{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T03:26:46Z","timestamp":1771644406243,"version":"3.50.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1977,6,1]]},"abstract":"<jats:p>Frequencies of mitogen-reactive B cells have been determined in vitro under culture conditions which allow every growth-inducible B cell to grow and mature into a clone of Ig-secreting PFC.<\/jats:p>\n               <jats:p>The frequencies of LPS-reactive B cells in the spleen of 6- to 8-wk old mice were between 1 in 3 and 1 in 10 splenic B cells from the following inbred strains of mice: C3H\/Tif; BALB\/c; BALB\/c \u03bd\/\u03bd; C57BL\/6J; DBA\/2J; C57BL\/6J x DBA\/(2J)F(1); and CBA and A\/J. Very similar frequencies are found for lipoprotein-reactive B cells in BALB\/c, BALB\/c \u03bd\/\u03bd, C3H\/Tif, and C3H\/HeJ mice. No LPS-reactive cells but normal frequencies of lipoprotein-reactive cells were found in C3H\/HeJ mice, genetically nonreactive to LPS. SJL mice had significantly lower frequencies of LPS- and of lipoprotein-reactive B cells (1 in approximately 30 B cells).<\/jats:p>\n               <jats:p>The number of LPS- and of lipoprotein-reactive B cells in spleen was dependent upon the age of the mouse. Newborn spleen contained approximately 10 percent of the number of reactive cells found at 6- to 8-wk of age. From there the frequencies declined again to drop below 5 percent of the maximal number at ages beyond 11 mo.<\/jats:p>\n               <jats:p>LPS-reactive B cells yielding IgM- and IgG-PFC responses could be found in mesenteric lymph nodes, bone marrow, thymus, thoracic duct, and peripheral blood of 6- to 8-wk old mice. Their frequencies were one in three to five lymph node cells, 1 in 50 to 100 bone marrow cells, one in 10(5) thymus cells, and 1 in 20 to 40 thoracic duct or peripheral blood cells.<\/jats:p>","DOI":"10.1084\/jem.145.6.1511","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T21:00:50Z","timestamp":1088024450000},"page":"1511-1519","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":115,"title":["Frequencies of mitogen-reactive B cells in the mouse. I. Distribution in different lymphoid organs from different inbred strains of mice at different ages"],"prefix":"10.1084","volume":"145","author":[{"given":"J","family":"Andersson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Coutinho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Melchers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1977,6,1]]},"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/145\/6\/1511\/1658519\/1511.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/145\/6\/1511\/1658519\/1511.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T23:09:48Z","timestamp":1690240188000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/145\/6\/1511\/48444\/Frequencies-of-mitogen-reactive-B-cells-in-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1977,6,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.145.6.1511","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[1977,6,1]]}}}