{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T02:00:23Z","timestamp":1785463223948,"version":"3.56.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1969,2,1]]},"abstract":"<jats:p>The principal results of the present study are: (a) the failure to find an antibody subset that binds a cross-reacting ligand better than the comparable homologue in spite of the use of isolation methods that select for such antibody molecules; (b) the isolation of antibody subsets with virtually indistinguishable average intrinsic association constants for homologous and cross-reacting ligands, but which nevertheless have physical properties (Qmax and relative fluorescence coefficient) that readily distinguish these subsets according to their origin in response to antigenic stimulation with DNP- or with TNP-protein; (c) the demonstration, by precipitin reaction and measurement of association constants for homologous and cross-reacting haptens, of generally greater cross-reactivity among high affinity anti-DNP and anti-TNP antibodies, i.e. low affinity antibodies are generally more discriminating; (d) the selection of anti-DNP and anti-TNP antibody subsets that are distinctive in their ability to show spur formation in gel diffusion reactions with homologous and cross-reacting antigens.<\/jats:p>\n               <jats:p>These results suggest that in the initial cellular response to antigenic stimulation, DNP-B\u03b3G and TNP-B\u03b3G stimulate virtually nonoverlapping sets of antigen-sensitive cells, despite the great similarity of these two immunogens. With prolonged stimulation this specificity wanes, giving rise to a more degenerate response evident in the greater cross-reactivity of the antibodies produced later in immunization.<\/jats:p>","DOI":"10.1084\/jem.129.2.247","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T23:23:31Z","timestamp":1088033011000},"page":"247-265","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":39,"title":["SPECIFICITY OF THE IMMUNE RESPONSE TO THE 2, 4-DINITROPHENYL AND 2, 4, 6-TRINITROPHENYL GROUPS"],"prefix":"10.1084","volume":"129","author":[{"given":"J. Russell","family":"Little","sequence":"first","affiliation":[{"name":"From the Department of Microbiology, Washington University School of Medicine, St. Louis, Missouri, 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herman N.","family":"Eisen","sequence":"additional","affiliation":[{"name":"From the Department of Microbiology, Washington University School of Medicine, St. Louis, Missouri, 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1969,2,1]]},"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/129\/2\/247\/1652990\/247.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/129\/2\/247\/1652990\/247.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T20:27:43Z","timestamp":1690230463000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/129\/2\/247\/5707\/SPECIFICITY-OF-THE-IMMUNE-RESPONSE-TO-THE-2-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1969,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1969,2,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.129.2.247","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[1969,2,1]]}}}