{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:31:38Z","timestamp":1789997498308,"version":"4.0.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,7,1]]},"abstract":"<jats:p>Experimental allergic encephalomyelitis (EAE) is an autoimmune demyelinating disease of the central nervous system (CNS) that occurs after immunization of animals with myelin basic protein (MBP). The disease is a prototype model for the study of antigen-specific T helper cell-mediated autoimmune disease. In SJL\/J mice, EAE is mediated by T helper cells directed against a 40-amino acid COOH-terminal peptic fragment of mouse small MBP. To identify the minimal T cell epitopes of MBP responsible for EAE, overlapping peptides completely encompassing the epitopes within this region were synthesized. A 28-residue peptide of mouse MBP spanning residues 87-114 (pM87-114) was able to elicit both a strong T cell response and chronic relapsing disease. To better localize the T cell epitopes, shorter peptides within this region were synthesized and two overlapping peptides, pM87-98 and pM91-104, were able to induce EAE. T cell clones and bulk lymph node cell populations reactive with pM87-98 did not respond to pM91-104. However, lymph node cells reactive with pM91-104 also reacted with pM87-98, thus showing that these two peptides represent contiguous, but distinct encephalitogenic epitopes and that both these epitopes may be contained within pM87-98. In addition, pM87-114 and pM87-98 were found to be minor determinants of the total T cell response to rat and rabbit MBP. The restricted response to MBP in SJL\/J mice is similar to that of the PL\/J mice in that each appears to have only a single peptide region in MBP that elicits encephalitogenic T cells. However, within the region studied, there were two if not more T cell epitopes. This differs from the single encephalitogenic PL\/J epitope. These findings of a single encephalitogenic peptide region with multiple T cell epitopes and the fact that encephalitogenic T cell epitopes may be subdominant have implications for the design of treatments directed at the T cell receptor-MHC-peptide epitope complex in autoimmune disease.<\/jats:p>","DOI":"10.1084\/jem.168.1.213","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"213-227","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":117,"title":["Two minor determinants of myelin basic protein induce experimental allergic encephalomyelitis in SJL\/J mice."],"prefix":"10.1084","volume":"168","author":[{"given":"D H","family":"Kono","sequence":"first","affiliation":[{"name":"Division of Biology, California Institute of Technology, Pasadena 91125."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J L","family":"Urban","sequence":"additional","affiliation":[{"name":"Division of Biology, California Institute of Technology, Pasadena 91125."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S J","family":"Horvath","sequence":"additional","affiliation":[{"name":"Division of Biology, California Institute of Technology, Pasadena 91125."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D G","family":"Ando","sequence":"additional","affiliation":[{"name":"Division of Biology, California Institute of Technology, Pasadena 91125."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R A","family":"Saavedra","sequence":"additional","affiliation":[{"name":"Division of Biology, California Institute of Technology, Pasadena 91125."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Hood","sequence":"additional","affiliation":[{"name":"Division of Biology, California Institute of Technology, Pasadena 91125."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1988,7,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/168\/1\/213\/1668992\/213.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/168\/1\/213\/1668992\/213.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:22:53Z","timestamp":1690248173000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/168\/1\/213\/49541\/Two-minor-determinants-of-myelin-basic-protein"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,7,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.168.1.213","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,7,1]]}}}