{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T08:42:21Z","timestamp":1762504941845},"reference-count":0,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1987,10,1]]},"abstract":"<jats:p>Lymphocyte function-associated antigen 3 (LFA-3) is a widely distributed cell surface glycoprotein that binds to the T lymphocyte CD2 surface glycoprotein. This interaction mediates CTL-target cell conjugate formation and adhesion of thymocytes to thymic epithelial cells. CD2 is also the E rosette receptor of T lymphocytes and mediates rosetting with autologous E by binding to LFA-3. We describe deficient expression of LFA-3 on E from paroxysmal nocturnal hemoglobinuria (PNH) patients. PNH is an acquired defect affecting phosphatidylinositol-anchored membrane proteins, of which decay-accelerating factor (DAF) is most important in the clinical symptoms of PNH. LFA-3-negative, weakly positive, and positive populations were found among PNH E. There was a good correlation with DAF deficiency. PNH E exhibited decreased binding of 125I-CD2 and rosetting with a human T lymphoma cell line. PNH E readily incorporated purified LFA-3, restoring LFA-3 expression and the CD2 binding and rosetting activity to normal levels. The expression of DAF was not restored after the incorporation of purified LFA-3 into PNH E, showing that LFA-3 and DAF are different molecules. Phosphatidylinositol-specific phospholipase C (PIPLC) treatment of a B lymphoma cell line released 35% of the cell surface LFA-3 and 62% of DAF. LFA-3 on E was resistant to PIPLC. However, when LFA-3 purified from human E was reconstituted in sheep E or human E and subjected to PIPLC treatment, 40-50% of LFA-3 was released from the cell membrane. The results show that LFA-3 is attached to the cell membrane by a phosphatidylinositol glycolipid moiety, and confirm previous findings (37-41) that LFA-3 is a cell adhesion molecule that mediates adhesion by interacting with CD2 antigen.<\/jats:p>","DOI":"10.1084\/jem.166.4.1011","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1011-1025","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":84,"title":["Deficiency of lymphocyte function-associated antigen 3 (LFA-3) in paroxysmal nocturnal hemoglobinuria. Functional correlates and evidence for a phosphatidylinositol membrane anchor."],"prefix":"10.1084","volume":"166","author":[{"given":"P","family":"Selvaraj","sequence":"first","affiliation":[{"name":"Laboratory of Membrane Immunochemistry, Dana Farber Cancer Institute, Boston, Massachusetts."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M L","family":"Dustin","sequence":"additional","affiliation":[{"name":"Laboratory of Membrane Immunochemistry, Dana Farber Cancer Institute, Boston, Massachusetts."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Silber","sequence":"additional","affiliation":[{"name":"Laboratory of Membrane Immunochemistry, Dana Farber Cancer Institute, Boston, Massachusetts."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M G","family":"Low","sequence":"additional","affiliation":[{"name":"Laboratory of Membrane Immunochemistry, Dana Farber Cancer Institute, Boston, Massachusetts."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T A","family":"Springer","sequence":"additional","affiliation":[{"name":"Laboratory of Membrane Immunochemistry, Dana Farber Cancer Institute, Boston, Massachusetts."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1987,10,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/166\/4\/1011\/1667925\/1011.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/166\/4\/1011\/1667925\/1011.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:12:20Z","timestamp":1690247540000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/166\/4\/1011\/58888\/Deficiency-of-lymphocyte-function-associated"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1987,10,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.166.4.1011","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,10,1]]}}}