{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T11:26:05Z","timestamp":1744370765230},"reference-count":0,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1984,9,1]]},"abstract":"<jats:p>Human T cell clones and a cDNA probe specific for constant regions of the beta subunit of the antigen\/major histocompatibility complex (MHC) receptor, TiC beta 1 and TiC beta 2, were employed to determine whether these genes were differentially used by functional classes of T lymphocytes. DNA from 10 interleukin-2-dependent T cell clones including class I and class II MHC-specific cytotoxic T lymphocytes (n = 6), T4+ inducer T lymphocytes (n = 2), and T8+ suppressor T lymphocytes (n = 2) showed rearrangement of the TiC beta 1 gene on Southern blot analysis with or without deletion of the other TiC beta 1 allele. In contrast, TiC beta 2 always remained in germline configuration. Moreover, the finding that one additional suppressor clone deleted both TiC beta 1 alleles, maintained a germline TiC beta 2 configuration, and yet actively transcribed TiC beta 2 message suggested that TiC beta 2 is not a pseudogene. Rather, it appeared to be used less frequently than the TiC beta 1 gene and in the absence of detectable DNA rearrangements. Together, these results demonstrate that the functional repertoire (or isotype) of a given subclass of T cells is not encoded within the Ti beta genes.<\/jats:p>","DOI":"10.1084\/jem.160.3.947","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T21:00:50Z","timestamp":1088024450000},"page":"947-952","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":33,"title":["Functional isotypes are not encoded by the constant region genes of the beta subunit of the T cell receptor for antigen\/major histocompatibility complex."],"prefix":"10.1084","volume":"160","author":[{"given":"H D","family":"Royer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Bensussan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Acuto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E L","family":"Reinherz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1984,9,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/160\/3\/947\/1665253\/947.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/160\/3\/947\/1665253\/947.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:51:56Z","timestamp":1690246316000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/160\/3\/947\/57246\/Functional-isotypes-are-not-encoded-by-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1984,9,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.160.3.947","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,9,1]]}}}