{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:48:05Z","timestamp":1761511685925},"reference-count":70,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,9,21]]},"abstract":"<jats:p>A high proportion of tumors arise due to mutation of the p53 tumor suppressor protein. A p53 hotspot mutation at amino acid position 273 from R to H, flanking a peptide epitope that spans residues 264\u2013272, renders cells resistant to killing by human histocompatibility leukocyte antigen (HLA)-A*0201\u2013restricted cytotoxic T lymphocytes (CTLs) specific for this epitope. Acquisition of the R to H mutation at residue 273 of the human p53 protein promotes tumor growth in vivo by selective escape from recognition by p53.264\u2013272 peptide-specific CTLs. Synthetic 27-mer p53 polypeptides covering the antigenic nonamer region 264\u2013272 of p53 were used as proteasome substrates to investigate whether the R to H mutation at the P1\u2032 position of the COOH terminus of the epitope affects proteasome-mediated processing of the protein. Analysis of the generated products by tandem mass spectrometry and the kinetics of polypeptide processing in conjunction with CTL assays demonstrate that the R to H mutation alters proteasomal processing of the p53 protein by inhibiting proteolytic cleavage between residues 272 and 273. This prevents the release of the natural CTL epitope that spans flanking residues 264\u2013272 as well as a putative precursor peptide. These results demonstrate that mutation of p53 not only leads to malignant transformation but may also, in some instances, affect immune surveillance and should be considered in the design of cancer vaccines.<\/jats:p>","DOI":"10.1084\/jem.188.6.1017","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1017-1028","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":87,"title":["The Sequence Alteration Associated with a Mutational Hotspot in p53 Protects Cells From Lysis by Cytotoxic T Lymphocytes Specific for a Flanking Peptide Epitope"],"prefix":"10.1084","volume":"188","author":[{"given":"Matthias","family":"Theobald","sequence":"first","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Thomas","family":"Ruppert","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Ulrike","family":"Kuckelkorn","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Javier","family":"Hernandez","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Annett","family":"H\u00e4ussler","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Edite Antunes","family":"Ferreira","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Ulrike","family":"Liewer","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Judith","family":"Biggs","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Arnold J.","family":"Levine","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Christoph","family":"Huber","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Ulrich H.","family":"Koszinowski","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Peter-M.","family":"Kloetzel","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]},{"given":"Linda A.","family":"Sherman","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology, Johannes Gutenberg-University, D-55101 Mainz, Germany; the \u2021Max von Pettenkofer-Institute for Virology, Ludwig Maximilians University, D-80336 Munich, Germany; the \u00a7Institute for Biochemistry, Medical Faculty (Charite), Humboldt University, D-10117 Berlin, Germany, the \u2016Department of Immunology, The Scripps Research Institute, La Jolla, California 92037; and the \u00b6Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544"}]}],"member":"291","published-online":{"date-parts":[[1998,9,21]]},"reference":[{"key":"2023072508173753800_B1","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/351453a0","article-title":"The p53 tumor suppressor 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