{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T21:05:20Z","timestamp":1780520720729,"version":"3.54.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1972,11,1]]},"abstract":"<jats:p>The ultrastructural features of B-, T-, and surface Ig(sIg)-bearing cells have been studied on cell suspensions from lymphoid organs of mice at different stages of immunization. The cells were identified by exposure to rabbit antibodies against mouse-specific lymphocyte antigens (MSLA) or brain-associated \u03b8 antigen (BA\u03b8) for T cells, mouse-specific bone marrow-derived lymphocyte antigens (MBLA) for B cells, and mouse Ig for sIg-bearing cells. The rabbit antibodies fixed on the cell surfaces were detected by peroxidase-labeled sheep anti-rabbit Ig antibodies or by a \"bridge\" technique using southern bean mosaic virus or bacteriophage T4 as the final markers. In some experiments, short-lived lymphoid cells were labeled in vivo with repeated tritiated thymidine and the ultrastructural detection of their surface antigens was combined with radioautography.<\/jats:p>\n               <jats:p>MBLA+ lymphoid cells showed a whole range of ultrastructural patterns. Most were small and medium-sized lymphocytes with a clear cytoplasm containing mono- and polyribosomes, but they comprised also blasts and large cells with various amounts of endoplasmic reticulum, as well as plasma cells at different stages of maturation. sIg-bearing cells appeared to be identical with MBLA+ cells, except that sIg was less easily detectable on large blasts, and only very rarely observed on plasma cells. MSLA+ and BA\u03b8+ cells fell into three categories. One of them (T1 cells) consisted of small to medium-sized lymphocytes with a clear cytoplasm and few organelles, mostly monoribosomes. A second consisted of large cells (T2 cells) characterized by numerous polyribosomes often in a \"rosette\"-like pattern, occasional dark, membrane-bound granules, and a developing \"filamentous network.\" The third, very characteristic type, (T3 cells) was represented by dark small to medium-sized lymphocytes, usually containing large amounts of closely packed ribosomes and showing a striking accumulation of filamentous network, often condensed in large areas devoid of cell organelles. This filamentous network appeared to correspond to the cytochalasin B-sensitive system of microfilaments found in other cells and considered to be one of the contractile elements of the cell. The T3 lymphocytes showed frequently vesicles suggestive of a strong pinocytic activity, and assumed a variety of shapes, including uropods. Evidence is presented that T1 lymphocytes represent \"virgin\" T cells, T2 \"activated,\" and T3 \"differentiated\" lymphocytes.<\/jats:p>","DOI":"10.1084\/jem.136.5.1008","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T23:23:31Z","timestamp":1088033011000},"page":"1008-1030","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":61,"title":["MOUSE THYMUS-INDEPENDENT AND THYMUS-DERIVED LYMPHOID CELLS"],"prefix":"10.1084","volume":"136","author":[{"given":"A.","family":"Matter","sequence":"first","affiliation":[{"name":"From the Department of Pathology and Institute of Histology, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Lisowska-Bernstein","sequence":"additional","affiliation":[{"name":"From the Department of Pathology and Institute of Histology, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. E.","family":"Ryser","sequence":"additional","affiliation":[{"name":"From the Department of Pathology and Institute of Histology, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-P.","family":"Lamelin","sequence":"additional","affiliation":[{"name":"From the Department of Pathology and Institute of Histology, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Vassalli","sequence":"additional","affiliation":[{"name":"From the Department of Pathology and Institute of Histology, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1972,11,1]]},"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/136\/5\/1008\/1655049\/1008.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/136\/5\/1008\/1655049\/1008.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T21:00:31Z","timestamp":1690232431000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/136\/5\/1008\/6300\/MOUSE-THYMUS-INDEPENDENT-AND-THYMUS-DERIVED"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1972,11,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.136.5.1008","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[1972,11,1]]}}}