00e3o Calouste Gulbenkian, Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4444-1046","authenticated-orcid":true,"given":"Ivo A.","family":"Telley","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Funda\u00e7\u00e3o Calouste Gulbenkian, Oeiras, Portugal"}]}],"member":"291","published-online":{"date-parts":[[2021,11,12]]},"reference":[{"key":"2023072715542803900_bib1","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.devcel.2019.09.010","article-title":"Active Fluctuations of the Nuclear Envelope Shape the Transcriptional Dynamics in Oocytes","volume":"51","author":"Almonacid","year":"2019","journal-title":"Dev. 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