{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T17:01:12Z","timestamp":1760979672794},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1969,5,1]]},"abstract":"Further evidence implicating murine leukemia-like virus in the disorders of NZB mice was afforded by a study of antigens associated with murine leukemia virus (MuLV). MuLV group antigens were prevalent in extracts of spleen, kidney, and, to a lesser extent, thymus throughout a substantial portion of the life span of NZB mice as well as in extracts of lymphomas and sarcomas indigenous to the strain.<\/jats:p>\n G (Gross) soluble antigen, type-specific antigen, was first detected in plasma of untreated NZB mice at 3 months of age. G soluble antigen production increased thereafter in line with age, with 50% of reactions becoming positive at 5.3 months and 100% at 7 to 9 months.<\/jats:p>\n From months 3 to 9, the time-response curve for positive conversion of direct antiglobulin (Coombs) tests in untreated NZB mice corresponded closely to that for G soluble antigen production.<\/jats:p>\n Beyond the 9th month, G soluble antigen underwent elimination from the plasma of NZB mice, with positive reactions reduced to 50% at 13.3 months and to 0% at 18 months. G natural antibody was first detected in the serum of NZB mice at about 10 months of age and increased thereafter in line with age. The curves for G antibody production and G soluble antigen elimination bore a reciprocal relation to each other with crossover at 50% response occurring at 13.3 months.<\/jats:p>\n Significant proteinuria, a functional manifestation of membranous glomerulonephritis, became increasingly prevalent in female NZB mice as G soluble antigen was eliminated from plasma. Cumulative mortality of female NZB mice, mainly attributable to renal glomerular disease, increased in phase with G antibody production. MuLV group antigens were identified in the glomerular lesions by the immunofluorescence method.<\/jats:p>\n Positive conversion of direct antiglobulin tests was significantly delayed by vaccinating baby NZB mice with formaldehyde-inactivated cell-free filtrates of older NZB mouse spleens. The plasmas of vaccinated NZB mice with negative direct antiglobulin reactions at 4 to 7 months were likewise negative when tested for G soluble antigen. The 50% response time for G antibody production in the vaccinated NZB mice occurred at 7.3 months, that is, 6 months earlier than in untreated NZB mice.<\/jats:p>\n The collective findings implicate murine leukemia-like virus in the etiology of autoimmune hemolytic disease and membranous glomerulonephritis, as well as malignant lymphoma, of NZB mice and suggest that virus-specified cell-surface and soluble antigen is a factor in the immunopathogenesis of the renal disease and possibly also the autoimmune hemolytic disease.<\/jats:p>","DOI":"10.1084\/jem.129.5.1045","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T23:23:31Z","timestamp":1088033011000},"page":"1045-1062","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":112,"title":["FURTHER IMPLICATION OF MURINE LEUKEMIA-LIKE VIRUS IN THE DISORDERS OF NZB MICE"],"prefix":"10.1084","volume":"129","author":[{"given":"Robert C.","family":"Mellors","sequence":"first","affiliation":[{"name":"From The Hospital for Special Surgery and The Philip D. Wilson Research Foundation, Affiliated with The New York Hospital-Cornell University Medical College, and the Department of Pathology, Cornell University Medical College, New York 10021; the Division of Immunology, Sloan-Kettering Institute for Cancer Research, New York 10021; and the Viral Carcinogenesis Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadao","family":"Aoki","sequence":"additional","affiliation":[{"name":"From The Hospital for Special Surgery and The Philip D. Wilson Research Foundation, Affiliated with The New York Hospital-Cornell University Medical College, and the Department of Pathology, Cornell University Medical College, New York 10021; the Division of Immunology, Sloan-Kettering Institute for Cancer Research, New York 10021; and the Viral Carcinogenesis Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Huebner","sequence":"additional","affiliation":[{"name":"From The Hospital for Special Surgery and The Philip D. Wilson Research Foundation, Affiliated with The New York Hospital-Cornell University Medical College, and the Department of Pathology, Cornell University Medical College, New York 10021; the Division of Immunology, Sloan-Kettering Institute for Cancer Research, New York 10021; and the Viral Carcinogenesis Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1969,5,1]]},"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/129\/5\/1045\/1653188\/1045.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/129\/5\/1045\/1653188\/1045.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T20:30:56Z","timestamp":1690230656000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/129\/5\/1045\/5746\/FURTHER-IMPLICATION-OF-MURINE-LEUKEMIA-LIKE-VIRUS"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1969,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1969,5,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.129.5.1045","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[1969,5,1]]}}}