{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:19:01Z","timestamp":1791206341565,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,9,1]]},"abstract":"<jats:p>We investigated the effects of B cell stimulatory factor 2\/interleukin 6 (BSF-2\/IL-6) on the development of murine hemopoietic progenitors using serum-containing culture and serum-free culture. In serum-containing culture, BSF-2 mainly supported multipotential blast cell colonies from spleen cells of normal and 5-fluorouracil (5-FU)-treated mice. In serum-free culture, no colony growth was seen in the presence of BSF-2. Addition of BSF-2 to the serum-free culture containing IL-3 resulted in a significant increase in the number of colonies formed from multipotential progenitors in spleen cells and bone marrow cells of 5-FU-treated mice, whereas no effects were seen on the number of single or oligolineage colonies formed by the spleen cells of normal mice. These results suggested that BSF-2 and IL-3 act synergistically on the multipotential progenitors but not on the maturer progenitors. When BSF-2 was added to a culture containing low concentrations of IL-3 (1 U\/ml, 4 U\/ml), which had little effect on colony formation, the number of total colonies formed by the spleen cells and bone marrow cells of 5-FU-treated mice increased significantly. The combination of BSF-2 and 40 U\/ml of IL-3 resulted in a significant enlargement of GMM colonies. Thus, BSF-2 appears to enhance the sensitivity of multipotential hemopoietic progenitors to IL-3.<\/jats:p>","DOI":"10.1084\/jem.168.3.879","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"879-890","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":96,"title":["Synergism of BSF-2\/interleukin 6 and interleukin 3 on development of multipotential hemopoietic progenitors in serum-free culture."],"prefix":"10.1084","volume":"168","author":[{"given":"K","family":"Koike","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Nakahata","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Takagi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Ishiguro","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Tsuji","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Naganuma","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Okano","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Akiyama","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Akabane","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1988,9,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/168\/3\/879\/1669044\/879.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/168\/3\/879\/1669044\/879.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:23:54Z","timestamp":1690248234000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/168\/3\/879\/24115\/Synergism-of-BSF-2-interleukin-6-and-interleukin-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1988,9,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.168.3.879","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,9,1]]}}}