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At mid-oogenesis, Par-1 accumulates at the oocyte posterior end, while Par-3\/Bazooka is excluded there but maintains its localization along the remaining oocyte cortex. Past studies have proposed the need for somatic cells at the posterior end to initiate oocyte polarization by providing a trigger signal. To date, neither the molecular identity nor the nature of the signal is known. Here, we provide evidence that mechanical contact of posterior follicle cells (PFCs) with the oocyte cortex causes the posterior exclusion of Bazooka and maintains oocyte polarity. We show that Bazooka prematurely accumulates exclusively where posterior follicle cells have been mechanically detached or ablated. Furthermore, we provide evidence that PFC contact maintains Par-1 and oskar mRNA localization and microtubule cytoskeleton polarity in the oocyte. Our observations suggest that cell\u2013cell contact mechanics modulates Par protein binding sites at the oocyte cortex.<\/jats:p>","DOI":"10.1083\/jcb.202209052","type":"journal-article","created":{"date-parts":[[2022,11,5]],"date-time":"2022-11-05T02:21:13Z","timestamp":1667614873000},"update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":9,"title":["Follicle cell contact maintains main body axis polarity in the\n                    <i>Drosophila melanogaster<\/i>\n                    oocyte"],"prefix":"10.1083","volume":"222","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9184-8247","authenticated-orcid":true,"given":"Ana","family":"Milas","sequence":"first","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Funda\u00e7\u00e3o Calouste Gulbenkian, Rua da Quinta Grande, Portugal 1"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7429-4193","authenticated-orcid":true,"given":"Jorge","family":"de-Carvalho","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Funda\u00e7\u00e3o Calouste Gulbenkian, Rua da Quinta Grande, Portugal 1"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4444-1046","authenticated-orcid":true,"given":"Ivo A.","family":"Telley","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Funda\u00e7\u00e3o Calouste Gulbenkian, Rua da Quinta Grande, Portugal 1"}]}],"member":"291","published-online":{"date-parts":[[2022,11,21]]},"reference":[{"key":"2022112114011438900_bib1","doi-asserted-by":"publisher","first-page":"2819","DOI":"10.1242\/jcs.114.15.2819","article-title":"Specific domains drive VM32E protein distribution and integration in Drosophila eggshell layers","volume":"114","author":"Andrenacci","year":"2001","journal-title":"J. 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