{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,7]],"date-time":"2024-09-07T20:39:53Z","timestamp":1725741593411},"reference-count":0,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1973,4,1]]},"abstract":"<jats:p>Antigen-binding T and B lymphocytes were studied by combined autoradiography and immunofluorescence; mouse spleen lymphocytes binding the antigens, [125I]MSH or [125I]TIGAL, were incubated with rhodamine-labeled anti-Ig reagents or with a rhodamine-labeled IgG fraction of anti-\u03b8 serum. B cells were identified as Ig+ or \u03b8-, T cells as Ig- or \u03b8+.<\/jats:p>\n               <jats:p>It was found that: (a) 20% (1\u20132 mo after priming) to 30% (3.5\u20134 mo after priming) of the antigen-binding cells were T cells. (b) The range of antigen molecules bound by B and T cells was similar. (c) Binding of antigen to B and T cells was inhibited by polyvalent anti-Ig, anti-\u00b5, or anti-L reagents. Binding to T cells was more readily inhibited than to B cells. Normal rabbit serum, antimouse lymphocyte serum, or anti-\u03b8 did not inhibit antigen binding. (d) When Ig at the surface of B cells was induced, by noninhibiting concentrations of anti-Ig reagents, to redistribute into polar caps and the cells subsequently exposed to [125I)antigen under noncapping conditions, the [125I]antigen silver grains were distributed in caps superimposed on the Ig fluorescent cap. Of crucial importance, antigen was found in cap in the same proportion of T cells as B cells. Significant capping of antigen receptors was not induced in B or T cells with normal rabbit serum or by anti-Ig reagents absorbed with mouse Ig.<\/jats:p>\n               <jats:p>The main conclusions of this series of experiments using direct visualization of antigen-binding B and T lymphocytes is that T cells have antigen-specific receptors, probably of IgM nature, and that the number of these receptors appears to range in the order of thousands.<\/jats:p>","DOI":"10.1084\/jem.137.4.1060","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T23:23:31Z","timestamp":1088033011000},"page":"1060-1077","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":76,"title":["BLOCKING AND REDISTRIBUTION (\"CAPPING\") OF ANTIGEN RECEPTORS ON T AND B LYMPHOCYTES BY ANTI-IMMUNOGLOBULIN ANTIBODY"],"prefix":"10.1084","volume":"137","author":[{"given":"Georges","family":"Roelants","sequence":"first","affiliation":[{"name":"From the Basel Institute for Immunology, Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana","family":"Forni","sequence":"additional","affiliation":[{"name":"From the Basel Institute for Immunology, Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benvenuto","family":"Pernis","sequence":"additional","affiliation":[{"name":"From the Basel Institute for Immunology, Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1973,4,1]]},"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/137\/4\/1060\/1655296\/1060.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/137\/4\/1060\/1655296\/1060.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T21:04:17Z","timestamp":1690232657000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/137\/4\/1060\/6387\/BLOCKING-AND-REDISTRIBUTION-CAPPING-OF-ANTIGEN"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1973,4,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.137.4.1060","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[1973,4,1]]}}}