{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T22:19:37Z","timestamp":1705011577064},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1972,5,1]]},"abstract":"<jats:p>CBA mice were rendered highly tolerant to A\/Jax cells by neonatal intravenous injections of (CBA x A)F1 spleen cells. The high degree of tolerance was ascertained by the absence of circulating antibodies detected in the sera by the usual tests and by the perfect state of A skin grafts during all the experiments. Tolerant sera (sera from tolerant animals) were studied at three periods of tolerance: before skin test grafting, from 2 to 11 wk after grafting, and at time of sacrifice at almost 6 months of age.<\/jats:p>\n               <jats:p>The tolerant sera were shown to have specific facilitation-enhancing properties promoting the take and growth of A\/Jax sarcoma (SaI and \/Sa 15091a grafted on normal CBA mice. These properties were present throughout the duration of the experiments, showing that they were not the result of a beginning interruption of tolerance. The tolerant sera, although lacking the usual serological properties (hemagglutination, hemolysis, cytotoxicity, passive cutaneous anaphylaxis) had, however, specific synergistic hemagglutinating properties (increasing the hemagglutinating titer of a reference immune serum). Antibodies giving direct specific hemagglutination could be extracted from spleens of 20% of highly tolerant mice. The tolerant sera were also found to contain more IgG1 and more IgA than normal sera while they contained normal quantities of the complement-fixing immunoglobulins IgG2 and IgM.<\/jats:p>\n               <jats:p>Fractionation of tolerant sera on DEAE chromatography column confirmed the data concerning immunoglobulin classes and demonstrated direct specific serological activities undetected in unfractionated sera: a weak hemolysis in the most cationic fractions and a weak hemagglutination in the middle fractions. Synergistic hemagglutination, detected in unfractionated serum, was localized in fast anionic fractions containing high IgA concentration, along with facilitation-enhancing activity, thus confirming a link suggested previously between these three properties.<\/jats:p>\n               <jats:p>The relation between immunological tolerance and facilitating antibodies was discussed in the light of the fact that antibodies, possibly of a particular class continuously present at low dose in the sera of highly tolerant animals, are able to transfer (at least partly) this state of tolerance provided a sensitive test system is utilized.<\/jats:p>","DOI":"10.1084\/jem.135.5.1185","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T23:23:31Z","timestamp":1088033011000},"page":"1185-1203","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":23,"title":["IMMUNE STATUS OF MICE TOLERANT OF LIVING CELLS"],"prefix":"10.1084","volume":"135","author":[{"given":"G. A.","family":"Voisin","sequence":"first","affiliation":[{"name":"From the Centre d'Immuno-Pathologie et d'Immunologie Expe\u0301rimentale de l'Association Claude-Bernard et de l'I.N.S.E.R.M., Ho\u0302pital Saint-Antoine, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. G.","family":"Kinsky","sequence":"additional","affiliation":[{"name":"From the Centre d'Immuno-Pathologie et d'Immunologie Expe\u0301rimentale de l'Association Claude-Bernard et de l'I.N.S.E.R.M., Ho\u0302pital Saint-Antoine, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. T.","family":"Duc","sequence":"additional","affiliation":[{"name":"From the Centre d'Immuno-Pathologie et d'Immunologie Expe\u0301rimentale de l'Association Claude-Bernard et de l'I.N.S.E.R.M., Ho\u0302pital Saint-Antoine, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1972,5,1]]},"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/135\/5\/1185\/1654646\/1185.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/135\/5\/1185\/1654646\/1185.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T20:54:33Z","timestamp":1690232073000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/135\/5\/1185\/21800\/IMMUNE-STATUS-OF-MICE-TOLERANT-OF-LIVING-CELLS-II"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1972,5,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.135.5.1185","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[1972,5,1]]}}}