{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T05:55:43Z","timestamp":1770702943363,"version":"3.49.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,9,1]]},"abstract":"<jats:p>T cell-replacing factor (TRF)\/IL-5 is a glycosylated polypeptide that acts as a key factor for B cell growth and differentiation. Since IL-5 action is probably mediated by specific cell surface receptor(s), we have characterized the binding of IL-5 to cells using biosynthetically [35S]methionine-labeled IL-5 and 125I-IL-5 that had been prepared using Bolton-Hunter reagent. The radiolabeled IL-5 binds specifically to BCL1-B20 (in vitro line) (a murine chronic B cell leukemic cell line previously shown to differentiate into IgM-secreting cells in response to IL-5) within 10 min at 37 degrees C. There are two classes of binding sites with high affinity (Kd = 66 pM) and low affinity (Kd = 12 nM) for IL-5 and an average number of binding sites for high affinity and for low affinity were 400 and 7,500 per cell, respectively. The specificity of binding of radiolabeled IL-5 has been confirmed by demonstrating that only unlabeled IL-5 and anti-IL-5 mAb but not by IL-1, IL-2, IL-3, IFN-gamma, and GM-CSF inhibit radiolabeled IL-5 binding to BCL1-B20 cells. Treatment of surface-bound radiolabeled IL-5 with bivalent crosslinkers identified a membrane polypeptide of Mr 46,500 to which IL-5 is crosslinked. A variety of cell types have been surveyed for the capacity to bind specifically radiolabeled IL-5 with high affinity. BCL1 cells MOPC104E (murine myeloma cell line) expressed IL-5-R, whereas BAL. 17 and L10 A (B cell lymphoma) did not. T cell line, mastocytoma cell line, or macrophage tumor cell line did not display detectable levels of IL-5-R. were hardly detectable on normal resting B cells but were expressed on LPS-activated B cells, fitting the function of IL-5 that acts on activated B cells for their differentiation into Ig-secreting cells. Intriguingly, early B cell lines (J-87 and T-88) that grow in the presence of IL-5 expressed significant but low numbers of high-affinity binding sites for IL-5. The biological effects of IL-5 were mediated by high-affinity binding sites. The identification and characterization of IL-5-R should provide new insight into the apparent diverse biological activities of IL-5.<\/jats:p>","DOI":"10.1084\/jem.168.3.863","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"863-878","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":58,"title":["Receptors for T cell-replacing factor\/interleukin 5. Specificity, quantitation, and its implication."],"prefix":"10.1084","volume":"168","author":[{"given":"S","family":"Mita","sequence":"first","affiliation":[{"name":"Department of Biology, Kumamoto University Medical School, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Harada","sequence":"additional","affiliation":[{"name":"Department of Biology, Kumamoto University Medical School, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Naomi","sequence":"additional","affiliation":[{"name":"Department of Biology, Kumamoto University Medical School, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Hitoshi","sequence":"additional","affiliation":[{"name":"Department of Biology, Kumamoto University Medical School, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Biology, Kumamoto University Medical School, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Akagi","sequence":"additional","affiliation":[{"name":"Department of Biology, Kumamoto University Medical School, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Tominaga","sequence":"additional","affiliation":[{"name":"Department of Biology, Kumamoto University Medical School, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Takatsu","sequence":"additional","affiliation":[{"name":"Department of Biology, Kumamoto University Medical School, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1988,9,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/168\/3\/863\/1668968\/863.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/168\/3\/863\/1668968\/863.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:22:31Z","timestamp":1690248151000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/168\/3\/863\/24129\/Receptors-for-T-cell-replacing-factor-interleukin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1988,9,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.168.3.863","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,9,1]]}}}