{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:19:51Z","timestamp":1791328791068,"version":"4.3.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,8,1]]},"abstract":"<jats:p>A small number of B cells are found in the thymus of normal mice. A population of B lymphocytes could be enriched to greater than 90% purity by isolating a low-density fraction on Percoll density gradients and then depleting T cells with a mixture of anti-Thy-1, CD4, and CD8 mAbs and complement. Enrichment was monitored by surface Ig staining and by functional studies (responsiveness to LPS, and to anti-mu plus IL-4). When the phenotype of these B cells was studied by flow cytometry, 60-80% had the phenotype Ly-1+ (CD5), Ia+, B220low (CD45R), and Mac-1+ (CD 11b). In contrast, splenic B cells lacked CD5 and CD11b and expressed higher levels of B220 and Ia antigens. These results indicate that most thymic B cells have the phenotype of the Ly-1 B cell subset, which was identified previously as a trace subpopulation in some peripheral tissues and is thought to play a role in autoantibody formation.<\/jats:p>","DOI":"10.1084\/jem.168.2.811","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"811-816","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":95,"title":["Unusual phenotype of B cells in the thymus of normal mice."],"prefix":"10.1084","volume":"168","author":[{"given":"M","family":"Miyama-Inaba","sequence":"first","affiliation":[{"name":"First Department of Pathology, Kansai Medical University, Moriguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Kuma","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Kansai Medical University, Moriguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Inaba","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Kansai Medical University, Moriguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Ogata","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Kansai Medical University, Moriguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Iwai","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Kansai Medical University, Moriguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Yasumizu","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Kansai Medical University, Moriguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Muramatsu","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Kansai Medical University, Moriguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R M","family":"Steinman","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Kansai Medical University, Moriguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Ikehara","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Kansai Medical University, Moriguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1988,8,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/168\/2\/811\/1668853\/811.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/168\/2\/811\/1668853\/811.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:21:07Z","timestamp":1690248067000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/168\/2\/811\/24074\/Unusual-phenotype-of-B-cells-in-the-thymus-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1988,8,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.168.2.811","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,8,1]]}}}