{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T18:13:09Z","timestamp":1699639989939},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1973,2,1]]},"abstract":"<jats:p>Both thymus-derived (T) and bone marrow-derived (B) lymphocytes participate in the response to a hapten 4-hydroxy-3-iodo-5-nitrophenylacetic acid (NIP), coupled to a nonimmunogenic isologous carrier, mouse gamma globulin (MGG). Spleen cells from mice immunized with NIP-MGG show increased DNA synthesis in vitro when cultured with NIP-MGG. The participation of and requirement for T cells in the response was demonstrated by treating the spleen cells with anti-\u03b8 serum. This treatment resulted in a 77% inhibition of the antigen response. Furthermore, adoptively transferred normal thymus cells could be specifically \"activated\" by NIP-MGG in vivo and they responded secondarily to the antigen in vitro.<\/jats:p>\n               <jats:p>The active participation of B cells in the secondary response was demonstrated by passing the immune spleen cells through a column coated with polyvalent anti-MGG serum. Column filtration reduced the number of NIP-specific plaque-forming cells and NIP-specific rosette-forming cells (both functions of B cells) and produced a 47% inhibition of the NIP-MGG response. The ability of the cells to respond to phytohemagglutinin (PHA) was not affected by column filtration showing that T cells were not being selectively removed. The participation of B cells in the in vitro NIP-MGG response was also shown by treatment of the spleen cells with antiserum specific for MGG and MGG determinants. B cells were removed by treatment with anti-IgM or polyvalent anti-MGG serum plus complement, resulting in a respective 46 and 49% inhibition of the response to NIP-MGG. (Treatment with anti-IgM serum had no effect on T cells.)<\/jats:p>\n               <jats:p>The contribution of the hapten NIP to stimulation of T cells was investigated using NIP-MGG-activated thymus cells. These activated T cells responded in vitro very well to the NIP-MGG complex but not to the MGG carrier alone demonstrating the requirement of the hapten for T cell stimulation. The response was also partially inhibited (41%) by incubating the activated cells with NIP coupled to a single amino acid (epsilon-aminocaproic acid) before addition of NIP-MGG. These results demonstrated that T cells recognize the hapten NIP when it is coupled to the isologous carrier MGG.<\/jats:p>","DOI":"10.1084\/jem.137.2.411","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T23:23:31Z","timestamp":1088033011000},"page":"411-423","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":27,"title":["IMMUNOLOGIC REACTIONS TO HAPTENS ON AUTOLOGOUS CARRIERS"],"prefix":"10.1084","volume":"137","author":[{"given":"John W.","family":"Moorhead","sequence":"first","affiliation":[{"name":"From the Division of Clinical Immunology, Departments of Medicine and Microbiology, University of Colorado Medical Center, Denver, Colorado 80220"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Curla S.","family":"Walters","sequence":"additional","affiliation":[{"name":"From the Division of Clinical Immunology, Departments of Medicine and Microbiology, University of Colorado Medical Center, Denver, Colorado 80220"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry N.","family":"Claman","sequence":"additional","affiliation":[{"name":"From the Division of Clinical Immunology, Departments of Medicine and Microbiology, University of Colorado Medical Center, Denver, Colorado 80220"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1973,2,1]]},"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/137\/2\/411\/1655320\/411.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/137\/2\/411\/1655320\/411.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T21:04:41Z","timestamp":1690232681000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/137\/2\/411\/6361\/IMMUNOLOGIC-REACTIONS-TO-HAPTENS-ON-AUTOLOGOUS"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1973,2,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.137.2.411","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[1973,2,1]]}}}