{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:23:07Z","timestamp":1778718187734,"version":"3.51.4"},"reference-count":36,"publisher":"Rockefeller University Press","issue":"8","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,10,17]]},"abstract":"<jats:p>T cell receptor (TCR) microclusters form within seconds of T cell contact with supported planar bilayers containing intercellular adhesion molecule-1 and agonist major histocompatibility complex (MHC)\u2013peptide complexes, and elevation of cytoplasmic Ca2+ is observed within seconds of the first detectable microclusters. At 0\u201330 s after contact, TCR microclusters are colocalized with activated forms of Lck, ZAP-70, and the linker for activation of T cells. By 2 min, activated kinases are reduced in the older central microclusters, but are abundant in younger peripheral microclusters. By 5 min, TCR in the central supramolecular activation cluster have reduced activated kinases, whereas faint peripheral TCR microclusters efficiently generated activated Lck and ZAP-70. TCR microcluster formation is resistant to inhibition by Src family kinase inhibitor PP2, but is abrogated by actin polymerization inhibitor latrunculin A. We propose that Src kinase\u2013independent formation of TCR microclusters in response to agonist MHC\u2013peptide provides an actin-dependent scaffold for signal amplification.<\/jats:p>","DOI":"10.1084\/jem.20051182","type":"journal-article","created":{"date-parts":[[2005,10,11]],"date-time":"2005-10-11T00:24:58Z","timestamp":1128990298000},"page":"1031-1036","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":538,"title":["Actin and agonist MHC\u2013peptide complex\u2013dependent T cell receptor microclusters as scaffolds for signaling"],"prefix":"10.1084","volume":"202","author":[{"given":"Gabriele","family":"Campi","sequence":"first","affiliation":[{"name":"Department of Pathology and the Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, New York, NY 10016"}]},{"given":"Rajat","family":"Varma","sequence":"additional","affiliation":[{"name":"Department of Pathology and the Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, New York, NY 10016"}]},{"given":"Michael L.","family":"Dustin","sequence":"additional","affiliation":[{"name":"Department of Pathology and the Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, New York, NY 10016"}]}],"member":"291","published-online":{"date-parts":[[2005,10,10]]},"reference":[{"key":"2023072513183309800_BIB1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/ni1173","volume":"6","year":"2005","journal-title":"Nat. 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