{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T10:16:10Z","timestamp":1776420970950,"version":"3.51.2"},"reference-count":59,"publisher":"Rockefeller University Press","issue":"7","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,4,7]]},"abstract":"<jats:p>We have analyzed transgenic mice carrying versions of a human T cell receptor (TCR)-\u03b4 gene minilocus to study the developmental control of \u2009VDJ (variable\/diversity\/joining) recombination. Previous data indicated that a 1.4-kb DNA fragment carrying the TCR-\u03b4 enhancer (E\u03b4) efficiently activates minilocus VDJ recombination in vivo. We tested whether the transcription factor CBF\/PEBP2 plays an important role in the ability of E\u03b4 to activate VDJ recombination by analyzing VDJ recombination in mice carrying a minilocus in which the \u03b4E3 element of E\u03b4 includes a mutated CBF\/PEBP2 binding site. The enhancer-dependent VD to J step of minilocus rearrangement was dramatically inhibited in three of four transgenic lines, arguing that the binding of CBF\/PEBP2 plays a role in modulating local accessibility to the VDJ recombinase in vivo. Because mutation of the \u03b4E3 binding site for the transcription factor c-Myb had previously established a similar role for c-Myb, and because a 60-bp fragment of E\u03b4 carrying \u03b4E3 and \u03b4E4 binding sites for CBF\/PEBP2, c-Myb, and GATA-3 displays significant enhancer activity in transient transfection experiments, we tested whether this fragment of E\u03b4 is sufficient to activate VDJ recombination in vivo. This fragment failed to efficiently activate the enhancerdependent VD to J step of minilocus rearrangement in all three transgenic lines examined, indicating that the binding of CBF\/PEBP2 and c-Myb to their cognate sites within E\u03b4, although necessary, is not sufficient for the activation of VDJ recombination by E\u03b4. These results imply that CBF\/PEBP2 and c-Myb collaborate with additional factors that bind elsewhere within E\u03b4 to modulate local accessibility to the VDJ recombinase in vivo.<\/jats:p>","DOI":"10.1084\/jem.185.7.1193","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1193-1202","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":27,"title":["Regulation of \u2009T Cell Receptor \u03b4 Gene Rearrangement by CBF\/PEBP2"],"prefix":"10.1084","volume":"185","author":[{"given":"Pilar","family":"Lauzurica","sequence":"first","affiliation":[{"name":"From the Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Ping","family":"Zhong","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, Duke University Medical Center, 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