{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T05:23:56Z","timestamp":1774675436081,"version":"3.50.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1987,10,1]]},"abstract":"<jats:p>B cell stimulatory factor 2 receptors (BSF-2-R) were studied using radioiodinated recombinant BSF-2 with a specific activity of 6.16 X 10(13) cpm\/g. Kinetic studies showed that binding of 125I-BSF-2 to CESS cells reached maximum level within 150 min at 0 degrees C. There was a single class of receptors with high affinity (Kd 3.4 X 10(-10) M) on CESS, and the number of receptors was 2,700 per cell. Binding of 125I-BSF-2 to CESS was competitively inhibited by unlabeled BSF-2 but not by IL-1, IL-2, IFN-beta, IFN-gamma, and G-CSF, indicating the presence of the receptors specific for BSF-2. EBV-transformed B lymphoblastoid cell lines (CESS, SKW6-CL4, LCL13, and LCL14) expressed BSF-2-R, whereas Burkitt's lines did not. EBV or EBNA2 did not induce the expression of the receptors on Burkitt's cells. The plasma cell lines (ARH-77 and U266) expressed BSF-2-R, fitting the function of BSF-2 as plasma cell growth factor. Several other cell lines, the histiocytic line U937, the promyelocytic line HL60, the astrocytoma line U373 and the glioblastoma line SK-MG-4, in which BSF-2 was inducible with IL-1 or TPA, displayed BSF-2-R with Kd in the range of 1.3-6.4 X 10(-10) M, suggesting the autocrine mechanism in BSF-2 function. The four T cell lines (CEM, HSB, Jurkat, and OM 1) did not express a detectable number of receptors, but normal resting T cells expressed 100-1,000 receptors per cell. BSF-2-R were not present on normal resting B cells but expressed on activated B cells with a Kd of 3.6-5.0 X 10(-10) M, fitting the function of BSF-2, which acts on B cells at the final maturation stage to induce immunoglobulin production.<\/jats:p>","DOI":"10.1084\/jem.166.4.967","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"967-981","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":231,"title":["Receptors for B cell stimulatory factor 2. Quantitation, specificity, distribution, and regulation of their expression."],"prefix":"10.1084","volume":"166","author":[{"given":"T","family":"Taga","sequence":"first","affiliation":[{"name":"Division of Immunology, Osaka University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Kawanishi","sequence":"additional","affiliation":[{"name":"Division of Immunology, Osaka University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R R","family":"Hardy","sequence":"additional","affiliation":[{"name":"Division of Immunology, Osaka University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Hirano","sequence":"additional","affiliation":[{"name":"Division of Immunology, Osaka University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Kishimoto","sequence":"additional","affiliation":[{"name":"Division of Immunology, Osaka University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1987,10,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/166\/4\/967\/1667870\/967.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/166\/4\/967\/1667870\/967.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:11:56Z","timestamp":1690247516000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/166\/4\/967\/58879\/Receptors-for-B-cell-stimulatory-factor-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1987,10,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.166.4.967","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,10,1]]}}}