{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T21:02:20Z","timestamp":1700514140015},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1979,12,1]]},"abstract":"<jats:p>The X-linked CBA\/N defect in B cell function precludes an antibody response to phosphorylcholine (PC). Accordingly, (CBA\/N X BALB\/c)F1 male mice are unresponsive to PC and lack circulating immunoglobulin bearing the T15 idiotype characteristic of BALB\/C anti-PC antibody. In contrast, (CBA\/N X BALB\/c)F1 female mice respond to PC and greater than 80% of the anti-PC antibody is T15+. No T-cell abnormalities are known to be associated with the CBA\/N mutation. These experiments compared the ability of helper T cells from either (CBA\/N X BALB\/c)F1 male (T15-) or F1 female (T15+) mice to help F1 female B cells respond to PC and to influence the level of T15 expression. The results indicate that although F1 male T cells collaborated with F1 female B cells just as efficiently as F1 female T cells for the total anti-PC response, the percentage of T15 expression induced by F1 male T cells fell dramatically. The (CBA\/N X BALB\/c)F1 male thus appear to lack a helper T-cell subset required for dominant idiotype production. This helper T cell defect could be repaired by adding F1 female T cells primed to a second carrier to F1 male T cells and restimulating the cell mixture with PC coupled to the antigen used to prime the F1 male cells plus free second carrier. This result implies that conventional helper T cells derived from the F1 male donor can collaborate with a distinct helper T-cell subset from the F1 female donor which recognizes both carrier and idiotype to induce an anti-PC antibody response dominated by the T15 clonotype.<\/jats:p>","DOI":"10.1084\/jem.150.6.1399","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T21:00:50Z","timestamp":1088024450000},"page":"1399-1409","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":66,"title":["Mice whose B cells cannot produce the T15 idiotype also lack an antigen-specific helper T cell required for T15 expression."],"prefix":"10.1084","volume":"150","author":[{"given":"K","family":"Bottomly","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D E","family":"Mosier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1979,12,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/150\/6\/1399\/1660610\/1399.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/150\/6\/1399\/1660610\/1399.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:25Z","timestamp":1690243225000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/150\/6\/1399\/22739\/Mice-whose-B-cells-cannot-produce-the-T15-idiotype"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1979,12,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.150.6.1399","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,12,1]]}}}